Rabbit Anti-NXF1 Recombinant Antibody (D5X4G) (CBMAB-N4055-WJ)

This product is a Rabbit antibody that recognizes NXF1. The antibody D5X4G can be used for immunoassay techniques such as: WB.
See all NXF1 antibodies

Datasheet

Summary

Host Animal

Rabbit

Specificity

Human, Mouse, Rat, Monkey, Hamster, Cattle, Dog, Pig, Guinea pig

Clone

D5X4G

Application

Basic Information

Specificity

Human, Mouse, Rat, Monkey, Hamster, Cattle, Dog, Pig, Guinea pig

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name

Nuclear RNA Export Factor 1

Introduction

This gene is one member of a family of nuclear RNA export factor genes. Common domain features of this family are a noncanonical RNP-type RNA-binding domain (RBD), 4 leucine-rich repeats (LRRs), a nuclear transport factor 2 (NTF2)-like domain that allows heterodimerization with NTF2-related export protein-1 (NXT1), and a ubiquitin-associated domain that mediates interactions with nucleoporins. The LRRs and NTF2-like domains are required for export activity. Alternative splicing seems to be a common mechanism in this gene family. The encoded protein of this gene shuttles between the nucleus and the cytoplasm and binds in vivo to poly(A)+ RNA. It is the vertebrate homologue of the yeast protein Mex67p. The encoded protein overcomes the mRNA export block caused by the presence of saturating amounts of CTE (constitutive transport element) RNA of type D retroviruses. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2008]

Entrez Gene ID

Human	10482
Mouse	53319
Rat	59087
Hamster	101827368
Monkey	718420
Cattle	512136
Dog	483780
Pig	100628213
Guinea pig	100728879

UniProt ID

Human	Q9UBU9
Mouse	Q99JX7
Rat	O88984
Hamster	A0A061IF78
Monkey	H9YW98
Cattle	Q1RMS5
Dog	F6XRT0
Pig	I3LNX4
Guinea pig	H0V731

Alternative Names

Nuclear RNA Export Factor 1; Tip Associating Protein; Tip-Associated Protein; MRNA Export Factor TAP; TAP; Tip-Associating Protein; MEX67;

Function

Involved in the nuclear export of mRNA species bearing retroviral constitutive transport elements (CTE) and in the export of mRNA from the nucleus to the cytoplasm (TAP/NFX1 pathway) (PubMed:10924507).
The NXF1-NXT1 heterodimer is involved in the export of HSP70 mRNA in conjunction with ALYREF/THOC4 and THOC5 components of the TREX complex (PubMed:18364396, PubMed:19165146, PubMed:9660949).
ALYREF/THOC4-bound mRNA is thought to be transferred to the NXF1-NXT1 heterodimer for export (PubMed:18364396, PubMed:19165146, PubMed:9660949).
Also involved in nuclear export of m6A-containing mRNAs: interaction between SRSF3 and YTHDC1 facilitates m6A-containing mRNA-binding to both SRSF3 and NXF1, promoting mRNA nuclear export (PubMed:28984244).

Biological Process

mRNA export from nucleusManual Assertion Based On ExperimentIDA:UniProtKB
Poly(A)+ mRNA export from nucleusManual Assertion Based On ExperimentIBA:GO_Central
Protein transportIEA:UniProtKB-KW

Cellular Location

Nucleus
Nucleus, nucleoplasm
Nucleus speckle
Nucleus, nuclear pore complex
Nucleus envelope
Cytoplasm
Cytoplasm, Stress granule
Localized predominantly in the nucleoplasm and at both the nucleoplasmic and cytoplasmic faces of the nuclear pore complex. Shuttles between the nucleus and the cytoplasm. Travels to the cytoplasm as part of the exon junction complex (EJC) bound to mRNA. The association with the TREX complex seems to occur in regions surrounding nuclear speckles known as perispeckles (PubMed:23826332).
Nucleus; nuclear rim (PubMed:25662211).

References

Wendt, L., Brandt, J., Ushakov, D. S., Bodmer, B. S., Pickin, M. J., Groseth, A., & Hoenen, T. (2022). Evidence for viral mRNA export from Ebola virus inclusion bodies by the nuclear RNA export factor NXF1. Journal of Virology, 96(18), e00900-22.

Mougel, M., Akkawi, C., Chamontin, C., Feuillard, J., Pessel-Vivares, L., Socol, M., & Laine, S. (2020). NXF1 and CRM1 nuclear export pathways orchestrate nuclear export, translation and packaging of murine leukaemia retrovirus unspliced RNA. RNA biology, 17(4), 528-538.

Zuckerman, B., Ron, M., Mikl, M., Segal, E., & Ulitsky, I. (2020). Gene architecture and sequence composition underpin selective dependency of nuclear export of long RNAs on NXF1 and the TREX complex. Molecular Cell, 79(2), 251-267.

Wendt, L., Brandt, J., Bodmer, B. S., Reiche, S., Schmidt, M. L., Traeger, S., & Hoenen, T. (2020). The Ebola virus nucleoprotein recruits the nuclear RNA export factor NXF1 into inclusion bodies to facilitate viral protein expression. Cells, 9(1), 187.

Chen, S., Wang, R., Zheng, D., Zhang, H., Chang, X., Wang, K., ... & Cheng, H. (2019). The mRNA export receptor NXF1 coordinates transcriptional dynamics, alternative polyadenylation, and mRNA export. Molecular cell, 74(1), 118-131.

Murano, K., Iwasaki, Y. W., Ishizu, H., Mashiko, A., Shibuya, A., Kondo, S., ... & Siomi, H. (2019). Nuclear RNA export factor variant initiates piRNA‐guided co‐transcriptional silencing. The EMBO journal, 38(17), e102870.

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Protocols

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Associated Products

Related Products

Mouse Anti-NXF1 Recombinant Antibody (53H8)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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