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Mouse Anti-CLDN2 Recombinant Antibody (CBYY-C3151) (CBMAB-C4594-YY)

This product is mouse antibody that recognizes CLDN2. The antibody CBYY-C3151 can be used for immunoassay techniques such as: ELISA, WB
See all CLDN2 antibodies

Summary

Host Animal
Mouse
Specificity
Human
Clone
CBYY-C3151
Antibody Isotype
IgG2a, κ
Application
ELISA, WB

Basic Information

Immunogen
Partial recombinant corresponding to aa29-81 from CLDN2 (NP_065117) with GST tag. MW of the GST tag alone is 26kD
Specificity
Human
Antibody Isotype
IgG2a, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Protein A purified
Preservative
PBS, pH 7.2
Concentration
Liquid
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
Claudin 2
Entrez Gene ID
9075
UniProt ID
P57739
Alternative Names
Clusterin; Testosterone-Repressed Prostate Message 2; Apolipoprotein J; Complement-Associated Protein SP-40,40; Complement Cytolysis Inhibitor; Complement Lysis Inhibitor; Sulfated Glycoprotein 2; Ku70-Binding Protein 1; NA1/NA2; TRPM-2; APO-J; APOJ; KUB1;
Function
Plays a major role in tight junction-specific obliteration of the intercellular space, through calcium-independent cell-adhesion activity.
Biological Process
Bicellular tight junction assembly Source: GO_Central
Calcium-independent cell-cell adhesion via plasma membrane cell-adhesion molecules Source: UniProtKB
Cell adhesion Source: GO_Central
Cellular Location
Cell membrane; Tight junction
Topology
Cytoplasmic: 1-7
Helical: 8-28
Extracellular: 29-81
Helical: 82-102
Cytoplasmic: 103-116
Helical: 117-137
Extracellular: 138-162
Helical: 163-183
Cytoplasmic: 184-230
PTM
The disulfide bond is necessary for pore formation, but is not required for correct protein trafficking.

Ganapathy, A. S., Saha, K., Suchanec, E., Singh, V., Verma, A., Yochum, G., ... & Nighot, P. (2021). AP2M1 mediates autophagy-induced CLDN2 (claudin 2) degradation through endocytosis and interaction with LC3 and reduces intestinal epithelial tight junction permeability. Autophagy, 1-18.

Eguchi, H., Matsunaga, H., Onuma, S., Yoshino, Y., Matsunaga, T., & Ikari, A. (2021). Down-regulation of claudin-2 expression by cyanidin-3-glucoside enhances sensitivity to anticancer drugs in the spheroid of human lung adenocarcinoma A549 cells. International Journal of Molecular Sciences, 22(2), 499.

Beggs, M. R., Young, K., Pan, W., O’Neill, D. D., Saurette, M., Plain, A., ... & Alexander, R. T. (2021). Claudin-2 and claudin-12 form independent, complementary pores required to maintain calcium homeostasis. Proceedings of the National Academy of Sciences, 118(48).

Nasako, H., Akizuki, R., Takashina, Y., Ishikawa, Y., Shinoda, T., Shirouzu, M., ... & Ikari, A. (2020). Claudin-2 binding peptides, VPDSM and DSMKF, down-regulate claudin-2 expression and anticancer resistance in human lung adenocarcinoma A549 cells. Biochimica et Biophysica Acta (BBA)-Molecular Cell Research, 1867(4), 118642.

Okada, T., Konno, T., Kohno, T., Shimada, H., Saito, K., Satohisa, S., ... & Kojima, T. (2020). Possibility of targeting claudin-2 in therapy for human endometrioid endometrial carcinoma. Reproductive Sciences, 27(11), 2092-2103.

Raju, P., Shashikanth, N., Tsai, P. Y., Pongkorpsakol, P., Chanez-Paredes, S., Steinhagen, P. R., ... & Turner, J. R. (2020). Inactivation of paracellular cation-selective claudin-2 channels attenuates immune-mediated experimental colitis in mice. The Journal of Clinical Investigation, 130(10).

Eguchi, H., Matsunaga, T., Endo, S., Ichihara, K., & Ikari, A. (2020). Kaempferide Enhances Chemosensitivity of Human Lung Adenocarcinoma A549 Cells Mediated by the Decrease in Phosphorylation of Akt and Claudin-2 Expression. Nutrients, 12(4), 1190.

Dan, Q., Shi, Y., Rabani, R., Venugopal, S., Xiao, J., Anwer, S., ... & Szászi, K. (2019). Claudin-2 suppresses GEF-H1, RHOA, and MRTF, thereby impacting proliferation and profibrotic phenotype of tubular cells. Journal of Biological Chemistry, 294(42), 15446-15465.

Amoozadeh, Y., Anwer, S., Dan, Q., Venugopal, S., Shi, Y., Branchard, E., ... & Szaszi, K. (2018). Cell confluence regulates claudin-2 expression: possible role for ZO-1 and Rac. American Journal of Physiology-Cell Physiology, 314(3), C366-C378.

Takigawa, M., Iida, M., Nagase, S., Suzuki, H., Watari, A., Tada, M., ... & Kondoh, M. (2017). Creation of a Claudin-2 Binder and Its Tight Junction–Modulating Activity in a Human Intestinal Model. Journal of Pharmacology and Experimental Therapeutics, 363(3), 444-451.

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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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