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Rabbit Anti-GSPT1 Recombinant Antibody (EG1460) (CBMAB-EN1744-LY)

The product is antibody recognizes GSPT1. The antibody EG1460 immunoassay techniques such as: IHC: 1:50~1:100 ELISA: 1:40000.
See all GSPT1 antibodies

Summary

Host Animal
Rabbit
Specificity
Human, Mouse, Rat
Clone
EG1460
Antibody Isotype
IgG
Application
IHC: 1:50~1:100 ELISA: 1:40000

Basic Information

Immunogen
The antibody was produced against synthesized peptide derived from internal of human GSPT1.
Specificity
Human, Mouse, Rat
Antibody Isotype
IgG
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
G1 to S phase transition 1
Entrez Gene ID
Human2935
Mouse14852
Rat24420
UniProt ID
HumanP15170
MouseQ8R050
RatQ6AYD5
Alternative Names
GST1; ETF3A; eRF3a; 551G9.2
Function
Involved in translation termination in response to the termination codons UAA, UAG and UGA (By similarity).

Stimulates the activity of ETF1 (By similarity).

Involved in regulation of mammalian cell growth (PubMed:2511002).

Component of the transient SURF complex which recruits UPF1 to stalled ribosomes in the context of nonsense-mediated decay (NMD) of mRNAs containing premature stop codons (PubMed:24486019).

Required for SHFL-mediated translation termination which inhibits programmed ribosomal frameshifting (-1PRF) of mRNA from viruses and cellular genes (PubMed:30682371).
Biological Process
Cytoplasmic translational termination Source: GO_Central
G1/S transition of mitotic cell cycle Source: UniProtKB
Nuclear-transcribed mRNA catabolic process, nonsense-mediated decay Source: UniProtKB
Protein methylation Source: MGI
Regulation of translational termination Source: UniProtKB
Translation Source: GO_Central
Translational termination Source: ComplexPortal
Cellular Location
Cytosol; Translation release factor complex

Sellar, R. S., Sperling, A. S., Słabicki, M., Gasser, J. A., McConkey, M. E., Donovan, K. A., ... & Ebert, B. L. (2022). Degradation of GSPT1 causes TP53-independent cell death in leukemia while sparing normal hematopoietic stem cells. The Journal of Clinical Investigation, 132(16).

Hansen, J. D., Correa, M., Alexander, M., Nagy, M., Huang, D., Sapienza, J., ... & Lopez-Girona, A. (2021). CC-90009: a cereblon E3 ligase modulating drug that promotes selective degradation of GSPT1 for the treatment of acute myeloid leukemia. Journal of Medicinal Chemistry, 64(4), 1835-1843.

Nishiguchi, G., Keramatnia, F., Min, J., Chang, Y., Jonchere, B., Das, S., ... & Rankovic, Z. (2021). Identification of potent, selective, and orally bioavailable small-molecule GSPT1/2 degraders from a focused library of cereblon modulators. Journal of Medicinal Chemistry, 64(11), 7296-7311.

Li, Z., Xie, X., Fan, X., & Li, X. (2020). Long non-coding RNA MINCR regulates miR-876-5p/GSPT1 axis to aggravate glioma progression. Neurochemical Research, 45(7), 1690-1699.

Powell, C. E., Du, G., Che, J., He, Z., Donovan, K. A., Yue, H., ... & Gray, N. S. (2020). Selective degradation of GSPT1 by cereblon modulators identified via a focused combinatorial library. ACS chemical biology, 15(10), 2722-2730.

Uy, G. L., Minden, M. D., Montesinos, P., DeAngelo, D. J., Altman, J. K., Koprivnikar, J., ... & Zeidan, A. M. (2019). Clinical activity of CC-90009, a cereblon E3 ligase modulator and first-in-class GSPT1 degrader, as a single agent in patients with relapsed or refractory acute myeloid leukemia (R/R AML): first results from a Phase I dose-finding study. Blood, 134, 232.

Ishoey, M., Chorn, S., Singh, N., Jaeger, M. G., Brand, M., Paulk, J., ... & Winter, G. E. (2018). Translation termination factor GSPT1 is a phenotypically relevant off-target of heterobifunctional phthalimide degraders. ACS Chemical Biology, 13(3), 553-560.

Tian, Q. G., Tian, R. C., Liu, Y., Niu, A. Y., Zhang, J., & Gao, W. F. (2018). The role of miR-144/GSPT1 axis in gastric cancer. Eur Rev Med Pharmacol Sci, 22(13), 4138-4145.

Hansen, J. D., Condroski, K., Correa, M., Muller, G., Man, H. W., Ruchelman, A., ... & Lu, C. C. (2018). Protein degradation via CRL4CRBN ubiquitin ligase: discovery and structure–activity relationships of novel glutarimide analogs that promote degradation of aiolos and/or GSPT1. Journal of Medicinal Chemistry, 61(2), 492-503.

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For research use only. Not intended for any clinical use.

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We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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