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Mouse Anti-NUDT5 Recombinant Antibody (E-4) (CBMAB-N3909-WJ)

This product is a Mouse antibody that recognizes NUDT5. The antibody E-4 can be used for immunoassay techniques such as: WB, IP, IF, ELISA.
See all NUDT5 antibodies

Summary

Host Animal
Mouse
Specificity
Mouse, Rat, Human
Clone
E-4
Application
WB, IP, IF, ELISA

Basic Information

Specificity
Mouse, Rat, Human
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
nudix (nucleoside diphosphate linked moiety X)-type motif 5
Introduction
This gene belongs to the Nudix (nucleoside diphosphate linked moiety X) hydrolase superfamily. The encoded enzyme catalyzes the hydrolysis of modified nucleoside diphosphates, including ADP-ribose (ADPR) and 8-oxoGua-containing 8-oxo-dADP and 8-oxo-dGDP. Protein-bound ADP ribose can be hazardous to the cell because it can modify some amino acid residues, resulting in the inhibition of ATP-activated potassium channels. 8-oxoGua is an oxidized form of guanine that can potentially alter genetic information by pairing with adenine and cytosine in RNA. Presence of 8-oxoGua in RNA results in formation of abnormal proteins due to translational errors. [provided by RefSeq, Aug 2013]
Entrez Gene ID
Human11164
Mouse53893
Rat361274
UniProt ID
HumanQ9UKK9
MouseQ9JKX6
RatQ6AY63
Alternative Names
Nudix Hydrolase 5; Nudix (Nucleoside Diphosphate Linked Moiety X)-Type Motif 5; Nuclear ATP-Synthesis Protein NUDIX5; 8-Oxo-DGDP Phosphatase; HNUDT5; YSA1H; Nucleoside Diphosphate-Linked Moiety X Motif 5; ADP-Sugar Pyrophosphatase;
Function
Enzyme that can either act as an ADP-sugar pyrophosphatase in absence of diphosphate or catalyze the synthesis of ATP in presence of diphosphate (PubMed:27257257).
In absence of diphosphate, hydrolyzes with similar activities various modified nucleoside diphosphates such as ADP-ribose, ADP-mannose, ADP-glucose, 8-oxo-GDP and 8-oxo-dGDP (PubMed:10567213, PubMed:10722730, PubMed:19699693, PubMed:21389046, PubMed:17052728).
Can also hydrolyze other nucleotide sugars with low activity (PubMed:19699693, PubMed:21389046).
In presence of diphosphate, mediates the synthesis of ATP in the nucleus by catalyzing the conversion of ADP-ribose to ATP and ribose 5-phosphate. Nuclear ATP synthesis takes place when dephosphorylated at Thr-45 (PubMed:27257257).
Nuclear ATP generation is required for extensive chromatin remodeling events that are energy-consuming (PubMed:27257257).
Does not play a role in U8 snoRNA decapping activity (By similarity).
Binds U8 snoRNA (By similarity).
Biological Process
ATP generation from poly-ADP-D-riboseManual Assertion Based On ExperimentIDA:UniProtKB
Chromatin remodelingManual Assertion Based On ExperimentIDA:UniProtKB
D-ribose catabolic processManual Assertion Based On ExperimentIDA:UniProtKB
Nucleobase-containing small molecule metabolic processTAS:Reactome
Nucleoside phosphate metabolic processManual Assertion Based On ExperimentIBA:GO_Central
Nucleotide metabolic process1 PublicationNAS:ProtInc
Ribonucleoside diphosphate catabolic processManual Assertion Based On ExperimentIDA:UniProtKB
Ribose phosphate metabolic processManual Assertion Based On ExperimentIBA:GO_Central
Cellular Location
Nucleus
PTM
Phosphorylation at Thr-45 is required for homodimer stability; dephosphorylation results in destabilization of the homodimer. Dephosphorylation at Thr-45 promotes the ATP-synthesis activity.

Qian, J., Ma, Y., Tahaney, W. M., Moyer, C. L., Lanier, A., Hill, J., ... & Brown, P. H. (2024). The novel phosphatase NUDT5 is a critical regulator of triple-negative breast cancer growth. Breast Cancer Research, 26(1), 23.

Ding, H., Wu, C., Sun, W., Zhan, Q., Huang, Y., Liao, N., ... & Li, Y. (2023). NUDT5-Determines the fate of head and neck squamous cell carcinoma cells under endoplasmic reticulum stress by catalyzing nuclear ATP production to promote DNA repair. Oral Oncology, 141, 106397.

Niranjan, V., Jayaprasad, S., Uttarkar, A., Kusanur, R., & Kumar, J. (2022). Design of novel coumarin derivatives as NUDT5 antagonists that act by restricting ATP synthesis in breast cancer cells. Molecules, 28(1), 89.

Qi, H., Wright, R. H. G., Beato, M., & Price, B. D. (2022). The ADP-ribose hydrolase NUDT5 is important for DNA repair. Cell Reports, 41(12).

Li, D. N., Yang, C. C., Li, J., Yang, Q. G. O., Zeng, L. T., Fan, G. Q., ... & Cai, J. P. (2021). The high expression of MTH1 and NUDT5 promotes tumor metastasis and indicates a poor prognosis in patients with non-small-cell lung cancer. Biochimica et Biophysica Acta (BBA)-Molecular Cell Research, 1868(1), 118895.

Tong, X. Y., Quan, Y., & Zhang, H. Y. (2021). NUDT5 as a novel drug target and prognostic biomarker for ER-positive breast cancer. Drug Discovery Today, 26(3), 620-625.

Zhang, H., Zhang, L. Q., Yang, C. C., Li, J., Tian, X. Y., Li, D. N., ... & Cai, J. P. (2021). The high expression of NUDT5 indicates poor prognosis of breast cancer by modulating AKT/Cyclin D signaling. PLoS One, 16(2), e0245876.

Tong, X. Y., Liao, X., Gao, M., Lv, B. M., Chen, X. H., Chu, X. Y., ... & Zhang, H. Y. (2020). Identification of NUDT5 inhibitors from approved drugs. Frontiers in Molecular Biosciences, 7, 44.

Wang, J. J., Liu, T. H., Li, J., Li, D. N., Tian, X. Y., Ouyang, Q. G., & Cai, J. P. (2020). The high expression of MTH1 and NUDT5 predict a poor survival and are associated with malignancy of esophageal squamous cell carcinoma. PeerJ, 8, e9195.

Pickup, K. E., Pardow, F., Carbonell-Caballero, J., Lioutas, A., Villanueva-Cañas, J. L., Wright, R. H., & Beato, M. (2019). Expression of oncogenic drivers in 3D cell culture depends on nuclear ATP synthesis by NUDT5. Cancers, 11(9), 1337.

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For research use only. Not intended for any clinical use.

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