Rabbit Anti-GNAT2 Recombinant Antibody (EG1339) (CBMAB-EN1617-LY)

The product is antibody recognizes GNAT2. The antibody EG1339 immunoassay techniques such as: WB: 1:500~1:1000 ELISA: 1:10000.
See all GNAT2 antibodies

Datasheet

Summary

Host Animal

Rabbit

Specificity

Human, Mouse

Clone

EG1339

Antibody Isotype

IgG

Application

WB: 1:500~1:1000 ELISA: 1:10000

Basic Information

Immunogen

The antibody was produced against synthesized peptide derived from internal of human GNAT2.

Specificity

Human, Mouse

Antibody Isotype

IgG

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name

G protein subunit alpha transducin 2

Introduction

Transducin is a 3-subunit guanine nucleotide-binding protein (G protein) which stimulates the coupling of rhodopsin and cGMP-phoshodiesterase during visual impulses. The transducin alpha subunits in rods and cones are encoded by separate genes. This gene encodes the alpha subunit in cones.

Entrez Gene ID

Human	2780
Mouse	14686

UniProt ID

Human	P19087
Mouse	P50149

Alternative Names

ACHM4; GNATC

Function

Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems. Transducin is an amplifier and one of the transducers of a visual impulse that performs the coupling between rhodopsin and cGMP-phosphodiesterase.

Biological Process

Adenylate cyclase-modulating G protein-coupled receptor signaling pathway Source: GO_Central
Detection of chemical stimulus involved in sensory perception of bitter taste Source: GO_Central
Detection of light stimulus involved in visual perception Source: UniProtKB
G protein-coupled receptor signaling pathway Source: UniProtKB
Phototransduction Source: UniProtKB
Visual perception Source: UniProtKB

Cellular Location

Photoreceptor outer segmen; Photoreceptor inner segment. Localizes mainly in the outer segment in the dark-adapted state, whereas is translocated to the inner part of the photoreceptors in the light-adapted state. During dark-adapted conditions, in the presence of UNC119 mislocalizes from the outer segment to the inner part of rod photoreceptors which leads to decreased photoreceptor damage caused by light.

Involvement in disease

Achromatopsia 4 (ACHM4):
An ocular stationary disorder due to the absence of functioning cone photoreceptors in the retina. It is characterized by total colorblindness, low visual acuity, photophobia and nystagmus.

References

Rantala, M., Ivanauskaite, A., Laihonen, L., Kanna, S. D., Ughy, B., & Mulo, P. (2022). Chloroplast Acetyltransferase GNAT2 is Involved in the Organization and Dynamics of Thylakoid Structure. Plant and Cell Physiology, 63(9), 1205-1214.

Flood, M. D., Veloz, H. L., Hattar, S., & Carvalho-de-Souza, J. L. (2022). Robust visual cortex evoked potentials (VEP) in Gnat1 and Gnat2 knockout mice. Frontiers in Cellular Neuroscience, 16, 654.

Georgiou, M., Singh, N., Kane, T., Robson, A. G., Kalitzeos, A., Hirji, N., ... & Michaelides, M. (2020). Photoreceptor structure in GNAT2-associated achromatopsia. Investigative Ophthalmology & Visual Science, 61(3), 40-40.

Chen, C. K. J., Chen, Y. J., & Shay, A. (2019). Surprises from modulating GNAT1 and GNAT2 expression by CRISPR/Cas9-mediated gene targeting. Investigative Ophthalmology & Visual Science, 60(9), 1009-1009.

Felden, J., Baumann, B., Ali, M., Audo, I., Ayuso, C., Bocquet, B., ... & Kohl, S. (2019). Mutation spectrum and clinical investigation of achromatopsia patients with mutations in the GNAT2 gene. Human Mutation, 40(8), 1145-1155.

Zahid, S., Branham, K., Schlegel, D., Pennesi, M. E., Michaelides, M., Heckenlively, J., ... & Jayasundera, T. (2018). GNAT2. Retinal dystrophy gene atlas, 103-105.

Ronning, K. E., Allina, G. P., Miller, E. B., Zawadzki, R. J., Pugh Jr, E. N., Herrmann, R., & Burns, M. E. (2018). Loss of cone function without degeneration in a novel Gnat2 knock-out mouse. Experimental eye research, 171, 111-118.

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Protocols

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Associated Products

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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