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Mouse Anti-CLDN2 Recombinant Antibody (12H12) (CBMAB-C2661-LY)

This product is antibody recognizes CLDN2. The antibody 12H12 immunoassay techniques such as: IF, IHC-P, IP, WB.
See all CLDN2 antibodies
Published Data
Fig.1 rEhCP112a alters claudin-2 localization.
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Fig.2 rEhCP112a degrades claudin-1 and increases claudin-2 expression in Caco-2 cells.
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Fig.3 Expression of claudin-2, as determined by IF, in a fraction of cells within a primary CAF culture (passage 4).
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Summary

Host Animal
Mouse
Specificity
Mouse, Dog, Human, Rat
Clone
12H12
Antibody Isotype
IgG2b
Application
IF, IHC-P, IP, WB

Basic Information

Immunogen
Synthetic peptide corresponding to a 26 amino acid sequence at the C-terminus of mouse Claudin-2
Specificity
Mouse, Dog, Human, Rat
Antibody Isotype
IgG2b
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Preservative
0.1% sodium azide
Concentration
0.5 mg/ml
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
Claudin 2
Introduction
This gene product belongs to the claudin protein family whose members have been identified as major integral membrane proteins localized exclusively at tight junctions. Claudins are expressed in an organ-specific manner and regulate tissue-specific physiologic properties of tight junctions. This protein is expressed in the intestine. Alternatively spliced transcript variants with different 5' untranslated region have been found for this gene.[provided by RefSeq, Jan 2010]
Entrez Gene ID
Human9075
Mouse12738
Rat300920
Dog403649
UniProt ID
HumanP56856
MouseP56857
RatQ5I0E5
DogA0A5F4CHQ9
Function
Plays a major role in tight junction-specific obliteration of the intercellular space, through calcium-independent cell-adhesion activity.
Biological Process
Bicellular tight junction assembly Source: GO_Central
Calcium-independent cell-cell adhesion via plasma membrane cell-adhesion molecules Source: UniProtKB
Cell adhesion Source: GO_Central
Cellular Location
Cell membrane; Tight junction
Topology
Cytoplasmic: 1-7
Helical: 8-28
Extracellular: 29-81
Helical: 82-102
Cytoplasmic: 103-116
Helical: 117-137
Extracellular: 138-162
Helical: 163-183
Cytoplasmic: 184-230
PTM
The disulfide bond is necessary for pore formation, but is not required for correct protein trafficking.

Ganapathy, A. S., Saha, K., Suchanec, E., Singh, V., Verma, A., Yochum, G., ... & Nighot, P. (2021). AP2M1 mediates autophagy-induced CLDN2 (claudin 2) degradation through endocytosis and interaction with LC3 and reduces intestinal epithelial tight junction permeability. Autophagy, 1-18.

Eguchi, H., Matsunaga, H., Onuma, S., Yoshino, Y., Matsunaga, T., & Ikari, A. (2021). Down-regulation of claudin-2 expression by cyanidin-3-glucoside enhances sensitivity to anticancer drugs in the spheroid of human lung adenocarcinoma A549 cells. International Journal of Molecular Sciences, 22(2), 499.

Beggs, M. R., Young, K., Pan, W., O’Neill, D. D., Saurette, M., Plain, A., ... & Alexander, R. T. (2021). Claudin-2 and claudin-12 form independent, complementary pores required to maintain calcium homeostasis. Proceedings of the National Academy of Sciences, 118(48).

Nasako, H., Akizuki, R., Takashina, Y., Ishikawa, Y., Shinoda, T., Shirouzu, M., ... & Ikari, A. (2020). Claudin-2 binding peptides, VPDSM and DSMKF, down-regulate claudin-2 expression and anticancer resistance in human lung adenocarcinoma A549 cells. Biochimica et Biophysica Acta (BBA)-Molecular Cell Research, 1867(4), 118642.

Okada, T., Konno, T., Kohno, T., Shimada, H., Saito, K., Satohisa, S., ... & Kojima, T. (2020). Possibility of targeting claudin-2 in therapy for human endometrioid endometrial carcinoma. Reproductive Sciences, 27(11), 2092-2103.

Raju, P., Shashikanth, N., Tsai, P. Y., Pongkorpsakol, P., Chanez-Paredes, S., Steinhagen, P. R., ... & Turner, J. R. (2020). Inactivation of paracellular cation-selective claudin-2 channels attenuates immune-mediated experimental colitis in mice. The Journal of Clinical Investigation, 130(10).

Eguchi, H., Matsunaga, T., Endo, S., Ichihara, K., & Ikari, A. (2020). Kaempferide Enhances Chemosensitivity of Human Lung Adenocarcinoma A549 Cells Mediated by the Decrease in Phosphorylation of Akt and Claudin-2 Expression. Nutrients, 12(4), 1190.

Dan, Q., Shi, Y., Rabani, R., Venugopal, S., Xiao, J., Anwer, S., ... & Szászi, K. (2019). Claudin-2 suppresses GEF-H1, RHOA, and MRTF, thereby impacting proliferation and profibrotic phenotype of tubular cells. Journal of Biological Chemistry, 294(42), 15446-15465.

Amoozadeh, Y., Anwer, S., Dan, Q., Venugopal, S., Shi, Y., Branchard, E., ... & Szaszi, K. (2018). Cell confluence regulates claudin-2 expression: possible role for ZO-1 and Rac. American Journal of Physiology-Cell Physiology, 314(3), C366-C378.

Takigawa, M., Iida, M., Nagase, S., Suzuki, H., Watari, A., Tada, M., ... & Kondoh, M. (2017). Creation of a Claudin-2 Binder and Its Tight Junction–Modulating Activity in a Human Intestinal Model. Journal of Pharmacology and Experimental Therapeutics, 363(3), 444-451.

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For research use only. Not intended for any clinical use.

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