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Mouse Anti-CIITA Recombinant Antibody (CBWJC-2715) (CBMAB-C3810WJ)

This product is a Mouse antibody that recognizes CIITA. This antibody CBWJC-2715 can be used for immunoassay techniques such as: WB, IP, IF, ELISA.
See all CIITA antibodies

Summary

Host Animal
Mouse
Specificity
Human
Clone
CBWJC-2715
Antibody Isotype
IgG
Application
WB, IP, IF, ELISA

Basic Information

Specificity
Human
Antibody Isotype
IgG
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
Class II Major Histocompatibility Complex Transactivator
Introduction
This gene encodes a protein with an acidic transcriptional activation domain, 4 LRRs (leucine-rich repeats) and a GTP binding domain. The protein is located in the nucleus and acts as a positive regulator of class II major histocompatibility complex gene transcription, and is referred to as the "master control factor" for the expression of these genes. The protein also binds GTP and uses GTP binding to facilitate its own transport into the nucleus. Once in the nucleus it does not bind DNA but rather uses an intrinsic acetyltransferase (AT) activity to act in a coactivator-like fashion. Mutations in this gene have been associated with bare lymphocyte syndrome type II (also known as hereditary MHC class II deficiency or HLA class II-deficient combined immunodeficiency), increased susceptibility to rheumatoid arthritis, multiple sclerosis, and possibly myocardial infarction. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2013]
Entrez Gene ID
4261
UniProt ID
P33076
Alternative Names
Class II Major Histocompatibility Complex Transactivator; Nucleotide-Binding Oligomerization Domain, Leucine Rich Repeat And Acid Domain Containing; NLR Family, Acid Domain Containing; MHC Class II Transactivator; MHC2TA; Class II, Major Histocompatibility Complex, Transactivator; MHC Class II Transactivator Type III;
Function
Essential for transcriptional activity of the HLA class II promoter; activation is via the proximal promoter. No DNA binding of in vitro translated CIITA was detected. May act in a coactivator-like fashion through protein-protein interactions by contacting factors binding to the proximal MHC class II promoter, to elements of the transcription machinery, or both. Alternatively it may activate HLA class II transcription by modifying proteins that bind to the MHC class II promoter. Also mediates enhanced MHC class I transcription; the promoter element requirements for CIITA-mediated transcription are distinct from those of constitutive MHC class I transcription, and CIITA can functionally replace TAF1 at these genes. Activates CD74 transcription (PubMed:32855215).

Exhibits intrinsic GTP-stimulated acetyltransferase activity. Exhibits serine/threonine protein kinase activity: can phosphorylate the TFIID component TAF7, the RAP74 subunit of the general transcription factor TFIIF, histone H2B at 'Ser-37' and other histones (in vitro). Has antiviral activity against Ebola virus and coronaviruses, including SARS-CoV-2. Induces resistance by up-regulation of the p41 isoform of CD74, which blocks cathepsin-mediated cleavage of viral glycoproteins, thereby preventing viral fusion (PubMed:32855215).
Biological Process
Immune response Source: ProtInc
Inflammatory response Source: GO_Central
Interferon-gamma-mediated signaling pathway Source: Reactome
Negative regulation of collagen biosynthetic process Source: BHF-UCL
Negative regulation of transcription, DNA-templated Source: BHF-UCL
Negative regulation of transcription by RNA polymerase II Source: BHF-UCL
Negative regulation of viral entry into host cell Source: UniProtKB
Positive regulation of MHC class I biosynthetic process Source: BHF-UCL
Positive regulation of MHC class II biosynthetic process Source: GO_Central
Positive regulation of transcription, DNA-templated Source: BHF-UCL
Positive regulation of transcription by RNA polymerase II Source: UniProtKB
Response to antibiotic Source: MGI
Response to interferon-gamma Source: UniProtKB
Cellular Location
Nucleus; PML body. Recruited to PML body by PML.
Involvement in disease
Bare lymphocyte syndrome 2 (BLS2):
A severe combined immunodeficiency disease with early onset. It is characterized by a profound defect in constitutive and interferon-gamma induced MHC II expression, absence of cellular and humoral T-cell response to antigen challenge, hypogammaglobulinemia and impaired antibody production. The consequence include extreme susceptibility to viral, bacterial and fungal infections.
PTM
Autophosphorylated, affecting interaction with TAF7.

Oda, T., Nakamura, R., Kasamatsu, T., Gotoh, N., Okuda, K., Saitoh, T., ... & Yamashita, T. (2021). DNA-double strand breaks enhance the expression of major histocompatibility complex class II through the ATM-NF-κΒ-IRF1-CIITA pathway. Cancer Gene Therapy, 1-16.

León Machado, J. A., & Steimle, V. (2021). The MHC class II transactivator CIITA: Not (quite) the odd-one-out anymore among NLR proteins. International Journal of Molecular Sciences, 22(3), 1074.

Yotsumoto, K., Sanui, T., Tanaka, U., Yamato, H., Alshargabi, R., Shinjo, T., ... & Nishimura, F. (2020). Amelogenin Downregulates Interferon Gamma-Induced Major Histocompatibility Complex Class II Expression Through Suppression of Euchromatin Formation in the Class II Transactivator Promoter IV Region in Macrophages. Frontiers in immunology, 11, 709.

Bruchez, A., Sha, K., Johnson, J., Chen, L., Stefani, C., McConnell, H., ... & Lacy-Hulbert, A. (2020). MHC class II transactivator CIITA induces cell resistance to Ebola virus and SARS-like coronaviruses. Science, 370(6513), 241-247.

Sandhu, P. K., & Buchkovich, N. J. (2020). Human cytomegalovirus decreases major histocompatibility complex class II by regulating class II transactivator transcript levels in a myeloid cell line. Journal of virology, 94(7), e01901-19.

Anderson III, D. A., Grajales‐Reyes, G. E., Satpathy, A. T., Vasquez Hueichucura, C. E., Murphy, T. L., & Murphy, K. M. (2017). Revisiting the specificity of the MHC class II transactivator CIITA in classical murine dendritic cells in vivo. European journal of immunology, 47(8), 1317-1323.

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For research use only. Not intended for any clinical use.

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