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Rabbit Anti-GABARAP Monoclonal Antibody (EPR4805) (CBMAB-1741-YC)

Provided herein is a rabbit monoclonal antibody against Human GABARAP. The antibody, clone EPR4805, can be used for immunoassay techniques, such as FC, ICC, IF, IHC-P and WB.
See all GABARAP antibodies

Summary

Host Animal
Rabbit
Specificity
Human, Mouse, Rat, Silk worm
Clone
EPR4805
Antibody Isotype
IgG
Application
FC, ICC, IF, IHC-P, WB

Basic Information

Immunogen
Corresponding to Human GABARAP, GABARAPL1 and GABARAPL2 aa 1-100.
Specificity
Human, Mouse, Rat, Silk worm
Antibody Isotype
IgG
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
GABA Type A Receptor-Associated Protein
Introduction
Gamma-aminobutyric acid A receptors (GABARAP) are ligand-gated chloride channels that mediate inhibitory neurotransmission. GABARAP is highly positively charged in its N-terminus and shares sequence similarity with light chain-3 of microtubule-associated proteins 1A and 1B. GABARAP clusters neurotransmitter receptors by mediating interaction with the cytoskeleton.
Entrez Gene ID
Human11337
Mouse56486
Rat58974
UniProt ID
HumanO95166
MouseQ9DCD6
RatP60517
Alternative Names
MM46; ATG8A; GABARAP-a; ATG8; GEC1; APG8L; ATG8B; ATG8L; APG8-LIKE; GEF2; ATG8C; GEF-2; GATE16; GATE-16
Function
Ubiquitin-like modifier that plays a role in intracellular transport of GABA(A) receptors and its interaction with the cytoskeleton (PubMed:9892355).

Involved in autophagy: while LC3s are involved in elongation of the phagophore membrane, the GABARAP/GATE-16 subfamily is essential for a later stage in autophagosome maturation (PubMed:15169837, PubMed:20562859, PubMed:22948227).

Through its interaction with the reticulophagy receptor TEX264, participates in the remodeling of subdomains of the endoplasmic reticulum into autophagosomes upon nutrient stress, which then fuse with lysosomes for endoplasmic reticulum turnover (PubMed:31006538).

Also required for the local activition of the CUL3(KBTBD6/7) E3 ubiquitin ligase complex, regulating ubiquitination and degradation of TIAM1, a guanyl-nucleotide exchange factor (GEF) that activates RAC1 and downstream signal transduction (PubMed:25684205).

Thereby, regulates different biological processes including the organization of the cytoskeleton, cell migration and proliferation (PubMed:25684205).

Involved in apoptosis (PubMed:15977068).
Biological Process
Autophagosome assembly Source: GO_Central
Autophagy of mitochondrion Source: GO_Central
Cellular response to nitrogen starvation Source: GO_Central
Chemical synaptic transmission Source: ProtInc
Extrinsic apoptotic signaling pathway via death domain receptors Source: UniProtKB
Macroautophagy Source: GO_Central
Microtubule cytoskeleton organization Source: Ensembl
Positive regulation of proteasomal ubiquitin-dependent protein catabolic process Source: UniProtKB
Positive regulation of protein K48-linked ubiquitination Source: UniProtKB
Protein targeting Source: ProtInc
Regulation of Rac protein signal transduction Source: UniProtKB
Cellular Location
Golgi apparatus membrane; Cytoskeleton; Endomembrane system; Autophagosome; Cytoplasmic vesicle. Largely associated with intracellular membrane structures including the Golgi apparatus and postsynaptic cisternae. Colocalizes with microtubules (By similarity). Localizes also to discrete punctae along the ciliary axoneme (By similarity).
PTM
The precursor molecule is cleaved by ATG4B to form the cytosolic form, GABARAP-I (PubMed:15169837). This is activated by APG7L/ATG7, transferred to ATG3 and conjugated to phospholipid to form the membrane-bound form, GABARAP-II (PubMed:15169837).
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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