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Human RTK Phosphorylation Antibody Array (Glass Slide, 71 Targets) (Y0054) (AbAr-0054-YC)

Antibody array is a specific form of protein microarray. In this technology, capture antibodies spotted on membranes or glass slide bind to specific target proteins present in the sample. Captured proteins are visualized using chemiluminescent or Laser Scanner. The signal produced is proportional to the amount of analyte bound. The Human RTK Phosphorylation Antibody Array detects 71 Human RTKs. It is suitable for all liquid sample types but intended for use with cell and tissue lysates.
Target Array
RTK Phosphorylation Array
ABL1, BMX, EphA1, EphA6, EphB3, ErbB4, FGFR2 alpha isoform, HGFR, JAK2, MATK, PDGFRB, ROS, Tec, TXK, ZAP70, ACK, Btk, EphA2, EphA7, EphB4, FAK, Fgr, IGF-1 R, JAK3, M-CSF R, PYK2, RYK, Tie-1, Tyk2, ALK-1, Csk, EphA3, EphA8, EphB6, FER, FRK, Insulin R (CD220), LCK, MUSK, RET, SCF R (CD117/c-kit), Tie-2, TYRO10 (DDR2/TKT), Axl, Dtk, EphA4, EphB1, ErbB2, FGFR1, Fyn, Itk, LTK, NGFR (TNFRSF16), ROR1, SRMS, TNK1, VEGFR2, Blk, EGFR, EphA5, EphB2, ErbB3, FGFR2, Hck, JAK1, Lyn, PDGFRA, ROR2, SYK, TRKB, VEGFR3
Species Reactivity
Sample Type
Serum, Plasma, Cell Culture Supernatant, Cell Lysates, Tissue Lysates
Multiplex Protein Detection
Assay Type
Solid Support
Glass Slide
Detection Assay
Protocol Outline
Dry the glass slide
Block array surface
Incubate with Sample
Incubate with Biotinylated Detection Antibody Cocktail
Incubate with Streptavidin-Conjugated Fluor
Disassemble the glass slide
Scan with a gene microarray laser scanner
Perform densitometry and analysis
Antibody Array Slide
Labeling Reagent
Blocking Buffer
Wash Buffer
Detection Buffer
Detection Cocktail
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Once thawed, please keep reagents under suitable conditions respectively. Do not use past expiration date.
Receptor tyrosine kinases (RTKs) are the high-affinity cell surface receptors for many polypeptide growth factors, cytokines, and hormones. Of the 90 unique tyrosine kinase genes identified in the human genome, 58 encode receptor tyrosine kinase proteins. Receptor tyrosine kinases have been shown not only to be key regulators of normal cellular processes but also to have a critical role in the development and progression of many types of cancer. Mutations in receptor tyrosine kinases lead to activation of a series of signalling cascades which have numerous effects on protein expression. Receptor tyrosine kinases are part of the larger family of protein tyrosine kinases, encompassing the receptor tyrosine kinase proteins which contain a transmembrane domain, as well as the non receptor tyrosine kinases which do not possess transmembrane domains.
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For Research Use Only. Not For Clinical Use.