Mouse Anti-NUF2 Recombinant Antibody (CBWJN-1607) (CBMAB-N3930-WJ)

This product is a Mouse antibody that recognizes NUF2. The antibody CBWJN-1607 can be used for immunoassay techniques such as: WB, IP.
See all NUF2 antibodies

Datasheet

Summary

Host Animal

Mouse

Specificity

Human

Clone

CBWJN-1607

Antibody Isotype

IgG1

Application

WB, IP

Basic Information

Specificity

Human

Antibody Isotype

IgG1

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Concentration

1 mg/mL

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name

NUF2, NDC80 Kinetochore Complex Component

Introduction

This gene encodes a protein that is highly similar to yeast Nuf2, a component of a conserved protein complex associated with the centromere. Yeast Nuf2 disappears from the centromere during meiotic prophase when centromeres lose their connection to the spindle pole body, and plays a regulatory role in chromosome segregation. The encoded protein is found to be associated with centromeres of mitotic HeLa cells, which suggests that this protein is a functional homolog of yeast Nuf2. Alternatively spliced transcript variants that encode the same protein have been described. [provided by RefSeq, Jul 2008]

Entrez Gene ID

83540

UniProt ID

Q9BZD4

Alternative Names

NUF2, NDC80 Kinetochore Complex Component; Cell Division Cycle-Associated Protein 1; Cell Division Cycle Associated 1; Cancer/Testis Antigen 106; HNuf2R; HsNuf2; CDCA1;

Function

Acts as a component of the essential kinetochore-associated NDC80 complex, which is required for chromosome segregation and spindle checkpoint activity (PubMed:12438418, PubMed:14654001, PubMed:15062103, PubMed:15235793, PubMed:15239953, PubMed:15548592, PubMed:17535814).
Required for kinetochore integrity and the organization of stable microtubule binding sites in the outer plate of the kinetochore (PubMed:15548592).
The NDC80 complex synergistically enhances the affinity of the SKA1 complex for microtubules and may allow the NDC80 complex to track depolymerizing microtubules (PubMed:23085020).

Biological Process

Attachment of mitotic spindle microtubules to kinetochoreManual Assertion Based On ExperimentIBA:GO_Central
Attachment of spindle microtubules to kinetochoreManual Assertion Based On ExperimentIDA:ComplexPortal
Cell divisionIEA:UniProtKB-KW
Chromosome segregation1 PublicationNAS:UniProtKB
Kinetochore organizationManual Assertion Based On ExperimentIBA:GO_Central
Meiotic chromosome segregationManual Assertion Based On ExperimentIBA:GO_Central
Mitotic spindle assembly checkpoint signaling1 PublicationIC:ComplexPortal
Mitotic spindle organizationManual Assertion Based On ExperimentIBA:GO_Central

Cellular Location

Nucleus
Chromosome, centromere, kinetochore
Localizes to kinetochores from late prophase to anaphase. Localizes specifically to the outer plate of the kinetochore. NDC80 is required for efficient kinetochore localization.

PTM

Can be phosphorylated by AURKA and AURKB.

References

Polley, S., Müschenborn, H., Terbeck, M., De Antoni, A., Vetter, I. R., Dogterom, M., ... & Huis In't Veld, P. J. (2023). Stable kinetochore‐microtubule attachment requires loop‐dependent Ndc80‐Ndc80 binding. The EMBO Journal, 42(13), e112504.

Amin, M. A., Wallace, D. A., & Varma, D. (2022). The Ndc80 complex is essential for the initial kinetochore-microtubule capture during early mitosis. bioRxiv, 2022-02.

Uehara, D. T., Mitsubuchi, H., & Inazawa, J. (2021). A missense variant in NUF2, a component of the kinetochore NDC80 complex, causes impaired chromosome segregation and aneuploidy associated with microcephaly and short stature. Human Genetics, 140(7), 1047-1060.

Ustinov, N. B., Korshunova, A. V., & Gudimchuk, N. B. (2020). Protein complex NDC80: Properties, functions, and possible role in pathophysiology of cell division. Biochemistry (Moscow), 85, 448-462.

Zhao, G., Cheng, Y., Gui, P., Cui, M., Liu, W., Wang, W., ... & Yao, X. (2019). Dynamic acetylation of the kinetochore-associated protein HEC1 ensures accurate microtubule–kinetochore attachment. Journal of Biological Chemistry, 294(2), 576-592.

Brusini, L., D’Archivio, S., McDonald, J., & Wickstead, B. (2019). Ndc80/Nuf2-like protein KKIP1 connects a stable kinetoplastid outer kinetochore complex to the inner kinetochore and responds to metaphase tension. BioRxiv, 764829.

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Protocols

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Associated Products

Related Products

Mouse Anti-NUF2 Recombinant Antibody (CBWJN-0330)

Mouse Anti-NUF2 Recombinant Antibody (CBWJN-1608)

Rabbit Anti-NUF2 Recombinant Antibody (CBWJN-1609)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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