CA8 Antibodies

Structure of CA8

The CA8 gene encodes the carbonic anhydrase-related protein 8 (CA8), which is a protein with a molecular weight of approximately 28-30 kDa. The exact molecular weight varies slightly among different species and is mainly influenced by post-translational modifications.

The CA8 protein is composed of approximately 260 amino acids and exhibits a typical folding structure of the carbonic anhydrase β family. However, the key histidine residues at the active site have been replaced, thus losing the classic catalytic function of carbonic anhydrase. Its tertiary structure forms a compact spherical core, mainly composed of β-sheet structures and containing several key surface loop regions. These loop regions are responsible for interacting with target proteins (such as the receptor-type tyrosine phosphatase RPTPβ), thereby playing an important regulatory role in neural development and cerebellar motor coordination.

Fig. 1 Three-Dimensional Structural Features of Human CA-VIII.¹

Key structural properties of CA8:

• With typical of carbonic anhydrase beta family beta folding core barrel structure
• Active site key histidine replaced, which leads to the absence of catalytic function
• The surface loop region determines its specific protein-protein interaction ability
• By zinc ions structure variation to realize the control function of binding sites

Functions of CA8

The main function of the protein encoded by the CA8 gene is to participate in neuronal signal transmission and maintain calcium homeostasis in the cerebellum. Although it lacks carbonic anhydrase activity, it plays a crucial role in various physiological processes.

The mechanism of action of CA8 protein does not rely on typical enzymatic reactions. Instead, it achieves its purpose through its unique protein interaction interface, binding with specific target proteins with high affinity, thereby precisely regulating the downstream signaling network. This is in sharp contrast to the catalytically active members of the classical carbonic anhydrase family.

Applications of CA8 and CA8 Antibody in Literature

1. Sanyanga, Taremekedzwa Allan, and Özlem Tastan Bishop. "Structural characterization of carbonic anhydrase VIII and effects of missense single nucleotide variations to protein structure and function." International Journal of Molecular Sciences 21.8 (2020): 2764. https://doi.org/10.3390/ijms21082764

The article indicates that although carbonic anhydrase VIII (CA8) has no catalytic activity, it is crucial for maintaining calcium homeostasis. In this study, through molecular simulation analysis of pathogenic mutations, it was found that these mutations increase protein rigidity and affect the accessibility of binding sites, providing a new direction for elucidating the mechanisms of related diseases.

2. Yang, Meiyan, et al. "Isolation and characterization of the novel phages vB_VpS_BA3 and vB_VpS_CA8 for lysing Vibrio parahaemolyticus." Frontiers in microbiology 11 (2020): 259. https://doi.org/10.3389/fmicb.2020.00259

The article indicates that a new type of bacteriophage vB_VpS_CA8 has been isolated, which has a lytic effect on multi-drug resistant Vibrio parahaemolyticus. In vitro, it can reduce the bacterial count by approximately 3.65 logarithmic units, and it is expected to be applied in biological control.

3. Türkmen, Seval, et al. "CA8 mutations cause a novel syndrome characterized by ataxia and mild mental retardation with predisposition to quadrupedal gait." PLoS genetics 5.5 (2009): e1000487. https://doi.org/10.1371/journal.pgen.1000487

In an Iraqi consanguineous family, the patient suffered from a homozygous mutation of S100P in the CA8 gene, which led to the degradation of this protein and disrupted the calcium signal transduction in the cerebellum. As a result, the patient exhibited intellectual disability, ataxia, and a rare quadrupedal gait.

4. Peng, Jianya, et al. "Carbonic anhydrase 8 regulates basophil activation and interleukin 4 production." Frontiers in immunology 16 (2025): 1694105. https://doi.org/10.3389/fimmu.2025.1694105

The article indicates that carbonic anhydrase 8 is highly expressed in basophilic granulocytes. The study found that although Car8-deficient mice do not affect the development of basophilic granulocytes, they can inhibit the release of IL-4 and improve dermatitis-like inflammation, revealing its new function in the regulation of basophilic granulocyte activation.

5. Ma, Huai-Lu, et al. "CA8 promotes RCC proliferation and migration though its expression level is lower in tumor compared to adjacent normal tissue." Biomedicine & Pharmacotherapy 121 (2020): 109578. https://doi.org/10.1016/j.biopha.2019.109578

The article indicates that in renal cell carcinoma, the CA8 gene shows low expression. However, experiments have confirmed that its overexpression can promote cancer cell proliferation and migration by activating the pAKT/MMP2 pathway.

Creative Biolabs: CA8 Antibodies for Research

Creative Biolabs specializes in the production of high-quality CA8 antibodies for research and industrial applications. Our portfolio includes monoclonal and polyclonal antibodies tailored for ELISA, Flow Cytometry, Western blot, immunohistochemistry, and other diagnostic methodologies.

• Custom CA8 Antibody Development: Tailor-made solutions to meet specific research requirements.
• Bulk Production: Large-scale antibody manufacturing for industry partners.
• Technical Support: Expert consultation for protocol optimization and troubleshooting.
• Aliquoting Services: Conveniently sized aliquots for long-term storage and consistent experimental outcomes.

For more details on our CA8 antibodies, custom preparations, or technical support, contact us at email.