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Human Immune Checkpoint Antibody Array (Glass Slide, 10 Targets) (Y0181) (AbAr-0181-YC)

Antibody array is a specific form of protein microarray. In this technology, capture antibodies spotted on membranes or glass slide bind to specific target proteins present in the sample. Captured proteins are visualized using chemiluminescent or Laser Scanner. The signal produced is proportional to the amount of analyte bound. The Human Immune Checkpoint Molecule Antibody Array detects 10 Human Immune Checkpoint Molecules. It is suitable for all liquid sample types.
Target Array
Immune Checkpoint Molecule Array
B7-H2 (ICOS Ligand), CTLA-4 (CD152), B7-H3 (CD276), ICOS, CD28, PD-1, CD80 (B7-1), PD-L1 (B7-H1/CD274), CD86 (B7-2), PD-L2 (B7-DC)
Species Reactivity
Sample Type
Serum, Plasma, Cell Culture Supernatant, Cell Lysates, Tissue Lysates, Other Body Fluids
Multiplex Protein Detection
Assay Type
Solid Support
Glass Slide
Detection Assay
Protocol Outline
Dry the glass slide
Prepare Standards
Block array surface
Incubate with Samples and Standards
Incubate with Biotinylated Detection Antibody Cocktail
Incubate with Streptavidin-Conjugated Fluor
Disassemble the glass slide
Scan with a gene microarray laser scanner
Perform densitometry and analysis
Antibody Array Slide
Labeling Reagent
Blocking Buffer
Wash Buffer
Detection Buffer
Detection Cocktail
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Once thawed, please keep reagents under suitable conditions respectively. Do not use past expiration date.
Immune checkpoint is a kind of signal for regulating the antigen recognition of T cell receptor (TCR) in the process of immune responce. Immune checkpoint contains two kinds of signals: (1) Co-stimulatory immune checkpoint: stimulating immune progress, such as CD28, ICOS, and CD137; (2) Co-inhibitory immune checkpoint: inhibiting immune progress, such as PD1, CTLA-4, and VISTA. When immune system is attacking pathogens, these immune checkpoint molecules can protect the normal tissues from damage. The cancer cells cleverly escape from immune attack by dysregulating immune checkpoint related proteins. Immune checkpoint therapy relys on functioning immune system with agonists of co-stimulatory signals or antagonists of inhibitory signals.
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For Research Use Only. Not For Clinical Use.