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Mouse Anti-CIITA Recombinant Antibody (CBLC160-LY) (CBMAB-C10987-LY)

See all CIITA antibodies

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Published Data

CBMAB-C10987-LY-1

Mouse Anti-CIITA Recombinant Antibody (CBLC160-LY) (CBMAB-C10987-LY) in WB

Expression of NOD1, NOD2, CIITA, NAIP, NLRP1, NLRP3 and NLRP7 proteins in ovine spleens analyzed by western blot. Note: DN16 = day 16 of the estrous cycle; DP13 = day 13 of pregnancy; DP16 =day 16 of pregnancy; DP25 = day 25 of pregnancy. Significant differences (P < 0.05) are indicated by different superscript letters within the same color column. ...View More

Wu, J., Fang, S., Feng, P., Cai, C., Zhang, L., & Yang, L. (2023). Changes in expression levels of Nod-like receptors in the spleen of ewes. Animal Reproduction, 20(1), e20220093.

Distributed under Open Access license CC BY 4.0, without modification.

CBMAB-C10987-LY-2

Mouse Anti-CIITA Recombinant Antibody (CBLC160-LY) (CBMAB-C10987-LY) in WB

Western blot also revealed that MHCII was upregulated. ...View More

Li, X., Wang, W., Zhang, X., Gong, Z., Tian, M., Zhang, Y., ... & Wu, J. (2022). Neuroinflammation in the medial prefrontal cortex exerts a crucial role in bone cancer pain. Frontiers in Molecular Neuroscience, 15, 1026593.

Distributed under Open Access license CC BY 4.0, without modification.

CBMAB-C10987-LY-3

Mouse Anti-CIITA Recombinant Antibody (CBLC160-LY) (CBMAB-C10987-LY) in WB

PCR products harboring the targeting regions of GGTA1, B2M, and CIITA genes from cell colonies (M, DNA maker DL2000; C, colony). ...View More

Xu, K., Yu, H., Chen, S., Zhang, Y., Guo, J., Yang, C., ... & Wei, H. J. (2022). Production of triple-gene (GGTA1, B2M and CIITA)-modified donor pigs for xenotransplantation. Frontiers in Veterinary Science, 9, 848833.

Distributed under Open Access license CC BY 4.0, without modification.

CBMAB-C10987-LY-4

Mouse Anti-CIITA Recombinant Antibody (CBLC160-LY) (CBMAB-C10987-LY) in IF

Immunofluorometric analysis of B2M and CIITA proteins in the spleen and the liver (scale bar = 20 μm). ...View More

Xu, K., Yu, H., Chen, S., Zhang, Y., Guo, J., Yang, C., ... & Wei, H. J. (2022). Production of triple-gene (GGTA1, B2M and CIITA)-modified donor pigs for xenotransplantation. Frontiers in Veterinary Science, 9, 848833.

Distributed under Open Access license CC BY 4.0, without modification.

CBMAB-C10987-LY-5

Mouse Anti-CIITA Recombinant Antibody (CBLC160-LY) (CBMAB-C10987-LY) in IHC

Immunohistochemical sequential staining for CD163 (red) and CIITA (brown) was performed on eight formalin-fixed paraffin-embedded samples of normal mucosa (NM) and colorectal tumor (CRC) biopsy, respectively. Arrows highlight CD163+ macrophages with low or no expression of CIITA (-/dim). The right plot shows the percentage of macrophages with high (+) or no/low (-/dim) expression of CIITA in normal and CRC tissue expressed as the mean ± SEM. Scale bar, 66 µm. Significance was determined by Student's t-test: * p < 0.05, *** p < 0.001, and **** p < 0.0001. ...View More

Coletta, S., Lonardi, S., Sensi, F., D'Angelo, E., Fassan, M., Pucciarelli, S., ... & Codolo, G. (2021). Tumor cells and the extracellular matrix dictate the pro-tumoral profile of macrophages in CRC. Cancers, 13(20), 5199.

Distributed under Open Access license CC BY 4.0, without modification.

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBLC160-LY

Application

WB, IP, IF, IHC-P

Immunogen

Amino acids 1-350 of purified recombinant CIITA of human.

Host Species

Mouse

Specificity

Mouse, Rat, Human

Antibody Isotype

IgG1, κ

Clonality

Monoclonal Antibody

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)	1:50-1:500
IHC-P	1:50-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, 0.1% gelatin

Preservative

< 0.1% sodium azide

Concentration

0.2 mg/ml

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

Class II Major Histocompatibility Complex Transactivator

Introduction

This gene encodes a protein with an acidic transcriptional activation domain, 4 LRRs (leucine-rich repeats) and a GTP binding domain. The protein is located in the nucleus and acts as a positive regulator of class II major histocompatibility complex gene transcription, and is referred to as the "master control factor" for the expression of these genes. The protein also binds GTP and uses GTP binding to facilitate its own transport into the nucleus. Once in the nucleus it does not bind DNA but rather uses an intrinsic acetyltransferase (AT) activity to act in a coactivator-like fashion. Mutations in this gene have been associated with bare lymphocyte syndrome type II (also known as hereditary MHC class II deficiency or HLA class II-deficient combined immunodeficiency), increased susceptibility to rheumatoid arthritis, multiple sclerosis, and possibly myocardial infarction. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2013]

