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Mouse Anti-BPGM Recombinant Antibody (CBYY-1806) (CBMAB-2155-YY)


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Published Data
CBMAB-2155-YY-1
CBMAB-2155-YY in WB
Erythrocyte membrane and cytosolic BPGM levels in WT and Cse-/- mice with and without GYY4137 treatment. ...View More
Wang, G., Huang, Y., Zhang, N., Liu, W., Wang, C., Zhu, X., & Ni, X. (2021). Hydrogen sulfide is a regulator of hemoglobin oxygen-carrying capacity via controlling 2, 3-BPG production in erythrocytes. Oxidative Medicine and Cellular Longevity, 2021.
CBMAB-2155-YY-2
CBMAB-2155-YY in WB
Interaction of Hb and BPGM with band 4.2 protein. Coimmunoprecipitation with band 4.2 antibody was performed in mouse RBC lysates. BPGM and Hb in the immunoprecipitate were detected by western blot analysis. ...View More
Wang, G., Huang, Y., Zhang, N., Liu, W., Wang, C., Zhu, X., & Ni, X. (2021). Hydrogen sulfide is a regulator of hemoglobin oxygen-carrying capacity via controlling 2, 3-BPG production in erythrocytes. Oxidative Medicine and Cellular Longevity, 2021.
CBMAB-2155-YY-3
CBMAB-2155-YY in IP
Representative images showing the effects of H2S on the interaction of Hb and BPGM with band 4.2 protein. WT and Cse-/- mice were injected with saline or GYY4137 (50 mg/kg). Blood samples were collected 24 h after treatment. Erythrocytes were isolated for the coimmunoprecipitation analysis. ...View More
Wang, G., Huang, Y., Zhang, N., Liu, W., Wang, C., Zhu, X., & Ni, X. (2021). Hydrogen sulfide is a regulator of hemoglobin oxygen-carrying capacity via controlling 2, 3-BPG production in erythrocytes. Oxidative Medicine and Cellular Longevity, 2021.
CBMAB-2155-YY-4
CBMAB-2155-YY in IF
Representative images of BPGM staining in erythrocytes of WT and Cse-/- mice with and without GYY4137 treatment. ...View More
Wang, G., Huang, Y., Zhang, N., Liu, W., Wang, C., Zhu, X., & Ni, X. (2021). Hydrogen sulfide is a regulator of hemoglobin oxygen-carrying capacity via controlling 2, 3-BPG production in erythrocytes. Oxidative Medicine and Cellular Longevity, 2021.

Summary

Host Animal
Mouse
Specificity
Human, Mouse, Rat, Cattle
Clone
CBYY-1806
Antibody Isotype
IgG1, κ
Application
WB, IP, IF, E

Basic Information

Immunogen
Amino acids 153-185 within an internal region of BPGM of human origin.
Host Species
Mouse
Specificity
Human, Mouse, Rat, Cattle
Antibody Isotype
IgG1, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
WB1:100-1:1,000
IP1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)1:50-1:500
ELISA1:100-1:1,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, 0.1% gelatin
Preservative
0.09% sodium azide
Concentration
0.2 mg/ml
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
2,3-bisphosphoglycerate mutase
Introduction
2,3-diphosphoglycerate (2,3-DPG) is a small molecule found at high concentrations in red blood cells where it binds to and decreases the oxygen affinity of hemoglobin. This gene encodes a multifunctional enzyme that catalyzes 2,3-DPG synthesis via its synthetase activity, and 2,3-DPG degradation via its phosphatase activity. The enzyme also has phosphoglycerate phosphomutase activity. Deficiency of this enzyme increases the affinity of cells for oxygen. Mutations in this gene result in hemolytic anemia. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Sep 2009]
Entrez Gene ID
669
UniProt ID
P07738
Alternative Names
Bisphosphoglycerate Mutase; 2,3-Bisphosphoglycerate Mutase, Erythrocyte; 2,3-Bisphosphoglycerate Synthase; 2,3-Diphosphoglycerate Mutase; BPG-Dependent PGAM; DPGM;
Function
Plays a major role in regulating hemoglobin oxygen affinity by controlling the levels of its allosteric effector 2,3-bisphosphoglycerate (2,3-BPG). Also exhibits mutase (EC 5.4.2.11) activity.
Biological Process
Carbohydrate derivative catabolic process Source: Reactome
Carbohydrate metabolic process Source: ProtInc
Erythrocyte development Source: Ensembl
Glycolytic process Source: UniProtKB-KW
Respiratory gaseous exchange by respiratory system Source: ProtInc
Cellular Location
Cytosol; Extracellular exosome
Involvement in disease
Erythrocytosis, familial, 8 (ECYT8): An autosomal recessive disorder characterized by elevated serum hemoglobin and hematocrit, and biphosphoglycerate mutase deficiency. ECYT8 affected individuals manifest hemolytic anemia and splenomegaly.
PTM
Glycation of Lys-159 in diabetic patients inactivates the enzyme.
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For research use only. Not intended for any clinical use.

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