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Mouse Anti-DCK Recombinant Antibody (2F10) (CBMAB-D0346-YC)

Provided herein is a Mouse monoclonal antibody, which binds to Deoxycytidine Kinase (DCK). The antibody can be used for immunoassay techniques, such as ELISA, ICC, IF, WB.
See all DCK antibodies

Summary

Host Animal
Mouse
Specificity
Human
Clone
2F10
Antibody Isotype
IgG2b, κ
Application
ELISA, ICC, IF, WB

Basic Information

Immunogen
DCK (NP_000779, 161 a.a. ~ 260 a.a) partial recombinant protein with GST tag.
Specificity
Human
Antibody Isotype
IgG2b, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
deoxycytidine kinase
Introduction
DCK is required for the phosphorylation of several deoxyribonucleosides and their nucleoside analogs. Deficiency of DCK is associated with resistance to antiviral and anticancer chemotherapeutic agents. Conversely, increased deoxycytidine kinase activity is associated with increased activation of these compounds to cytotoxic nucleoside triphosphate derivatives. DCK is clinically important because of its relationship to drug resistance and sensitivity.
Entrez Gene ID
1633
UniProt ID
P27707
Alternative Names
Deoxycytidine Kinase; EC 2.7.1.74; EC 2.7.1; DCK;
Function
Phosphorylates the deoxyribonucleosides deoxycytidine, deoxyguanosine and deoxyadenosine (PubMed:1996353, PubMed:12808445, PubMed:18377927, PubMed:19159229, PubMed:20614893).

Has broad substrate specificity, and does not display selectivity based on the chirality of the substrate. It is also an essential enzyme for the phosphorylation of numerous nucleoside analogs widely employed as antiviral and chemotherapeutic agents (PubMed:12808445).
Biological Process
Purine-containing compound salvage Source: Reactome
Pyrimidine nucleoside salvage Source: Reactome
Pyrimidine nucleotide metabolic process Source: UniProtKB
Cellular Location
Nucleus
PTM
Phosphorylated and activated in vitro upon phosphorylation at Ser-74 by CSNK1D/CK1.

Yoshida, K., Fujita, A., Narazaki, H., Asano, T., & Itoh, Y. (2022). Drug resistance to nelarabine in leukemia cell lines might be caused by reduced expression of deoxycytidine kinase through epigenetic mechanisms. Cancer chemotherapy and pharmacology, 89(1), 83-91.

Carlini, F., Ivaldi, F., Gualandi, F., Boschert, U., Centonze, D., Matarese, G., ... & Uccelli, A. (2021). Different susceptibility of T and B cells to cladribine depends on their levels of deoxycytidine kinase activity linked to activation status. Journal of Neuroimmune Pharmacology, 1-11.

Wu, B., Mao, Z. J., Wang, Z., Wu, P., Huang, H., Zhao, W., ... & Yin, B. (2021). Deoxycytidine kinase (DCK) mutations in human acute myeloid leukemia resistant to Cytarabine. Acta Haematologica, 144(5), 534-541.

Hosokawa, M., Seiki, R., Iwakawa, S., & Ogawara, K. I. (2021). Combination of azacytidine and curcumin is a potential alternative in decitabine-resistant colorectal cancer cells with attenuated deoxycytidine kinase. Biochemical and Biophysical Research Communications, 578, 157-162.

Jaramillo, A. C., Bergman, A. M., Comijn, E. M., Jansen, G., Kaspers, G. J., Cloos, J., & Peters, G. J. (2020). Effect of dexamethasone on the antileukemic effect of cytarabine: role of deoxycytidine kinase. Nucleosides, Nucleotides & Nucleic Acids, 39(10-12), 1346-1346.

Vena, F., Bayle, S., Nieto, A., Quereda, V., Aceti, M., Frydman, S. M., ... & Duckett, D. (2020). Targeting casein kinase 1 delta sensitizes pancreatic and bladder cancer cells to gemcitabine treatment by upregulating deoxycytidine kinase. Molecular cancer therapeutics, 19(8), 1623-1635.

Sun, X., Xu, X., Chen, Y., Guan, R., Cheng, T., Wang, Y., ... & Hang, T. (2019). Danggui buxue decoction sensitizes the response of non-small-cell lung cancer to gemcitabine via regulating deoxycytidine kinase and P-glycoprotein. Molecules, 24(10), 2011.

Han, Z., Li, Y., Zhang, J., Liu, J., Chen, C., Van Zijl, P. C., & Liu, G. (2019). Molecular imaging of deoxycytidine kinase activity using deoxycytidine-enhanced CEST MRI. Cancer research, 79(10), 2775-2783.

Hellendahl, K. F., Kamel, S., Wetterwald, A., Neubauer, P., & Wagner, A. (2019). Human deoxycytidine kinase is a valuable biocatalyst for the synthesis of nucleotide analogues. Catalysts, 9(12), 997.

Huang, M., Inukai, T., Miyake, K., Tanaka, Y., Kagami, K., Abe, M., ... & Sugita, K. (2018). Clofarabine exerts antileukemic activity against cytarabine‐resistant B‐cell precursor acute lymphoblastic leukemia with low deoxycytidine kinase expression. Cancer medicine, 7(4), 1297-1316.

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For research use only. Not intended for any clinical use.

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