Human CDK5RAP2 ELISA Kit (V2LY-0626-LY4390)

Name: Human CDK5RAP2 ELISA Kit
SKU: V2LY-0626-LY4390
Rating: 5 (1 reviews)

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Tested Data

Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Sensitivity

0.0052 ng/mL

Detection Range

0.01-2 ng/mL

Sample Type

Serum, Plasma, cell culture supernates

Specificity

Human

Assay Type

Sandwich

Reactivity

Human

Assay Time

1.5 h

Molecule Mass

215.0 kDa

Components

Pre-coated ELISA plate: 12 wells * 8 detachable strips
Standard solution: 0.5ml x1
Standard diluent: 3ml x1
Streptavidin-HRP: 6ml x1
Stop solution: 6ml x1
Substrate solution A: 6ml x1
Substrate solution B: 6ml x1
Wash buffer concentrate (25x): 20ml x1
Biotinylated antibody: 1ml x1

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage

Store at 2-8°C

More Infomation

Target

Full Name

CDK5 Regulatory Subunit Associated Protein 2

Function

Potential regulator of CDK5 activity via its interaction with CDK5R1. Negative regulator of centriole disengagement (licensing) which maintains centriole engagement and cohesion. Involved in regulation of mitotic spindle orientation (By similarity).
Plays a role in the spindle checkpoint activation by acting as a transcriptional regulator of both BUBR1 and MAD2 promoter. Together with EB1/MAPRE1, may promote microtubule polymerization, bundle formation, growth and dynamics at the plus ends. Regulates centrosomal maturation by recruitment of the gamma-tubulin ring complex (gamma-TuRC) onto centrosomes (PubMed:26485573).
In complex with PDE4DIP isoform 13/MMG8/SMYLE, MAPRE1 and AKAP9, contributes to microtubules nucleation and extension from the centrosome to the cell periphery (PubMed:29162697).
Required for the recruitment of AKAP9 to centrosomes (PubMed:29162697).
Plays a role in neurogenesis (By similarity).

Biological Process

Brain development Source: UniProtKB
Centriole replication Source: UniProtKB
Centrosome cycle Source: UniProtKB
Chromosome segregation Source: UniProtKB
Ciliary basal body-plasma membrane docking Source: Reactome
Establishment of mitotic spindle orientation Source: UniProtKB
G2/M transition of mitotic cell cycle Source: Reactome
Microtubule bundle formation Source: UniProtKB
Microtubule cytoskeleton organization Source: UniProtKB
Microtubule organizing center organization Source: UniProtKB
Negative regulation of centriole replication Source: UniProtKB
Negative regulation of neuron differentiation Source: Ensembl
Neurogenesis Source: UniProtKB
Positive regulation of microtubule polymerization Source: UniProtKB
Positive regulation of transcription, DNA-templated Source: UniProtKB
Regulation of G2/M transition of mitotic cell cycle Source: Reactome
Regulation of mitotic cell cycle spindle assembly checkpoint Source: UniProtKB
Regulation of neuron differentiation Source: UniProtKB

Cellular Location

Cytoplasm; Golgi apparatus; Centrosome; Cytoskeleton. Found in the pericentriolar region adhering to the surface of the centrosome and in the region of the centrosomal appendages. Localizes to microtubule plus ends in the presence of EB1/MAPRE1. Localization to centrosomes versus Golgi apparatus may be cell type-dependent. For instance, in SK-BR-3 and HEK293F cells, localizes to centrosomes but not to the Golgi apparatus (PubMed:29162697).

Involvement in disease

Microcephaly 3, primary, autosomal recessive (MCPH3): A disease defined as a head circumference more than 3 standard deviations below the age-related mean. Brain weight is markedly reduced and the cerebral cortex is disproportionately small. Despite this marked reduction in size, the gyral pattern is relatively well preserved, with no major abnormality in cortical architecture. Affected individuals are mentally retarded. Primary microcephaly is further defined by the absence of other syndromic features or significant neurological deficits due to degenerative brain disorder.

PTM

Phosphorylated in vitro by CDK5.

Wang, X., Rosales, J. L., Gao, X., & Lee, K. Y. (2021). Centromeric chromatin integrity is compromised by loss of Cdk5rap2, a transcriptional activator of CENP-A. Biomedicine & Pharmacotherapy, 138, 111463.

Ayed, I. B., Bouchaala, W., Bouzid, A., Feki, W., Souissi, A., Nsir, S. B., ... & Triki, C. (2021). Further insights into the spectrum phenotype of TRAPPC9 and CDK5RAP2 genes, segregating independently in a large Tunisian family with intellectual disability and microcephaly. European journal of medical genetics, 104373.

Wang, X. (2020). Role of Cdk5rap2 in Cell Senescence and CENP-A-mediated Centromeric Chromatin Integrity.

Alfares, A., Alhufayti, I., Alsubaie, L., Alowain, M., Almass, R., Alfadhel, M., ... & Eyaid, W. (2018). A new association between CDK5RAP2 microcephaly and congenital cataracts. Annals of human genetics, 82(3), 165-170.

Fong, K. W., Au, F. K., Jia, Y., Yang, S., Zhou, L., & Qi, R. Z. (2017). Microtubule plus-end tracking of end-binding protein 1 (EB1) is regulated by CDK5 regulatory subunit-associated protein 2. Journal of Biological Chemistry, 292(18), 7675-7687.

Zaqout, S. (2017). Cyclin-dependent kinase 5 regulatory subunit-associated protein 2 (Cdk5rap2) in neuronal differentiation and germ cell pool maintenance (Doctoral dissertation).

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

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