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Mouse Anti-DCLK1 Recombinant Antibody (7D10) (CBMAB-A2102-LY)

The product is antibody recognizes DCAMKL1. The antibody 7D10 immunoassay techniques such as: WB, ELISA.
See all DCLK1 antibodies

Summary

Host Animal
Mouse
Specificity
Human, Mouse
Clone
7D10
Antibody Isotype
IgG1, κ
Application
WB, ELISA

Basic Information

Immunogen
DCAMKL1 (NP_004725, 640 a.a. ~ 729 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Specificity
Human, Mouse
Antibody Isotype
IgG1, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Purity
> 95% Purity determined by SDS-PAGE.
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
Doublecortin Like Kinase 1
Introduction
DCAMKL1 is a microtubule-associated protein that phosphorylates itself and myelin basic protein (MBP; MIM 159430). DCAMKL1 has microtubule polymerizing activity that is independent of its protein kinase activity (Lin et al., 2000 [PubMed 11124993]).[supplied by OMIM
Entrez Gene ID
Human9201
Mouse13175
UniProt ID
HumanO15075
MouseQ9JLM8
Alternative Names
DCAMKL1; DCDC3A; DCLK; KIAA0369
Function
Probable kinase that may be involved in a calcium-signaling pathway controlling neuronal migration in the developing brain. May also participate in functions of the mature nervous system.
Biological Process
Axon extension Source: Ensembl
Central nervous system development Source: ProtInc
Central nervous system projection neuron axonogenesis Source: Ensembl
Dendrite morphogenesis Source: Ensembl
Endosomal transport Source: UniProtKB
Forebrain development Source: Ensembl
Intracellular signal transduction Source: InterPro
Negative regulation of protein localization to nucleus Source: Ensembl
Nervous system development Source: ProtInc
Neuron migration Source: Ensembl
Neuron projection morphogenesis Source: GO_Central
Peptidyl-serine phosphorylation Source: Ensembl
Protein phosphorylation Source: ProtInc
Response to virus Source: UniProtKB
Cellular Location
Integral component of plasma membrane; Postsynaptic density

Patel, O., Roy, M. J., Kropp, A., Hardy, J. M., Dai, W., & Lucet, I. S. (2021). Structural basis for small molecule targeting of Doublecortin Like Kinase 1 with DCLK1-IN-1. Communications biology, 4(1), 1-13.

Agulto, R. L., Rogers, M. M., Tan, T. C., Ramkumar, A., Downing, A. M., Bodin, H., ... & Ori-McKenney, K. M. (2021). Autoregulatory control of microtubule binding in doublecortin-like kinase 1. Elife, 10, e60126.

Onisha, P., Roy, M. J., Ashleigh, K., Hardy, J. M., Weiwen, D., & Lucet, I. S. (2021). Structural basis for small molecule targeting of Doublecortin Like Kinase 1 with DCLK1-IN-1. Communications Biology, 4(1).

Ferguson, F. M., Nabet, B., Raghavan, S., Liu, Y., Leggett, A. L., Kuljanin, M., ... & Gray, N. S. (2020). Discovery of a selective inhibitor of doublecortin like kinase 1. Nature chemical biology, 16(6), 635-643.

Vázquez-Vélez, G. E., Gonzales, K. A., Revelli, J. P., Adamski, C. J., Naini, F. A., Bajić, A., ... & Zoghbi, H. Y. (2020). Doublecortin-like kinase 1 regulates α-synuclein levels and toxicity. Journal of Neuroscience, 40(2), 459-477.

Wang, J., Wang, S., Zhou, J., & Qian, Q. (2018). miR-424-5p regulates cell proliferation, migration and invasion by targeting doublecortin-like kinase 1 in basal-like breast cancer. Biomedicine & Pharmacotherapy, 102, 147-152.

Li, J., Wang, Y., Ge, J., Li, W., Yin, L., Zhao, Z., ... & Wang, H. (2018). Doublecortin-like kinase 1 (DCLK1) regulates B cell-specific moloney murine leukemia virus insertion site 1 (Bmi-1) and is associated with metastasis and prognosis in pancreatic cancer. Cellular Physiology and Biochemistry, 51(1), 262-277.

Fan, M., Qian, N., & Dai, G. (2017). Expression and prognostic significance of doublecortin‑like kinase 1 in patients with hepatocellular carcinoma. Oncology Letters, 14(6), 7529-7537.

Westphalen, C. B., Quante, M., & Wang, T. C. (2017). Functional implication of Dclk1 and Dclk1-expressing cells in cancer. Small GTPases, 8(3), 164-171.

Wu, X., Ruan, Y., Jiang, H., & Xu, C. (2017). MicroRNA-424 inhibits cell migration, invasion, and epithelial mesenchymal transition by downregulating doublecortin-like kinase 1 in ovarian clear cell carcinoma. The International Journal of Biochemistry & Cell Biology, 85, 66-74.

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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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