CX3CL1 Antibodies

Structure of CX3CL1

CX3CL1 is a transmembrane chemokine with a molecular weight of approximately 40-95 kDa, and the specific value varies depending on different spliceosomes and glycosylation modifications. Its structure is mainly composed of chemokine domains, mucin-like domains, transmembrane domains and intracellular tails. This protein stabilizes its tertiary structure through disulfide bonds and glycosylation modifications. The key proximal cysteine residue maintains the folding of core chemokines, while the distal glycosylation site regulates the binding specificity to the receptor CX3CR1. Its unique "style-sphere" conformation can directly mediate the stable adhesion and signal transduction between immune cells and endothelial cells.

Fig. 1 Schematic structure of the C-X3-C motif chemokine ligand 1 (CX3CL1).¹

Key structural properties of CX3CL1:

• Unique "ball-handle type" transmembrane structure
• Highly conserved chemokine domains (CXC motifs)
• Mucin-like stem regions rich in serine/threonine
• Metalloprotease cleavage sites in the juxtamembrane region

Functions of CX3CL1

The core function of CX3CL1 (fractal chemokine) is to mediate the chemotaxis and adhesion of immune cells. In addition, it is also involved in a variety of physiological and pathological processes, including neuroprotection, angiogenesis and inflammatory regulation.

The binding of CX3CL1 to its receptor CX3CR1 exhibits typical chemokine cascade reaction characteristics. Unlike the transient signals of most chemokines, its membrane-bound form can provide persistent adhesion signals, which enables it to play a unique role in immune surveillance and tissue homeostasis maintenance.

Applications of CX3CL1 and CX3CL1 Antibody in Literature

1. Gutiérrez, Irene L., et al. "CX3CL1 Regulation of Gliosis in Neuroinflammatory and Neuroprotective Processes." International Journal of Molecular Sciences 26.3 (2025): 959. https://doi.org/10.3390/ijms26030959

The article indicates that CX3CL1 is a structurally unique chemokine that participates in the communication between neurons and microglia in a membrane-bound and soluble form, regulating neuroinflammation. Its role in neurodegenerative diseases such as Alzheimer's disease and Parkinson's disease has attracted much attention, but differences in research models have led to inconsistent results.

2. Korbecki, Jan, et al. "Fractalkine/CX3CL1 in neoplastic processes." International journal of molecular sciences 21.10 (2020): 3723. https://doi.org/10.3390/ijms21103723

The article indicates that CX3CL1 plays a complex role in cancer. It can enhance the anti-cancer function of immune cells and improve prognosis, as well as promote angiogenesis, metastasis and other malignant processes. Through interactions with various cells in the tumor microenvironment, it affects neuroinvasion, bone metastasis, etc., and may become a target for immunotherapy.

3. Wątroba, Mateusz, Anna D. Grabowska, and Dariusz Szukiewicz. "Chemokine CX3CL1 (Fractalkine) signaling and diabetic encephalopathy." International Journal of Molecular Sciences 25.14 (2024): 7527. https://doi.org/10.3390/ijms25147527

The article indicates that CX3CL1 regulates microglial cell activity through its receptor CX3CR1. In diabetic encephalopathy, it plays a dual role, being able to provide neuroprotection as well as potentially exacerbating neuroinflammation and toxicity. It is a potential therapeutic target, but controlling blood sugar remains the fundamental preventive measure.

4. Szukiewicz, Dariusz. "CX3CL1 (Fractalkine)-CX3CR1 Axis in inflammation-induced angiogenesis and tumorigenesis." International journal of molecular sciences 25.9 (2024): 4679. https://doi.org/10.3390/ijms25094679

The article indicates that the chemokine CX3CL1 possesses both chemotactic and adhesion functions. It regulates inflammation and immune responses through its receptor CX3CR1, and influences angiogenesis and cancer metastasis in hypoxic and tumor microenvironments. The signaling pathway of this molecule is complex, exerting both oncogenic and tumor-suppressive effects, resulting in seemingly contradictory research findings.

5. Zhang, Chunmei, et al. "Alterations in CX3CL1 levels and its role in viral pathogenesis." International Journal of Molecular Sciences 25.8 (2024): 4451. https://doi.org/10.3390/ijms25084451

The article indicates that CX3CL1 (fractalkine), as a unique chemokine, regulates the chemotaxis of immune cells and the activation of central nervous system microglia through the receptor CX3CR1. Studies have shown that it participates in regulating cell adhesion and host immune responses during various viral infections such as HIV and COVID-19, and its role is receiving increasing attention.

Creative Biolabs: CX3CL1 Antibodies for Research

Creative Biolabs specializes in the production of high-quality CX3CL1 antibodies for research and industrial applications. Our portfolio includes monoclonal antibodies tailored for ELISA, Flow Cytometry, Western blot, immunohistochemistry, and other diagnostic methodologies.

• Custom CX3CL1 Antibody Development: Tailor-made solutions to meet specific research requirements.
• Bulk Production: Large-scale antibody manufacturing for industry partners.
• Technical Support: Expert consultation for protocol optimization and troubleshooting.
• Aliquoting Services: Conveniently sized aliquots for long-term storage and consistent experimental outcomes.

For more details on our CX3CL1 antibodies, custom preparations, or technical support, contact us at email.