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Rabbit Anti-GNA13 Recombinant Antibody (CBLG1-2838) (CBMAB-G4059-LY)

This product is antibody recognizes GNA13. The antibody CBLG1-2838 immunoassay techniques such as: WB.
See all GNA13 antibodies

Summary

Host Animal
Rabbit
Specificity
Human, Mouse, Rat
Clone
CBLG1-2838
Antibody Isotype
IgG
Application
WB

Basic Information

Immunogen
A synthetic peptide corresponding to residues in human G protein alpha 13 was used as an immunogen
Specificity
Human, Mouse, Rat
Antibody Isotype
IgG
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
50% Glycerol, 0.05% BSA
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
guanine nucleotide binding protein (G protein), alpha 13
Entrez Gene ID
Human10672
Mouse14674
Rat303634
UniProt ID
HumanQ14344
MouseP27601
RatQ6Q7Y5
Alternative Names
G13
Function
Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems (PubMed:15240885, PubMed:16787920, PubMed:16705036, PubMed:27084452).

Activates effector molecule RhoA by binding and activating RhoGEFs (ARHGEF1/p115RhoGEF, ARHGEF11/PDZ-RhoGEF and ARHGEF12/LARG) (PubMed:15240885, PubMed:12515866).

GNA13-dependent Rho signaling subsequently regulates transcription factor AP-1 (activating protein-1) (By similarity).

Promotes tumor cell invasion and metastasis by activating RhoA/ROCK signaling pathway (PubMed:16787920, PubMed:16705036, PubMed:27084452).

Inhibits CDH1-mediated cell adhesion in process independent from Rho activation (PubMed:11976333).
Biological Process
Activation of phospholipase D activity Source: GO_Central
Adenylate cyclase-activating G protein-coupled receptor signaling pathway Source: GO_Central
Adenylate cyclase-modulating G protein-coupled receptor signaling pathway Source: UniProtKB
Negative regulation of vascular associated smooth muscle cell migration Source: Ensembl
Negative regulation of vascular associated smooth muscle cell proliferation Source: Ensembl
Positive regulation of cytosolic calcium ion concentration Source: Ensembl
Regulation of small GTPase mediated signal transduction Source: Reactome
Rho protein signal transduction Source: GO_Central
Signal transduction Source: ProtInc
Cellular Location
Cell membrane; Cytoplasm; Nucleus; Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV (PubMed:17081065). Detected in the cytoplasm of Leydig cells and in the seminiferous epithelium, including differentiating cells from the spermatogonia to mature spermatozoa stages (PubMed:18703424). In round spermatids, also present in the nuclei (PubMed:18703424).
PTM
Palmitoylation is critical for proper membrane localization and signaling.
Phosphorylation on Thr-203 by PKA destabilizes the heterotrimer of alpha, beta and gamma, and inhibits Rho activation.

Rasheed, S. A. K., Subramanyan, L. V., Lim, W. K., Udayappan, U. K., Wang, M., & Casey, P. J. (2022). The emerging roles of Gα12/13 proteins on the hallmarks of cancer in solid tumors. Oncogene, 41(2), 147-158.

Xia, Z., Zhang, X., Liu, P., Zhang, R., Huang, Z., Li, D., ... & Ren, R. (2021). GNA13 regulates BCL2 expression and the sensitivity of GCB-DLBCL cells to BCL2 inhibitors in a palmitoylation-dependent manner. Cell death & disease, 12(1), 54.

Liu, Y., Du, Z., Xu, Z., Jin, T., Xu, K., Huang, M., ... & Xu, H. (2021). Overexpressed GNA13 induces temozolomide sensitization via down-regulating MGMT and p-RELA in glioma. American Journal of Translational Research, 13(10), 11413.

Goodwin, A. T., Hinz, B., & Jenkins, G. (2021). S77 The G proteins Gαq/11 and Gα12/13 drive unique myofibroblast functions to promote pulmonary fibrosis.

Lim, W. K., Chai, X., Ghosh, S., Ray, D., Wang, M., Rasheed, S. A. K., & Casey, P. J. (2019). Gα-13 induces CXC motif chemokine ligand 5 expression in prostate cancer cells by transactivating NF-κB. Journal of Biological Chemistry, 294(48), 18192-18206.

Shimono, J., Miyoshi, H., Yoshida, N., Kato, T., Sato, K., Sugio, T., ... & Ohshima, K. (2018). Analysis of GNA13 protein in follicular lymphoma and its association with poor prognosis. The American Journal of Surgical Pathology, 42(11), 1466.

Zhang, Z., Tan, X., Luo, J., Cui, B., Lei, S., Si, Z., ... & Yao, H. (2018). GNA 13 promotes tumor growth and angiogenesis by upregulating CXC chemokines via the NF‐κB signaling pathway in colorectal cancer cells. Cancer medicine, 7(11), 5611-5620.

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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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