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Rabbit Anti-GPR18 Recombinant Antibody (EG1396) (CBMAB-EN1675-LY)

The product is antibody recognizes GPR18. The antibody EG1396 immunoassay techniques such as: WB: 1:500~1:1000 IF: 1:100~1:500 ELISA: 1:1000.
See all GPR18 antibodies

Summary

Host Animal
Rabbit
Specificity
Human, Mouse, Rat
Clone
EG1396
Antibody Isotype
IgG
Application
WB: 1:500~1:1000 IF: 1:100~1:500 ELISA: 1:1000

Basic Information

Immunogen
The antibody was produced against synthesized peptide derived from internal of human GPR18.
Specificity
Human, Mouse, Rat
Antibody Isotype
IgG
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
G protein-coupled receptor 18
Entrez Gene ID
Human2841
Mouse110168
Rat679957
UniProt ID
HumanQ14330
MouseQ8K1Z6
RatA1A5S3
Function
Receptor for endocannabinoid N-arachidonyl glycine (NAGly) (PubMed:16844083, PubMed:24762058, PubMed:27572937).

However, conflicting results about the role of NAGly as an agonist are reported (PubMed:27018161).

Can also be activated by plant-derived and synthetic cannabinoid agonists (PubMed:24762058).

The activity of this receptor is mediated by G proteins which inhibit adenylyl cyclase (PubMed:16844083).

May contribute to regulation of the immune system. Is required for normal homeostasis of CD8+ subsets of intraepithelial lymphocytes (IELs) (CD8alphaalpha and CD8alphabeta IELs)in small intstine by supporting preferential migration of CD8alphaalpha T-cells to intraepithelial compartment over lamina propria compartment, and by mediating their reconstitution into small intestine after bone marrow transplant (By similarity).

Plays a role in hypotensive responses, mediating reduction in intraocular and blood pressure (By similarity).

Mediates NAGly-induced process of reorganization of actin filaments and induction of acrosomal exocytosis (PubMed:27572937).
Biological Process
CD8-positive, alpha-beta intraepithelial T cell differentiation Source: Ensembl
CD8-positive, gamma-delta intraepithelial T cell differentiation Source: Ensembl
G protein-coupled receptor signaling pathway Source: UniProtKB
Negative regulation of leukocyte chemotaxis Source: Ensembl
Negative regulation of tumor necrosis factor production Source: Ensembl
Positive regulation of cytosolic calcium ion concentration involved in phospholipase C-activating G protein-coupled signaling Pathway Source: GO_Central
Positive regulation of Rho protein signal transduction Source: GO_Central
Cellular Location
Cell membrane; Cytoplasmic vesicle membrane
Topology
Extracellular: 1-26
Helical: 27-47
Cytoplasmic: 48-56
Helical: 57-77
Extracellular: 78-95
Helical: 96-116
Cytoplasmic: 117-138
Helical: 139-159
Extracellular: 160-191
Helical: 192-212
Cytoplasmic: 213-232
Helical: 233-253
Extracellular: 254-268
Helical: 269-289
Cytoplasmic: 290-331

Bardin, M., Pawelzik, S. C., Lagrange, J., Mahdi, A., Arnardottir, H., Regnault, V., ... & Bäck, M. (2022). The resolvin D2–GPR18 axis is expressed in human coronary atherosclerosis and transduces atheroprotection in apolipoprotein E deficient mice. Biochemical Pharmacology, 201, 115075.

Merlin, J., Park, J., Vandekolk, T. H., Fabb, S. A., Allinne, J., Summers, R. J., ... & Riddy, D. M. (2022). Multipathway In vitro pharmacological characterization of specialized proresolving G protein-coupled receptors. Molecular Pharmacology, 101(4), 246-256.

Zhang, T., Zuo, G., & Zhang, H. (2022). GPR18 agonist resolvin D2 reduces early brain injury in a rat model of subarachnoid hemorrhage by multiple protective mechanisms. Cellular and Molecular Neurobiology, 42(7), 2379-2392.

Mastromarino, M., Lacivita, E., Colabufo, N. A., & Leopoldo, M. (2020). G-protein coupled receptors involved in the resolution of inflammation: Ligands and therapeutic perspectives. Mini Reviews in Medicinal Chemistry, 20(20), 2090-2103.

Schoeder, C. T., Mahardhika, A. B., Drabczyńska, A., Kieć-Kononowicz, K., & Müller, C. E. (2020). Discovery of tricyclic xanthines as agonists of the cannabinoid-activated orphan G-protein-coupled receptor GPR18. ACS Medicinal Chemistry Letters, 11(10), 2024-2031.

Neumann, A., Engel, V., Mahardhika, A. B., Schoeder, C. T., Namasivayam, V., Kieć-Kononowicz, K., & Müller, C. E. (2020). Computational investigations on the binding mode of ligands for the cannabinoid-activated G protein-coupled receptor GPR18. Biomolecules, 10(5), 686.

Reyes-Resina, I., Navarro, G., Aguinaga, D., Canela, E. I., Schoeder, C. T., Załuski, M., ... & Franco, R. (2018). Molecular and functional interaction between GPR18 and cannabinoid CB2 G-protein-coupled receptors. Relevance in neurodegenerative diseases. Biochemical pharmacology, 157, 169-179.

Sumida, H., & Cyster, J. G. (2018). G-protein coupled receptor 18 contributes to establishment of the CD8 effector T cell compartment. Frontiers in immunology, 9, 660.

Schoeder, C. T., Kaleta, M., Mahardhika, A. B., Olejarz-Maciej, A., Łażewska, D., Kieć-Kononowicz, K., & Müller, C. E. (2018). Structure-activity relationships of imidazothiazinones and analogs as antagonists of the cannabinoid-activated orphan G protein-coupled receptor GPR18. European Journal of Medicinal Chemistry, 155, 381-397.

Zhang, L. Y., Liu, Z. H., Zhu, Q., Wen, S., Yang, C. X., Fu, Z. J., & Sun, T. (2018). Resolvin D2 relieving radicular pain is associated with regulation of inflammatory mediators, Akt/GSK-3β signal pathway and GPR18. Neurochemical Research, 43, 2384-2392.

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For research use only. Not intended for any clinical use.

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