Mouse Anti-CDK5RAP2 Recombinant Antibody (CBYY-C1777) (CBMAB-C3215-YY)

This product is mouse antibody that recognizes CDK5RAP2. The antibody CBYY-C1777 can be used for immunoassay techniques such as: WB, ELISA
See all CDK5RAP2 antibodies

Datasheet

Summary

Host Animal

Mouse

Specificity

Human

Clone

CBYY-C1777

Antibody Isotype

IgM

Application

WB, ELISA

Basic Information

Immunogen

This CDK5RAP2 Antibody is generated from mouse immunized with recombinant protein of human CDK5RAP2

Specificity

Human

Antibody Isotype

IgM

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Concentration

Liquid

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name

CDK5 Regulatory Subunit Associated Protein 2

Entrez Gene ID

55755

UniProt ID

Q96SN8

Alternative Names

Cyclin Dependent Kinase Inhibitor 2A; Cyclin-Dependent Kinase Inhibitor 2A (Melanoma, P16, Inhibits CDK4); Cyclin-Dependent Kinase 4 Inhibitor A; Cyclin-Dependent Kinase Inhibitor 2A; Multiple Tumor Suppressor 1; Alternative Reading Frame; P16-INK4A; P16INK4A; P14ARF; CDKN2; CDK4I; MTS-1; MTS1; MLM;

Function

Potential regulator of CDK5 activity via its interaction with CDK5R1. Negative regulator of centriole disengagement (licensing) which maintains centriole engagement and cohesion. Involved in regulation of mitotic spindle orientation (By similarity).
Plays a role in the spindle checkpoint activation by acting as a transcriptional regulator of both BUBR1 and MAD2 promoter. Together with EB1/MAPRE1, may promote microtubule polymerization, bundle formation, growth and dynamics at the plus ends. Regulates centrosomal maturation by recruitment of the gamma-tubulin ring complex (gamma-TuRC) onto centrosomes (PubMed:26485573).
In complex with PDE4DIP isoform 13/MMG8/SMYLE, MAPRE1 and AKAP9, contributes to microtubules nucleation and extension from the centrosome to the cell periphery (PubMed:29162697).
Required for the recruitment of AKAP9 to centrosomes (PubMed:29162697).
Plays a role in neurogenesis (By similarity).

Biological Process

Brain development Source: UniProtKB
Centriole replication Source: UniProtKB
Centrosome cycle Source: UniProtKB
Chromosome segregation Source: UniProtKB
Ciliary basal body-plasma membrane docking Source: Reactome
Establishment of mitotic spindle orientation Source: UniProtKB
G2/M transition of mitotic cell cycle Source: Reactome
Microtubule bundle formation Source: UniProtKB
Microtubule cytoskeleton organization Source: UniProtKB
Microtubule organizing center organization Source: UniProtKB
Negative regulation of centriole replication Source: UniProtKB
Negative regulation of neuron differentiation Source: Ensembl
Neurogenesis Source: UniProtKB
Positive regulation of microtubule polymerization Source: UniProtKB
Positive regulation of transcription, DNA-templated Source: UniProtKB
Regulation of G2/M transition of mitotic cell cycle Source: Reactome
Regulation of mitotic cell cycle spindle assembly checkpoint Source: UniProtKB
Regulation of neuron differentiation Source: UniProtKB

Cellular Location

Cytoplasm; Golgi apparatus; Centrosome; Cytoskeleton. Found in the pericentriolar region adhering to the surface of the centrosome and in the region of the centrosomal appendages. Localizes to microtubule plus ends in the presence of EB1/MAPRE1. Localization to centrosomes versus Golgi apparatus may be cell type-dependent. For instance, in SK-BR-3 and HEK293F cells, localizes to centrosomes but not to the Golgi apparatus (PubMed:29162697).

Involvement in disease

Microcephaly 3, primary, autosomal recessive (MCPH3): A disease defined as a head circumference more than 3 standard deviations below the age-related mean. Brain weight is markedly reduced and the cerebral cortex is disproportionately small. Despite this marked reduction in size, the gyral pattern is relatively well preserved, with no major abnormality in cortical architecture. Affected individuals are mentally retarded. Primary microcephaly is further defined by the absence of other syndromic features or significant neurological deficits due to degenerative brain disorder.

PTM

Phosphorylated in vitro by CDK5.

References

Wang, X., Rosales, J. L., Gao, X., & Lee, K. Y. (2021). Centromeric chromatin integrity is compromised by loss of Cdk5rap2, a transcriptional activator of CENP-A. Biomedicine & Pharmacotherapy, 138, 111463.

Ayed, I. B., Bouchaala, W., Bouzid, A., Feki, W., Souissi, A., Nsir, S. B., ... & Triki, C. (2021). Further insights into the spectrum phenotype of TRAPPC9 and CDK5RAP2 genes, segregating independently in a large Tunisian family with intellectual disability and microcephaly. European journal of medical genetics, 104373.

Wang, X. (2020). Role of Cdk5rap2 in Cell Senescence and CENP-A-mediated Centromeric Chromatin Integrity.

Alfares, A., Alhufayti, I., Alsubaie, L., Alowain, M., Almass, R., Alfadhel, M., ... & Eyaid, W. (2018). A new association between CDK5RAP2 microcephaly and congenital cataracts. Annals of human genetics, 82(3), 165-170.

Fong, K. W., Au, F. K., Jia, Y., Yang, S., Zhou, L., & Qi, R. Z. (2017). Microtubule plus-end tracking of end-binding protein 1 (EB1) is regulated by CDK5 regulatory subunit-associated protein 2. Journal of Biological Chemistry, 292(18), 7675-7687.

Zaqout, S. (2017). Cyclin-dependent kinase 5 regulatory subunit-associated protein 2 (Cdk5rap2) in neuronal differentiation and germ cell pool maintenance (Doctoral dissertation).

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Protocols

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Associated Products

Related Products

Rabbit Anti-CDK5RAP2 Recombinant Antibody (EG609)

Mouse Anti-CDK5RAP2 Recombinant Antibody (CL3392)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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