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Human RTK Phosphorylation Antibody Array (Membrane, 71 Targets) (Y0045) (AbAr-0045-YC)

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Specifications

Target Array
RTK Phosphorylation Array
Target
ABL1, BMX, EphA1, EphA6, EphB3, ErbB4, FGFR2 alpha isoform, HGFR, JAK2, MATK, PDGFRB, ROS, Tec, TXK, ZAP70, ACK, Btk, EphA2, EphA7, EphB4, FAK, Fgr, IGF-1 R, JAK3, M-CSF R, PYK2, RYK, Tie-1, Tyk2, ALK-1, Csk, EphA3, EphA8, EphB6, FER, FRK, Insulin R, LCK, MUSK, RET, SCF R, Tie-2, TYRO10, Axl, Dtk, EphA4, EphB1, ErbB2, FGFR1, Fyn, Itk, LTK, NGFR, ROR1, SRMS, TNK1, VEGFR2, Blk, EGFR, EphA5, EphB2, ErbB3, FGFR2, Hck, JAK1, Lyn, PDGFRA, ROR2, SYK, TRKB, VEGFR3
Species Reactivity
Human
Sample Type
Serum, Plasma, Cell Culture Supernatant, Cell Lysates, Tissue Lysates

Applications

Application
Multiplex Protein Detection
Assay Type
Semi-Quantitative
Solid Support
Membrane
Detection Assay
Sandwich-based
Protocol Outline
Apply a blocking agent to the membranes.
Proceed with sample incubation.
Incubate using the cocktail of biotinylated detection antibodies.
Incubate with streptavidin conjugated to horseradish peroxidase (HRP).
Conduct incubation with the detection buffers.
Utilize a chemiluminescent imaging system for image capture.
Execute densitometric evaluation and perform data analysis.
Components
Antibody Array Membrane
Labeling Reagent
Blocking Buffer
Wash Buffer
Detection Buffer
Detection Cocktail
Others
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Once thawed, please keep reagents under suitable conditions respectively. Do not use past expiration date.

Background

Introduction
Receptor tyrosine kinases (RTKs) are the high-affinity cell surface receptors for many polypeptide growth factors, cytokines, and hormones. Of the 90 unique tyrosine kinase genes identified in the human genome, 58 encode receptor tyrosine kinase proteins. Receptor tyrosine kinases have been shown not only to be key regulators of normal cellular processes but also to have a critical role in the development and progression of many types of cancer. Mutations in receptor tyrosine kinases lead to activation of a series of signalling cascades which have numerous effects on protein expression. Receptor tyrosine kinases are part of the larger family of protein tyrosine kinases, encompassing the receptor tyrosine kinase proteins which contain a transmembrane domain, as well as the non receptor tyrosine kinases which do not possess transmembrane domains.
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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

Alternative Versions

Human RTK Phosphorylation Antibody Array (Membrane, 49 Targets) (Y0228) (CAT#: AbAr-0228-YC)

Mouse RTK Phosphorylation Antibody Array (Membrane, 39 Targets) (Y0237) (CAT#: AbAr-0237-YC)

Human RTK Phosphorylation Antibody Array (Glass Slide, 71 Targets) (Y0054) (CAT#: AbAr-0054-YC)

Antibody Array

Human RTK Phosphorylation Antibody Array (Membrane, 49 Targets) (Y0228) (CAT#: AbAr-0228-YC)

Mouse RTK Phosphorylation Antibody Array (Membrane, 39 Targets) (Y0237) (CAT#: AbAr-0237-YC)

Human RTK Phosphorylation Antibody Array (Glass Slide, 71 Targets) (Y0054) (CAT#: AbAr-0054-YC)

Related Products

Human RTK Phosphorylation Antibody Array (Membrane, 49 Targets) (Y0228) (CAT#: AbAr-0228-YC)

Mouse RTK Phosphorylation Antibody Array (Membrane, 39 Targets) (Y0237) (CAT#: AbAr-0237-YC)

Human RTK Phosphorylation Antibody Array (Glass Slide, 71 Targets) (Y0054) (CAT#: AbAr-0054-YC)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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