Rabbit Anti-CDK5RAP2 Recombinant Antibody (EG609) (CBMAB-EN734-LY)

Name: Rabbit Anti-CDK5RAP2 Recombinant Antibody (EG609)
SKU: CBMAB-EN734-LY
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Rabbit

Clone

EG609

Application

WB, IF

Immunogen

Protein corresponding to human CDK5RAP2 (aa1-aa300).

Host Species

Rabbit

Specificity

Human

Antibody Isotype

IgG

Clonality

Monoclonal Antibody

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1-2 µg/ml
IF(ICC)	2 µg/ml

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, pH 7.4

Preservative

0.09% sodium azide

Concentration

0.5 mg/ml

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

CDK5 Regulatory Subunit Associated Protein 2

Introduction

CDK5RAP2 (CDK5 Regulatory Subunit Associated Protein 2) is a Protein Coding gene. Diseases associated with CDK5RAP2 include Microcephaly 3, Primary, Autosomal Recessive and Autosomal Recessive Primary Microcephaly. Among its related pathways are Organelle biogenesis and maintenance and Regulation of PLK1 Activity at G2/M Transition. Gene Ontology (GO) annotations related to this gene include protein kinase binding and transcription regulatory region DNA binding. An important paralog of this gene is PDE4DIP.

Entrez Gene ID

55755

UniProt ID

Q96JB5

Alternative Names

CDK5 Regulatory Subunit Associated Protein 2; CDK5 Activator-Binding Protein C48; Centrosomin; Cep215; CDK5 Regulatory Subunit-Associated Protein 2; Microcephaly, Primary Autosomal Recessive 3;

Function

Potential regulator of CDK5 activity via its interaction with CDK5R1. Negative regulator of centriole disengagement (licensing) which maintains centriole engagement and cohesion. Involved in regulation of mitotic spindle orientation (By similarity).
Plays a role in the spindle checkpoint activation by acting as a transcriptional regulator of both BUBR1 and MAD2 promoter. Together with EB1/MAPRE1, may promote microtubule polymerization, bundle formation, growth and dynamics at the plus ends. Regulates centrosomal maturation by recruitment of the gamma-tubulin ring complex (gamma-TuRC) onto centrosomes (PubMed:26485573).
In complex with PDE4DIP isoform 13/MMG8/SMYLE, MAPRE1 and AKAP9, contributes to microtubules nucleation and extension from the centrosome to the cell periphery (PubMed:29162697).
Required for the recruitment of AKAP9 to centrosomes (PubMed:29162697).
Plays a role in neurogenesis (By similarity).

Biological Process

Brain development Source: UniProtKB
Centriole replication Source: UniProtKB
Centrosome cycle Source: UniProtKB
Chromosome segregation Source: UniProtKB
Ciliary basal body-plasma membrane docking Source: Reactome
Establishment of mitotic spindle orientation Source: UniProtKB
G2/M transition of mitotic cell cycle Source: Reactome
Microtubule bundle formation Source: UniProtKB
Microtubule cytoskeleton organization Source: UniProtKB
Microtubule organizing center organization Source: UniProtKB
Negative regulation of centriole replication Source: UniProtKB
Negative regulation of neuron differentiation Source: Ensembl
Neurogenesis Source: UniProtKB
Positive regulation of microtubule polymerization Source: UniProtKB
Positive regulation of transcription, DNA-templated Source: UniProtKB
Regulation of G2/M transition of mitotic cell cycle Source: Reactome
Regulation of mitotic cell cycle spindle assembly checkpoint Source: UniProtKB
Regulation of neuron differentiation Source: UniProtKB

Cellular Location

Cytoplasm; Golgi apparatus; Centrosome; Cytoskeleton. Found in the pericentriolar region adhering to the surface of the centrosome and in the region of the centrosomal appendages. Localizes to microtubule plus ends in the presence of EB1/MAPRE1. Localization to centrosomes versus Golgi apparatus may be cell type-dependent. For instance, in SK-BR-3 and HEK293F cells, localizes to centrosomes but not to the Golgi apparatus (PubMed:29162697).

Involvement in disease

Microcephaly 3, primary, autosomal recessive (MCPH3): A disease defined as a head circumference more than 3 standard deviations below the age-related mean. Brain weight is markedly reduced and the cerebral cortex is disproportionately small. Despite this marked reduction in size, the gyral pattern is relatively well preserved, with no major abnormality in cortical architecture. Affected individuals are mentally retarded. Primary microcephaly is further defined by the absence of other syndromic features or significant neurological deficits due to degenerative brain disorder.

PTM

Phosphorylated in vitro by CDK5.

Wang, X., Rosales, J. L., Gao, X., & Lee, K. Y. (2021). Centromeric chromatin integrity is compromised by loss of Cdk5rap2, a transcriptional activator of CENP-A. Biomedicine & Pharmacotherapy, 138, 111463.

Ayed, I. B., Bouchaala, W., Bouzid, A., Feki, W., Souissi, A., Nsir, S. B., ... & Triki, C. (2021). Further insights into the spectrum phenotype of TRAPPC9 and CDK5RAP2 genes, segregating independently in a large Tunisian family with intellectual disability and microcephaly. European journal of medical genetics, 104373.

Wang, X. (2020). Role of Cdk5rap2 in Cell Senescence and CENP-A-mediated Centromeric Chromatin Integrity.

Alfares, A., Alhufayti, I., Alsubaie, L., Alowain, M., Almass, R., Alfadhel, M., ... & Eyaid, W. (2018). A new association between CDK5RAP2 microcephaly and congenital cataracts. Annals of human genetics, 82(3), 165-170.

Fong, K. W., Au, F. K., Jia, Y., Yang, S., Zhou, L., & Qi, R. Z. (2017). Microtubule plus-end tracking of end-binding protein 1 (EB1) is regulated by CDK5 regulatory subunit-associated protein 2. Journal of Biological Chemistry, 292(18), 7675-7687.

Zaqout, S. (2017). Cyclin-dependent kinase 5 regulatory subunit-associated protein 2 (Cdk5rap2) in neuronal differentiation and germ cell pool maintenance (Doctoral dissertation).

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Isotype control

For research use only. Not intended for any clinical use.

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