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Mouse Anti-PCLAF Recombinant Antibody (3C11-1F11) (CBMAB-A4675-LY)


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Summary

Host Animal
Mouse
Specificity
Human
Clone
3C11-1F11
Antibody Isotype
IgG1, κ
Application
WB, ELISA

Basic Information

Immunogen
KIAA0101 (AAH05832, 1 a.a. ~ 111 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Specificity
Human
Antibody Isotype
IgG1, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Purity
> 95% Purity determined by SDS-PAGE.
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
PCNA Clamp Associated Factor
Entrez Gene ID
9768
UniProt ID
Q15004
Alternative Names
L5; NS5ATP9; OEATC-1; OEATC1; PAF; p15(PAF)
Function
PCNA-binding protein that acts as a regulator of DNA repair during DNA replication. Following DNA damage, the interaction with PCNA is disrupted, facilitating the interaction between monoubiquitinated PCNA and the translesion DNA synthesis DNA polymerase eta (POLH) at stalled replisomes, facilitating the bypass of replication-fork-blocking lesions. Also acts as a regulator of centrosome number.
Biological Process
Cellular response to DNA damage stimulusManual Assertion Based On ExperimentIDA:UniProtKB
Centrosome cycleManual Assertion Based On ExperimentIMP:UniProtKB
DNA replicationManual Assertion Based On ExperimentIDA:UniProtKB
Regulation of cell cycleManual Assertion Based On ExperimentIMP:UniProtKB
Response to UVManual Assertion Based On ExperimentIDA:UniProtKB
Translesion synthesisManual Assertion Based On ExperimentIMP:UniProtKB
Cellular Location
Nucleus
Cytoplasm, perinuclear region
Following DNA damage, localizes to DNA damage sites (PubMed:21628590).
Colocalizes with centrosomes in perinuclear region (PubMed:21673012).
PTM
Monoubiquitinated at Lys-15 and Lys-24 during normal S phase, promoting its association with PCNA. Also diubiquitinated at these 2 sites. Following DNA damage, monoubiquitin chains at Lys-15 and Lys-24 are probably extended, leading to disrupt the interaction with PCNA. Polyubiquitinated by the APC/C complex at the mitotic exit, leading to its degradation by the proteasome.
More Infomation

Li, Y., Zeng, Z. W., Chen, D., Gu, Z. C., Yan, W. L., Yue, L. Y., ... & He, B. (2023). Facilitated Drug Repurposing with Artemisinin-Derived PROTACs: Unveiling PCLAF as a Therapeutic Target. Journal of Medicinal Chemistry, 66(16), 11335-11350.

Liu, L., Lv, Z., Wang, M., Zhang, D., Liu, D., & Zhu, F. (2023). HBV enhances sorafenib resistance in hepatocellular carcinoma by reducing ferroptosis via SRSF2-mediated abnormal PCLAF splicing. International Journal of Molecular Sciences, 24(4), 3263.

Liu, X., Cheng, C., Cai, Y., Gu, Y., Wu, Y., Chen, K., & Wu, Z. (2022). Pan‑cancer analyses reveal the regulation and clinical outcome association of PCLAF in human tumors. International Journal of Oncology, 60(6), 1-13.

Liu, X., Cai, Y., Cheng, C., Gu, Y., Hu, X., Chen, K., ... & Wu, Z. (2022). PCLAF promotes neuroblastoma G1/S cell cycle progression via the E2F1/PTTG1 axis. Cell death & disease, 13(2), 178.

Kim, B., Huang, Y., Ko, K. P., Zhang, S., Zou, G., Zhang, J., ... & Park, J. I. (2022). PCLAF-DREAM Drives Alveolar Cell Plasticity for Lung Regeneration. bioRxiv, 2022-10.

Magallanes-Garza, G. I., Santuario-Facio, S. K., Lira-Albarrán, S., Varela-Varela, A. F., Cardona-Huerta, S., Ruiz-Flores, P., ... & Ortiz-Lopez, R. (2022). NUSAP1 and PCLAF (KIA0101) downregulation by neoadjuvant therapy is associated with better therapeutic outcomes and survival in breast cancer. Journal of Oncology, 2022(1), 6001947.

Pan, L., Sha, J., Lin, W., Wang, Y., Bian, T., & Guo, J. (2021). Curcumin inhibits prostate cancer progression by regulating the miR‑30a‑5p/PCLAF axis. Experimental and Therapeutic Medicine, 22(3), 1-8.

Tantiwetrueangdet, A., Panvichian, R., Sornmayura, P., Leelaudomlipi, S., & Macoska, J. A. (2021). PCNA-associated factor (KIAA0101/PCLAF) overexpression and gene copy number alterations in hepatocellular carcinoma tissues. BMC cancer, 21, 1-12.

Ma, F., Zhi, C., Wang, M., Li, T., Khan, S. A., Ma, Z., ... & Ji, T. (2020). Dysregulated NF-κB signal promotes the hub gene PCLAF expression to facilitate nasopharyngeal carcinoma proliferation and metastasis. Biomedicine & Pharmacotherapy, 125, 109905.

Zhao, H., Zhang, W., Lai, X., Zhu, H., Zhang, S., Wu, W., ... & Tan, J. (2019). MiR-30a-5p frequently downregulated in prostate cancer inhibits cell proliferation via targeting PCLAF. Artificial cells, nanomedicine, and biotechnology, 47(1), 278-289.

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For research use only. Not intended for any clinical use.

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