Sign in or Register   Sign in or Register
  |  

Rat Immune Checkpoint Antibody Array (Membrane, 9 Targets) (Y0542) (AbAr-0542-YC)

Antibody array is a specific form of protein microarray. In this technology, capture antibodies spotted on membranes or glass slide bind to specific target proteins present in the sample. Captured proteins are visualized using chemiluminescent or Laser Scanner. The signal produced is proportional to the amount of analyte bound. The product provided herein is a Rat Immune Checkpoint Antibody Array, which can be used for semi-quantitative detection of 9 rat immune checkpoint molecules. It is suitable for a range of liquid forms including Cell culture supernatants, Cell lysates, Plasma, Serum and Tissue lysates.
See all Immune Checkpoint antibodies

Specifications

Target Array
Immune Checkpoint Array
Target
4-1BB(TNFRSF9/CD137), CD27(TNFRSF7), CD80(B7-1), CD86(B7-2), Galectin-1, Galectin-3, Fas(TNFRSF6/Apo-1), Fas Ligand(TNFSF6), TIM-1(KIM-1)
Species Reactivity
Rat
Sample Type
Cell culture supernatants, Cell lysates, Plasma, Serum and Tissue lysates

Applications

Application
Multiplexed Protein Detection
Assay Type
Semi-Quantitative
Solid Support
Membrane
Detection Assay
Sandwich-based Assay
Protocol Outline
Block the array membrane surface
Incubate with Sample
Incubate with Biotinylated Detection Antibody Cocktail
Incubate with HRP Conjugated Streptavidin
Incubate with Detection Buffers
Image with chemiluminescent imaging system
Analyze data
Components
Antibody Array Membrane
Labeling Reagent
Blocking Buffer
Washing Buffer
Detection Buffer
Detection Cocktail
Others
Storage
Store at -20°C for 6 months. Once thawed, please keep reagents under suitable conditions respectively for no more than 3 months. Do not use past expiration date.

Background

Introduction
Immune checkpoint is a kind of signal for regulating the antigen recognition of T cell receptor (TCR) in the process of immune responce. Immune checkpoint contains two kinds of signals: (1) Co-stimulatory immune checkpoint: stimulating immune progress, such as CD28, ICOS, and CD137; (2) Co-inhibitory immune checkpoint: inhibiting immune progress, such as PD1, CTLA-4, and VISTA. When immune system is attacking pathogens, these immune checkpoint molecules can protect the normal tissues from damage. The cancer cells cleverly escape from immune attack by dysregulating immune checkpoint related proteins. Immune checkpoint therapy relys on functioning immune system with agonists of co-stimulatory signals or antagonists of inhibitory signals.
Ask a question We look forward to hearing from you.
0 reviews or Q&As

Purchasing FAQs
Technical FAQs

Loading...
Have you used Rat Immune Checkpoint Antibody Array (Membrane, 9 Targets) (Y0542)?
Submit a review and get a Coupon or an Amazon gift card. 20% off Coupon $30 eGift Card
Submit a review
Loading...
For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

Online Inquiry

Documents

Contact us

  • Tel: (USA)
  • (UK)
  • Fax:
  • Email:

Submit A Review

Go to
Compare