Entrez Gene ID

Human	4261
Mouse	12265
Rat	85483

UniProt ID

Human	P33076
Mouse	P79621
Rat	G3V6C0

Function

Essential for transcriptional activity of the HLA class II promoter; activation is via the proximal promoter. No DNA binding of in vitro translated CIITA was detected. May act in a coactivator-like fashion through protein-protein interactions by contacting factors binding to the proximal MHC class II promoter, to elements of the transcription machinery, or both. Alternatively it may activate HLA class II transcription by modifying proteins that bind to the MHC class II promoter. Also mediates enhanced MHC class I transcription; the promoter element requirements for CIITA-mediated transcription are distinct from those of constitutive MHC class I transcription, and CIITA can functionally replace TAF1 at these genes. Activates CD74 transcription (PubMed:32855215).

Exhibits intrinsic GTP-stimulated acetyltransferase activity. Exhibits serine/threonine protein kinase activity: can phosphorylate the TFIID component TAF7, the RAP74 subunit of the general transcription factor TFIIF, histone H2B at 'Ser-37' and other histones (in vitro). Has antiviral activity against Ebola virus and coronaviruses, including SARS-CoV-2. Induces resistance by up-regulation of the p41 isoform of CD74, which blocks cathepsin-mediated cleavage of viral glycoproteins, thereby preventing viral fusion (PubMed:32855215).

Biological Process

Immune response Source: ProtInc
Inflammatory response Source: GO_Central
Interferon-gamma-mediated signaling pathway Source: Reactome
Negative regulation of collagen biosynthetic process Source: BHF-UCL
Negative regulation of transcription, DNA-templated Source: BHF-UCL
Negative regulation of transcription by RNA polymerase II Source: BHF-UCL
Negative regulation of viral entry into host cell Source: UniProtKB
Positive regulation of MHC class I biosynthetic process Source: BHF-UCL
Positive regulation of MHC class II biosynthetic process Source: GO_Central
Positive regulation of transcription, DNA-templated Source: BHF-UCL
Positive regulation of transcription by RNA polymerase II Source: UniProtKB
Response to antibiotic Source: MGI
Response to interferon-gamma Source: UniProtKB

Cellular Location

Nucleus; PML body. Recruited to PML body by PML.

Involvement in disease

Bare lymphocyte syndrome 2 (BLS2):
A severe combined immunodeficiency disease with early onset. It is characterized by a profound defect in constitutive and interferon-gamma induced MHC II expression, absence of cellular and humoral T-cell response to antigen challenge, hypogammaglobulinemia and impaired antibody production. The consequence include extreme susceptibility to viral, bacterial and fungal infections.

PTM

Autophosphorylated, affecting interaction with TAF7.

Oda, T., Nakamura, R., Kasamatsu, T., Gotoh, N., Okuda, K., Saitoh, T., ... & Yamashita, T. (2021). DNA-double strand breaks enhance the expression of major histocompatibility complex class II through the ATM-NF-κΒ-IRF1-CIITA pathway. Cancer Gene Therapy, 1-16.

León Machado, J. A., & Steimle, V. (2021). The MHC class II transactivator CIITA: Not (quite) the odd-one-out anymore among NLR proteins. International Journal of Molecular Sciences, 22(3), 1074.

Yotsumoto, K., Sanui, T., Tanaka, U., Yamato, H., Alshargabi, R., Shinjo, T., ... & Nishimura, F. (2020). Amelogenin Downregulates Interferon Gamma-Induced Major Histocompatibility Complex Class II Expression Through Suppression of Euchromatin Formation in the Class II Transactivator Promoter IV Region in Macrophages. Frontiers in immunology, 11, 709.

Bruchez, A., Sha, K., Johnson, J., Chen, L., Stefani, C., McConnell, H., ... & Lacy-Hulbert, A. (2020). MHC class II transactivator CIITA induces cell resistance to Ebola virus and SARS-like coronaviruses. Science, 370(6513), 241-247.

Sandhu, P. K., & Buchkovich, N. J. (2020). Human cytomegalovirus decreases major histocompatibility complex class II by regulating class II transactivator transcript levels in a myeloid cell line. Journal of virology, 94(7), e01901-19.

Anderson III, D. A., Grajales‐Reyes, G. E., Satpathy, A. T., Vasquez Hueichucura, C. E., Murphy, T. L., & Murphy, K. M. (2017). Revisiting the specificity of the MHC class II transactivator CIITA in classical murine dendritic cells in vivo. European journal of immunology, 47(8), 1317-1323.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

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