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Mouse Anti-DCK Recombinant Antibody (CBYCD-179) (CBMAB-D0350-YC)

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Summary

Host Animal
Mouse
Specificity
Human, Mouse, Rat
Clone
CBYCD-179
Antibody Isotype
IgG2b
Application
WB, IP, IHC-P, IF

Basic Information

Immunogen
Recombinant protein encompassing a sequence within the center region of human DCK.
Host Species
Mouse
Specificity
Human, Mouse, Rat
Antibody Isotype
IgG2b
Clonality
Monoclonal Antibody
Application Notes
ApplicationNote
WB1:1,000-1:10,000
IP1:100-1:500
IF(ICC)1:100-1:1,000
IHC-P1:100-1:1,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS
Preservative
None
Concentration
Batch dependent

Target

Full Name
deoxycytidine kinase
Entrez Gene ID
UniProt ID
Function
Phosphorylates the deoxyribonucleosides deoxycytidine, deoxyguanosine and deoxyadenosine (PubMed:1996353, PubMed:12808445, PubMed:18377927, PubMed:19159229, PubMed:20614893).

Has broad substrate specificity, and does not display selectivity based on the chirality of the substrate. It is also an essential enzyme for the phosphorylation of numerous nucleoside analogs widely employed as antiviral and chemotherapeutic agents (PubMed:12808445).
Biological Process
Purine-containing compound salvage Source: Reactome
Pyrimidine nucleoside salvage Source: Reactome
Pyrimidine nucleotide metabolic process Source: UniProtKB
Cellular Location
Nucleus
PTM
Phosphorylated and activated in vitro upon phosphorylation at Ser-74 by CSNK1D/CK1.
More Infomation

Yoshida, K., Fujita, A., Narazaki, H., Asano, T., & Itoh, Y. (2022). Drug resistance to nelarabine in leukemia cell lines might be caused by reduced expression of deoxycytidine kinase through epigenetic mechanisms. Cancer chemotherapy and pharmacology, 89(1), 83-91.

Carlini, F., Ivaldi, F., Gualandi, F., Boschert, U., Centonze, D., Matarese, G., ... & Uccelli, A. (2021). Different susceptibility of T and B cells to cladribine depends on their levels of deoxycytidine kinase activity linked to activation status. Journal of Neuroimmune Pharmacology, 1-11.

Wu, B., Mao, Z. J., Wang, Z., Wu, P., Huang, H., Zhao, W., ... & Yin, B. (2021). Deoxycytidine kinase (DCK) mutations in human acute myeloid leukemia resistant to Cytarabine. Acta Haematologica, 144(5), 534-541.

Hosokawa, M., Seiki, R., Iwakawa, S., & Ogawara, K. I. (2021). Combination of azacytidine and curcumin is a potential alternative in decitabine-resistant colorectal cancer cells with attenuated deoxycytidine kinase. Biochemical and Biophysical Research Communications, 578, 157-162.

Jaramillo, A. C., Bergman, A. M., Comijn, E. M., Jansen, G., Kaspers, G. J., Cloos, J., & Peters, G. J. (2020). Effect of dexamethasone on the antileukemic effect of cytarabine: role of deoxycytidine kinase. Nucleosides, Nucleotides & Nucleic Acids, 39(10-12), 1346-1346.

Vena, F., Bayle, S., Nieto, A., Quereda, V., Aceti, M., Frydman, S. M., ... & Duckett, D. (2020). Targeting casein kinase 1 delta sensitizes pancreatic and bladder cancer cells to gemcitabine treatment by upregulating deoxycytidine kinase. Molecular cancer therapeutics, 19(8), 1623-1635.

Sun, X., Xu, X., Chen, Y., Guan, R., Cheng, T., Wang, Y., ... & Hang, T. (2019). Danggui buxue decoction sensitizes the response of non-small-cell lung cancer to gemcitabine via regulating deoxycytidine kinase and P-glycoprotein. Molecules, 24(10), 2011.

Han, Z., Li, Y., Zhang, J., Liu, J., Chen, C., Van Zijl, P. C., & Liu, G. (2019). Molecular imaging of deoxycytidine kinase activity using deoxycytidine-enhanced CEST MRI. Cancer research, 79(10), 2775-2783.

Hellendahl, K. F., Kamel, S., Wetterwald, A., Neubauer, P., & Wagner, A. (2019). Human deoxycytidine kinase is a valuable biocatalyst for the synthesis of nucleotide analogues. Catalysts, 9(12), 997.

Huang, M., Inukai, T., Miyake, K., Tanaka, Y., Kagami, K., Abe, M., ... & Sugita, K. (2018). Clofarabine exerts antileukemic activity against cytarabine‐resistant B‐cell precursor acute lymphoblastic leukemia with low deoxycytidine kinase expression. Cancer medicine, 7(4), 1297-1316.

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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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