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Human Immune Checkpoint Antibody Array (Glass Slide, 10 Targets) (Y0543) (AbAr-0543-YC)

Antibody array is a specific form of protein microarray. In this technology, capture antibodies spotted on membranes or glass slide bind to specific target proteins present in the sample. Captured proteins are visualized using chemiluminescent or Laser Scanner. The signal produced is proportional to the amount of analyte bound. The product provided herein is a Human Immune Checkpoint Antibody Array, which can be used for semi-quantitative detection of 10 human immune checkpoint molecules. It is suitable for a range of liquid forms including Cell culture supernatants, Cell lysates, Plasma, Serum, Tissue lysates and Other body fluids.
Specifications
Target Array
Immune Checkpoint Array
Target
B7-H2(ICOS Ligand), B7-H3(CD276), CD28, CD80(B7-1), CD86(B7-2), CTLA-4(CD152), ICOS, PD-1, PD-L1(B7-H1/CD274), PD-L2(B7-DC)
Species Reactivity
Human
Sample Type
Cell culture supernatants, Cell lysates, Plasma, Serum, Tissue lysates and Other body fluids
Applications
Application
Multiplexed Protein Detection
Assay Type
Semi-Quantitative
Solid Support
Glass Slide
Detection Assay
Sandwich-based Assay
Protocol Outline
Dry the array glass slide
Block glass slide surface
Incubate with samples
Incubate with Biotinylated Detection Antibody Cocktail
Incubate with Fluor Conjugated Streptavidin
Scan with an antibody array laser scanner
Analyze data
Components
Antibody Array Slide
Labeling Reagent
Blocking Buffer
Washing Buffer
Detection Buffer
Detection Cocktail
Others
Storage
Store at -20°C for 6 months. Once thawed, please keep reagents under suitable conditions respectively for no more than 3 months. Do not use past expiration date.
Background
Introduction
Immune checkpoint is a kind of signal for regulating the antigen recognition of T cell receptor (TCR) in the process of immune responce. Immune checkpoint contains two kinds of signals: (1) Co-stimulatory immune checkpoint: stimulating immune progress, such as CD28, ICOS, and CD137; (2) Co-inhibitory immune checkpoint: inhibiting immune progress, such as PD1, CTLA-4, and VISTA. When immune system is attacking pathogens, these immune checkpoint molecules can protect the normal tissues from damage. The cancer cells cleverly escape from immune attack by dysregulating immune checkpoint related proteins. Immune checkpoint therapy relys on functioning immune system with agonists of co-stimulatory signals or antagonists of inhibitory signals.
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For Research Use Only. Not For Clinical Use.