ADCY5 Antibodies

Structure of ADCY5

The adenylate cyclase 5 encoded by the ADCY5 gene is a transmembrane protein with a molecular weight of approximately 135 kDa. The amino acid sequence of this enzyme is highly conserved among different species. This enzyme consists of two transmembrane domains and two catalytic domains, and it participates in intracellular signal transduction by catalyzing the generation of cAMP from ATP.

The ADCY5 protein is composed of 1264 amino acids and its secondary structure is rich in α-helices, forming a characteristic transmembrane topology. The two catalytic domains C1 and C2 form a dimer within the cell, constituting the ATP binding site. This enzyme is regulated by both the α subunit and the βγ subunits of the G protein, and its active region contains multiple phosphorylation sites that can be phosphorylated by PKA and PKC, thereby achieving precise regulation of cAMP production.

Fig. 1 Urine Derivatives Inhibit ADCY5 Activity by Blocking the A2A Receptor.¹

The core structural features of ADCY5:

• 12 transmembrane structures
• The C1 and C2 catalytic domains form a dimer
• G protein binding site
• PKA/PKC phosphorylation sites

Functions of ADCY5

ADCY5, as a member of the adenylate cyclase family, primarily functions by catalyzing the conversion of ATP into the second messenger cAMP. However, it is involved in the regulation of various physiological processes in different tissues.

The basal activity of ADCY5 is directly regulated by the α subunit of G protein. It has a relatively low sensitivity to calcium ions and mainly relies on the activation mode mediated by G protein-coupled receptors.

Applications of ADCY5 and ADCY5 Antibody in Literature

1. Tänzler, Dirk, et al. "Effects of theophylline on ADCY5 activation—From cellular studies to improved therapeutic options for ADCY5-related dyskinesia patients." PLoS One 18.3 (2023): e0282593. https://doi.org/10.1371/journal.pone.0282593

The article indicates that all three purine derivatives can reduce the generation of cAMP catalyzed by ADCY5, and their effects are most significant on the diseased R418W cells. Based on this finding, a preschooler was treated with a sustained-release formulation of theophylline, and the symptoms improved significantly and were more effective than caffeine. This suggests that theophylline can be a potential treatment option for ADCY5-related movement disorders.

2. Can, Wang, et al. "ADCY5 act as a putative tumor suppressor in glioblastoma: an integrated analysis." Heliyon 10.17 (2024). https://doi.org/10.1016/j.heliyon.2024.e37012

The study found that ADCY5 is downregulated in glioblastoma due to DNA methylation, and its high expression is associated with a better prognosis for patients. In vitro experiments confirmed that restoring ADCY5 expression can inhibit tumor cell proliferation, migration, and epithelial-mesenchymal transition, suggesting that it can serve as an epigenetic marker and prognostic indicator for glioblastoma.

3. Chen, Shih-Ying, et al. "ADCY5-related dyskinesia: a case report." Neurological Research and Practice 4.1 (2022): 39. https://doi.org/10.1186/s42466-022-00204-w

The study found that ADCY5-related movement disorders are a rare disease characterized by early-onset paroxysmal choreoathetosis of the hands and feet, dystonia, etc., which often affects the limbs, face and neck, and may be accompanied by axial hypotonia. This article reports the first confirmed case in Taiwan.

4. Innocenti, Alice, et al. "ADCY5‐mosaic variants: a diagnosis not to be missed." Movement Disorders Clinical Practice 12.11 (2025): 1968-1971. https://doi.org/10.1002/mdc3.70175

The study found that the phenotypes of patients with ADCY5 chimeric mutations were similar to those of non-chimeric patients but were milder. 63% presented with basal motor disorders accompanied by paroxysmal exacerbation, and 30% had isolated paroxysmal motor disorders. Caffeine treatment was effective. The isolated paroxysmal motor disorder that onset in infancy is a warning sign of this disease and is prone to be missed.

5. Zhang, Lin, et al. "Requirement of zebrafish Adcy3a and Adcy5 in melanosome dispersion and melanocyte stripe formation." International Journal of Molecular Sciences 23.22 (2022): 14182. https://doi.org/10.3390/ijms232214182

The study found that adenylate cyclase Adcy3a and Adcy5 have redundant functions in zebrafish, regulating melanosomes dispersion and adult melanin synthesis through the PKA signaling pathway. The absence of either of them does not affect the development of melanocytes in embryos, but causes melanosomes aggregation, defects in adult stripes formation, and reduction of pigmentation.

Creative Biolabs: ADCY5 Antibodies for Research

Creative Biolabs specializes in the production of high-quality ADCY5 antibodies for research and industrial applications. Our portfolio includes monoclonal and polyclonal antibodies tailored for ELISA, Flow Cytometry, Western blot, immunohistochemistry, and other diagnostic methodologies.

• Custom ADCY5 Antibody Development: Tailor-made solutions to meet specific research requirements.
• Bulk Production: Large-scale antibody manufacturing for industry partners.
• Technical Support: Expert consultation for protocol optimization and troubleshooting.
• Aliquoting Services: Conveniently sized aliquots for long-term storage and consistent experimental outcomes.

For more details on our ADCY5 antibodies, custom preparations, or technical support, contact us at email.