Human Recombinant CA8 protein, His Tag (V2LY-0526-LY2548)

Name: Human Recombinant CA8 protein, His Tag
SKU: V2LY-0526-LY2548
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

E. coli

Protein Species

Human

Tag

His Tag

Protein Construction

This product is Human Recombinant CA8 protein, His Tag consist of Amino Acid: 1-290 and predicts a molecular mass of 33.8 kDa.

Molecule Mass

33.8 kDa

Sequence

Amino Acid: 1-290

Species

Human

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

≥90% as determined by SDS-PAGE

Endotoxin

Please contact us for more information.

Format

Lyophilized

Reconstitution

Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.

Buffer

Lyophilized from sterile PBS

Preservative

None

Storage

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

More Infomation

Target

Full Name

CA8

Function

Does not have a carbonic anhydrase catalytic activity.

Biological Process

One-carbon metabolic process Source: GO_Central
Phosphatidylinositol-mediated signaling Source: Ensembl

Cellular Location

Cytoplasm

Involvement in disease

Cerebellar ataxia, mental retardation, and dysequilibrium syndrome 3 (CMARQ3): A congenital cerebellar ataxia associated with dysarthia, quadrupedal gait and mental retardation.

Chi, T. H., Hsieh, B. Y., Liang, P. S., Han, T. H., & Hsieh, M. (2021). Expression and Functional Study of Single Mutations of Carbonic Anhydrase 8 in Neuronal Cells. Cellular and Molecular Neurobiology, 41(6), 1355-1371.

Mamoor, S. (2021). Carbonic anhydrase VIII (CA8) is a differentially expressed gene in brain metastatic human breast cancer.

Paternoster, L., Soblet, J., Aeby, A., De Tiège, X., Goldman, S., Yue, W. W., ... & Deconinck, N. (2020). Novel homozygous variant of carbonic anhydrase 8 gene expanding the phenotype of cerebellar ataxia, mental retardation, and disequilibrium syndrome subtype 3. American Journal of Medical Genetics Part A, 182(11), 2685-2693.

Sanyanga, T. A., & Tastan Bishop, Ö. (2020). Structural characterization of carbonic anhydrase VIII and effects of missense single nucleotide variations to protein structure and function. International journal of molecular sciences, 21(8), 2764.

Hsieh, M., Hsieh, B. Y., Ma, C. Y., Li, Y. T., Liu, C. S., & Lo, C. M. (2019). Protective roles of carbonic anhydrase 8 in Machado–Joseph Disease. Journal of neuroscience research, 97(10), 1278-1297.

Lo, C. M., Ma, Y. S., Wei, Y. H., Hsieh, B. Y., & Hsieh, M. (2018). Promoter analysis and transcriptional regulation of human carbonic anhydrase VIII gene in a MERRF disease cell model. Archives of biochemistry and biophysics, 641, 50-61.

Zhuang, G. Z., Upadhyay, U., Tong, X., Kang, Y., Erasso, D. M., Fu, E. S., ... & Levitt, R. C. (2018). Human carbonic anhydrase-8 AAV8 gene therapy inhibits nerve growth factor signaling producing prolonged analgesia and anti-hyperalgesia in mice. Gene therapy, 25(4), 297-311.

Paternoster, L., Soblet, J., Aeby, A., Vilain, C., Smits, G., & Deconinck, N. (2017). A new mutation of carbonic anhydrase 8 gene expanding the cerebellar ataxia, mental retardation and disequilibrium syndrome (CAMRQ) subtype 3. J Neurol Sci, 381, 1136-7.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Mouse Recombinant CA8 protein, His Tag (CAT#: V2LY-0526-LY7904)

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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