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Rat Immune Checkpoint Antibody Array (Membrane, 9 Targets) (Y0542) (AbAr-0542-YC)

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Specifications

Target Array
Immune Checkpoint Array
Target
4-1BB, CD27, CD80, CD86, LGALS1, LGALS3, Fas, Fas Ligand, TIM-1
Species Reactivity
Rat
Sample Type
Cell culture supernatants, Cell lysates, Plasma, Serum and Tissue lysates

Applications

Application
Multiplexed Protein Detection
Assay Type
Semi-Quantitative
Solid Support
Membrane
Detection Assay
Sandwich-based Assay
Protocol Outline
Conduct blocking of the array membrane surface.
Proceed with incubation of the sample.
Incubate with a biotinylated detection antibody cocktail.
Continue with incubation using HRP-streptavidin.
Engage in incubation with detection buffers.
Capture images using a chemiluminescent imaging system.
Perform data analysis.
Components
Antibody Array Membrane
Labeling Reagent
Blocking Buffer
Washing Buffer
Detection Buffer
Detection Cocktail
Others
Storage
Store at -20°C for 6 months. Once thawed, please keep reagents under suitable conditions respectively for no more than 3 months. Do not use past expiration date.

Background

Introduction
Immune checkpoint is a kind of signal for regulating the antigen recognition of T cell receptor (TCR) in the process of immune responce. Immune checkpoint contains two kinds of signals: (1) Co-stimulatory immune checkpoint: stimulating immune progress, such as CD28, ICOS, and CD137; (2) Co-inhibitory immune checkpoint: inhibiting immune progress, such as PD1, CTLA-4, and VISTA. When immune system is attacking pathogens, these immune checkpoint molecules can protect the normal tissues from damage. The cancer cells cleverly escape from immune attack by dysregulating immune checkpoint related proteins. Immune checkpoint therapy relys on functioning immune system with agonists of co-stimulatory signals or antagonists of inhibitory signals.
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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

Alternative Versions

Human Immune Checkpoint Antibody Array (Membrane, 23 Targets) (Y0212) (CAT#: AbAr-0212-YC)

Human Immune Checkpoint Antibody Array (Membrane, 11 Targets) (Y0213) (CAT#: AbAr-0213-YC)

Human Immune Checkpoint Antibody Array (Membrane, 12 Targets) (Y0214) (CAT#: AbAr-0214-YC)

Mouse Immune Checkpoint Antibody Array (Membrane, 11 Targets) (Y0215) (CAT#: AbAr-0215-YC)

Human Immune Checkpoint Antibody Array (Glass Slide, 10 Targets) (Y0181) (CAT#: AbAr-0181-YC)

Human Immune Checkpoint Antibody Array (Glass Slide, 10 Targets) (Y0543) (CAT#: AbAr-0543-YC)

Antibody Array

Human Immune Checkpoint Antibody Array (Membrane, 23 Targets) (Y0212) (CAT#: AbAr-0212-YC)

Human Immune Checkpoint Antibody Array (Membrane, 11 Targets) (Y0213) (CAT#: AbAr-0213-YC)

Human Immune Checkpoint Antibody Array (Membrane, 12 Targets) (Y0214) (CAT#: AbAr-0214-YC)

Mouse Immune Checkpoint Antibody Array (Membrane, 11 Targets) (Y0215) (CAT#: AbAr-0215-YC)

Human Immune Checkpoint Antibody Array (Glass Slide, 10 Targets) (Y0181) (CAT#: AbAr-0181-YC)

Human Immune Checkpoint Antibody Array (Glass Slide, 10 Targets) (Y0543) (CAT#: AbAr-0543-YC)

Related Products

Human Immune Checkpoint Antibody Array (Membrane, 11 Targets) (Y0213) (CAT#: AbAr-0213-YC)

Human Immune Checkpoint Antibody Array (Glass Slide, 10 Targets) (Y0543) (CAT#: AbAr-0543-YC)

Human Immune Checkpoint Antibody Array (Membrane, 12 Targets) (Y0214) (CAT#: AbAr-0214-YC)

Human Immune Checkpoint Antibody Array (Glass Slide, 10 Targets) (Y0181) (CAT#: AbAr-0181-YC)

Mouse Immune Checkpoint Antibody Array (Membrane, 11 Targets) (Y0215) (CAT#: AbAr-0215-YC)

Human Immune Checkpoint Antibody Array (Membrane, 23 Targets) (Y0212) (CAT#: AbAr-0212-YC)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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