Mouse Recombinant EphB3 protein, His Tag (V2LY-0526-LY8254)

Name: Mouse Recombinant EphB3 protein, His Tag
SKU: V2LY-0526-LY8254
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

HEK293 Cells

Protein Species

Mouse

Tag

His Tag

Protein Construction

This product is Mouse Recombinant EphB3 protein, His Tag consist of Amino Acid: 1-537 and predicts a molecular mass of 57 kDa.

Molecule Mass

57 kDa

Verified

HPLC

Sequence

Amino Acid: 1-537

Species

Mouse

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

≥94% as determined by SDS-PAGE. ≥90% as determined by SEC-HPLC.

Endotoxin

Please contact us for more information.

Format

Lyophilized

Reconstitution

Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.

Buffer

Lyophilized from sterile PBS

Preservative

None

Storage

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

More Infomation

Target

Full Name

EPH Receptor B3

Research Area

Receptor tyrosine kinase which binds promiscuously transmembrane ephrin-B family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Generally has an overlapping and redundant function with EPHB2. Like EPHB2, functions in axon guidance during development regulating for instance the neurons forming the corpus callosum and the anterior commissure, 2 major interhemispheric connections between the temporal lobes of the cerebral cortex. In addition to its role in axon guidance plays also an important redundant role with other ephrin-B receptors in development and maturation of dendritic spines and the formation of excitatory synapses. Controls other aspects of development through regulation of cell migration and positioning. This includes angiogenesis, palate development and thymic epithelium development for instance. Forward and reverse signaling through the EFNB2/EPHB3 complex also regulate migration and adhesion of cells that tubularize the urethra and septate the cloaca. Finally, plays an important role in intestinal epithelium differentiation segregating progenitor from differentiated cells in the crypt.

Biological Process

Angiogenesis Source: UniProtKB
Axonal fasciculation Source: UniProtKB
Axon guidance Source: UniProtKB
Cell migration Source: UniProtKB
Central nervous system projection neuron axonogenesis Source: Ensembl
Corpus callosum development Source: UniProtKB
Dendritic spine development Source: UniProtKB
Dendritic spine morphogenesis Source: UniProtKB
Digestive tract morphogenesis Source: UniProtKB
Ephrin receptor signaling pathway Source: UniProtKB
Positive regulation of kinase activity Source: GO_Central
Positive regulation of synapse assembly Source: UniProtKB
Protein autophosphorylation Source: UniProtKB
Regulation of axonogenesis Source: UniProtKB
Regulation of cell-cell adhesion Source: UniProtKB
Regulation of GTPase activity Source: UniProtKB
Retinal ganglion cell axon guidance Source: Ensembl
Roof of mouth development Source: UniProtKB
Substrate adhesion-dependent cell spreading Source: UniProtKB
Thymus development Source: UniProtKB
Transmembrane receptor protein tyrosine kinase signaling pathway Source: GO_Central
Urogenital system development Source: UniProtKB

Cellular Location

Cell membrane; Dendrite

Topology

Extracellular: 34-559
Helical: 560-580
Cytoplasmic: 581-998

PTM

Phosphorylated. Autophosphorylates upon ligand-binding. Autophosphorylation on Tyr-614 is required for interaction with SH2 domain-containing proteins.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Human Recombinant EphB3, Active protein, GST Tag (CAT#: V2LY-0526-LY3902)

Rat Recombinant EphB3 protein, ECD, His Tag (CAT#: V2LY-0526-LY9357)

Human EPHB3 ELISA Kit (CAT#: V2LY-0626-LY4551)

EPHB3 Matched Antibody Pair (385) (CAT#: APMAB-385LY)

Antibody Pairs

EPHB3 Matched Antibody Pair (385) (CAT#: APMAB-385LY)

Related Products

Human EPHB3 ELISA Kit (CAT#: V2LY-0626-LY4551)

Mouse Anti-EPHB3 Recombinant Antibody (CBFYE-1015) (CAT#: CBMAB-E1517-FY)

Human Recombinant EphB3, Active protein, GST Tag (CAT#: V2LY-0526-LY3902)

Rat Anti-EPHB3 Recombinant Antibody (CBFYE-1014) (CAT#: CBMAB-E1515-FY)

Mouse Anti-EPHB3 Recombinant Antibody (64) (CAT#: CBMAB-E0225-FY)

Rabbit Anti-EPHB3 Recombinant Antibody (CBFYE-0055) (CAT#: CBMAB-E0135-FY)

Mouse Anti-EPHB3 Recombinant Antibody (41) (CAT#: CBMAB-AO402LY)

Mouse Anti-EPHB3 Recombinant Antibody (41) (CAT#: CBMAB-E1522-FY)

Rabbit Anti-EPHB3 Recombinant Antibody (CBFYE-1017) (CAT#: CBMAB-E1519-FY)

Mouse Anti-EPHB3 Recombinant Antibody (68) (CAT#: CBMAB-E1516-FY)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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