Mouse Anti-NXF1 Recombinant Antibody (53H8) (CBMAB-N4054-WJ)

This product is a Mouse antibody that recognizes NXF1. The antibody 53H8 can be used for immunoassay techniques such as: ELISA, IF, IP, WB.
See all NXF1 antibodies

Published Data

Fig.1 Total extracts from N2A cells transfected with either FLAG control (FLAG ctrl) and either FLAG-tagged SRSF1 aa11-196 wild-type (SRSF1), SRSF1-m2 or SRSF1-m4 are subjected to anti-FLAG immunoprecipitation. Co-immunoprecipitation of endogenous NXF1 is assessed using α-NXF1 antibodies.

Fig.2 Immunofluorescence images of HeLa cells transfected with the indicated expression vectors. FLAG-Nxf1 was detected using FLAG antibody.

Datasheet

Summary

Host Animal

Mouse

Specificity

Human

Clone

53H8

Antibody Isotype

IgG1

Application

ELISA, IF, IP, WB

Basic Information

Specificity

Human

Antibody Isotype

IgG1

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS

Preservative

0.1% sodium azide

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name

Nuclear RNA Export Factor 1

Introduction

This gene is one member of a family of nuclear RNA export factor genes. Common domain features of this family are a noncanonical RNP-type RNA-binding domain (RBD), 4 leucine-rich repeats (LRRs), a nuclear transport factor 2 (NTF2)-like domain that allows heterodimerization with NTF2-related export protein-1 (NXT1), and a ubiquitin-associated domain that mediates interactions with nucleoporins. The LRRs and NTF2-like domains are required for export activity. Alternative splicing seems to be a common mechanism in this gene family. The encoded protein of this gene shuttles between the nucleus and the cytoplasm and binds in vivo to poly(A)+ RNA. It is the vertebrate homologue of the yeast protein Mex67p. The encoded protein overcomes the mRNA export block caused by the presence of saturating amounts of CTE (constitutive transport element) RNA of type D retroviruses. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2008]

Entrez Gene ID

10482

UniProt ID

Q9UBU9

Alternative Names

Nuclear RNA Export Factor 1; Tip Associating Protein; Tip-Associated Protein; MRNA Export Factor TAP; TAP; Tip-Associating Protein; MEX67;

Function

Involved in the nuclear export of mRNA species bearing retroviral constitutive transport elements (CTE) and in the export of mRNA from the nucleus to the cytoplasm (TAP/NFX1 pathway) (PubMed:10924507).
The NXF1-NXT1 heterodimer is involved in the export of HSP70 mRNA in conjunction with ALYREF/THOC4 and THOC5 components of the TREX complex (PubMed:18364396, PubMed:19165146, PubMed:9660949).
ALYREF/THOC4-bound mRNA is thought to be transferred to the NXF1-NXT1 heterodimer for export (PubMed:18364396, PubMed:19165146, PubMed:9660949).
Also involved in nuclear export of m6A-containing mRNAs: interaction between SRSF3 and YTHDC1 facilitates m6A-containing mRNA-binding to both SRSF3 and NXF1, promoting mRNA nuclear export (PubMed:28984244).

Biological Process

mRNA export from nucleusManual Assertion Based On ExperimentIDA:UniProtKB
Poly(A)+ mRNA export from nucleusManual Assertion Based On ExperimentIBA:GO_Central
Protein transportIEA:UniProtKB-KW

Cellular Location

Nucleus
Nucleus, nucleoplasm
Nucleus speckle
Nucleus, nuclear pore complex
Nucleus envelope
Cytoplasm
Cytoplasm, Stress granule
Localized predominantly in the nucleoplasm and at both the nucleoplasmic and cytoplasmic faces of the nuclear pore complex. Shuttles between the nucleus and the cytoplasm. Travels to the cytoplasm as part of the exon junction complex (EJC) bound to mRNA. The association with the TREX complex seems to occur in regions surrounding nuclear speckles known as perispeckles (PubMed:23826332).
Nucleus; nuclear rim (PubMed:25662211).

References

Wendt, L., Brandt, J., Ushakov, D. S., Bodmer, B. S., Pickin, M. J., Groseth, A., & Hoenen, T. (2022). Evidence for viral mRNA export from Ebola virus inclusion bodies by the nuclear RNA export factor NXF1. Journal of Virology, 96(18), e00900-22.

Mougel, M., Akkawi, C., Chamontin, C., Feuillard, J., Pessel-Vivares, L., Socol, M., & Laine, S. (2020). NXF1 and CRM1 nuclear export pathways orchestrate nuclear export, translation and packaging of murine leukaemia retrovirus unspliced RNA. RNA biology, 17(4), 528-538.

Zuckerman, B., Ron, M., Mikl, M., Segal, E., & Ulitsky, I. (2020). Gene architecture and sequence composition underpin selective dependency of nuclear export of long RNAs on NXF1 and the TREX complex. Molecular Cell, 79(2), 251-267.

Wendt, L., Brandt, J., Bodmer, B. S., Reiche, S., Schmidt, M. L., Traeger, S., & Hoenen, T. (2020). The Ebola virus nucleoprotein recruits the nuclear RNA export factor NXF1 into inclusion bodies to facilitate viral protein expression. Cells, 9(1), 187.

Chen, S., Wang, R., Zheng, D., Zhang, H., Chang, X., Wang, K., ... & Cheng, H. (2019). The mRNA export receptor NXF1 coordinates transcriptional dynamics, alternative polyadenylation, and mRNA export. Molecular cell, 74(1), 118-131.

Murano, K., Iwasaki, Y. W., Ishizu, H., Mashiko, A., Shibuya, A., Kondo, S., ... & Siomi, H. (2019). Nuclear RNA export factor variant initiates piRNA‐guided co‐transcriptional silencing. The EMBO journal, 38(17), e102870.

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Protocols

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Associated Products

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Isotype control

For research use only. Not intended for any clinical use.

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