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Mouse Anti-PCLAF Recombinant Antibody (K11-P1G7/B8) (CBMAB-P1057-YC)

Provided herein is a Mouse monoclonal antibody against Human PCNA clamp associated factor. The antibody can be used for immunoassay techniques, such as WB, ELISA, IHC.
See all PCLAF antibodies

Summary

Host Animal
Mouse
Specificity
Human
Clone
K11-P1G7/B8
Antibody Isotype
IgG2b, κ
Application
WB, ELISA, IHC

Basic Information

Immunogen
Peptide Sequence-RKACPLQPDHT-peptide
Specificity
Human
Antibody Isotype
IgG2b, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at-20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
PCNA Clamp Associated Factor
Introduction
PCLAF has biased expression in bone marrow (RPKM 26.4), lymph node (RPKM 13.8) and 11 other tissues.
Entrez Gene ID
9768
UniProt ID
Q15004
Alternative Names
KIAA0101; L5; NS5ATP9; OEATC; OEATC-1; OEATC1; PAF; PAF15; p15(PAF); p15/PAF; p15PAF
Function
PCNA-binding protein that acts as a regulator of DNA repair during DNA replication. Following DNA damage, the interaction with PCNA is disrupted, facilitating the interaction between monoubiquitinated PCNA and the translesion DNA synthesis DNA polymerase eta (POLH) at stalled replisomes, facilitating the bypass of replication-fork-blocking lesions. Also acts as a regulator of centrosome number.
Biological Process
Cellular response to DNA damage stimulusManual Assertion Based On ExperimentIDA:UniProtKB
Centrosome cycleManual Assertion Based On ExperimentIMP:UniProtKB
DNA replicationManual Assertion Based On ExperimentIDA:UniProtKB
Regulation of cell cycleManual Assertion Based On ExperimentIMP:UniProtKB
Response to UVManual Assertion Based On ExperimentIDA:UniProtKB
Translesion synthesisManual Assertion Based On ExperimentIMP:UniProtKB
Cellular Location
Nucleus
Cytoplasm, perinuclear region
Following DNA damage, localizes to DNA damage sites (PubMed:21628590).
Colocalizes with centrosomes in perinuclear region (PubMed:21673012).
PTM
Monoubiquitinated at Lys-15 and Lys-24 during normal S phase, promoting its association with PCNA. Also diubiquitinated at these 2 sites. Following DNA damage, monoubiquitin chains at Lys-15 and Lys-24 are probably extended, leading to disrupt the interaction with PCNA. Polyubiquitinated by the APC/C complex at the mitotic exit, leading to its degradation by the proteasome.
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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