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Human RTK Phosphorylation Antibody Array (Membrane, 49 Targets) (Y0228) (AbAr-0228-YC)

Antibody array is a specific form of protein microarray. In this technology, capture antibodies spotted on membranes or glass slide bind to specific target proteins present in the sample. Captured proteins are visualized using chemiluminescent or Laser Scanner. The signal produced is proportional to the amount of analyte bound. The Human Phospho-RTK Antibody Array detects phosphorylation of 49 human receptor tyrosine kinases simultaneously.
Specifications
Target Array
RTK Phosphorylation Array
Target
ALK/CD246, EphB4, MuSK, Axl, EphB6, PDGF R alpha, DDR1, ErbB2, PDGF R beta, DDR2, ErbB3, c-Ret, Dtk, ErbB4, ROR1, EGF R, FGF R1, ROR2, EphA1, FGF R2 alpha, Ryk, EphA2, FGF R3, SCF R/c-kit, EphA3, FGF R4, Tie-1, EphA4, Flt-3/Flk-2, Tie-2, EphA5, HGF R/c-MET, TrkA, EphA6, IGF-I R, TrkB, EphA7, Insulin R/CD220, TrkC, EphA10, M-CSF R, VEGF R1/Flt-1, EphB1, Mer, VEGF R2/KDR, EphB2, MSP R/Ron, VEGF R3/Flt-4, EphB3
Species Reactivity
Human
Sample Type
Cell Lysates
Applications
Application
Multiplex Protein Detection
Solid Support
Membrane
Detection Assay
Sandwich-based
Protocol Outline
Block membranes
Incubate with Sample
Incubate with Biotinylated Detection Antibody Cocktail
Incubate with HRP-Conjugated Streptavidin
Incubate with Detection Buffers
Image with chemiluminescent imaging system
Perform densitometry and analysis
Components
Antibody Array Membrane
Labeling Reagent
Blocking Buffer
Wash Buffer
Detection Buffer
Detection Cocktail
Others
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Once thawed, please keep reagents under suitable conditions respectively. Do not use past expiration date.
Background
Introduction
Receptor tyrosine kinases (RTKs) are the high-affinity cell surface receptors for many polypeptide growth factors, cytokines, and hormones. Of the 90 unique tyrosine kinase genes identified in the human genome, 58 encode receptor tyrosine kinase proteins. Receptor tyrosine kinases have been shown not only to be key regulators of normal cellular processes but also to have a critical role in the development and progression of many types of cancer. Mutations in receptor tyrosine kinases lead to activation of a series of signalling cascades which have numerous effects on protein expression. Receptor tyrosine kinases are part of the larger family of protein tyrosine kinases, encompassing the receptor tyrosine kinase proteins which contain a transmembrane domain, as well as the non receptor tyrosine kinases which do not possess transmembrane domains.
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For research use only. Not intended for any clinical use.