PCLAF

PCNA-binding protein that acts as a regulator of DNA repair during DNA replication. Following DNA damage, the interaction with PCNA is disrupted, facilitating the interaction between monoubiquitinated PCNA and the translesion DNA synthesis DNA polymerase eta (POLH) at stalled replisomes, facilitating the bypass of replication-fork-blocking lesions. Also acts as a regulator of centrosome number.

PCNA Clamp Associated Factor

PCNA-binding protein that acts as a regulator of DNA repair during DNA replication. Following DNA damage, the interaction with PCNA is disrupted, facilitating the interaction between monoubiquitinated PCNA and the translesion DNA synthesis DNA polymerase eta (POLH) at stalled replisomes, facilitating the bypass of replication-fork-blocking lesions. Also acts as a regulator of centrosome number.

Cellular response to DNA damage stimulusManual Assertion Based On ExperimentIDA:UniProtKB
Centrosome cycleManual Assertion Based On ExperimentIMP:UniProtKB
DNA replicationManual Assertion Based On ExperimentIDA:UniProtKB
Regulation of cell cycleManual Assertion Based On ExperimentIMP:UniProtKB
Response to UVManual Assertion Based On ExperimentIDA:UniProtKB
Translesion synthesisManual Assertion Based On ExperimentIMP:UniProtKB

Nucleus
Cytoplasm, perinuclear region
Following DNA damage, localizes to DNA damage sites (PubMed:21628590).
Colocalizes with centrosomes in perinuclear region (PubMed:21673012).

Monoubiquitinated at Lys-15 and Lys-24 during normal S phase, promoting its association with PCNA. Also diubiquitinated at these 2 sites. Following DNA damage, monoubiquitin chains at Lys-15 and Lys-24 are probably extended, leading to disrupt the interaction with PCNA. Polyubiquitinated by the APC/C complex at the mitotic exit, leading to its degradation by the proteasome.