Mouse Anti-DCK Recombinant Antibody (D0345) (CBMAB-D0345-YC)

Name: Mouse Anti-DCK Recombinant Antibody (D0345)
SKU: CBMAB-D0345-YC
Rating: 5 (1 reviews)

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Published Data

Mouse Anti-DCK Recombinant Antibody (D0345) (CBMAB-D0345-YC) in IHC

PDAC PDX models of acquired gemcitabine resistance harbored changes in expression of some gemcitabine transporters and metabolizing enzymes. Tumor tissue harvested on days 190, 120, and 179 for PA4.gemR, PA10.gemR and PA16.gemR, respectively, was immunostained for RRM1, RRM2, hCNT1, hENT1, CDA, and dCK. Tumor tissue harvested from control mice bearing gemcitabine-naïve PA4, PA10 or PA16 tumors served as controls for IHC staining experiments. Scale bar = 10 μm. ...View More

Miller, A. L., Garcia, P. L., Gamblin, T. L., Vance, R. B., & Yoon, K. J. (2020). Development of gemcitabine-resistant patient-derived xenograft models of pancreatic ductal adenocarcinoma. Cancer Drug Resistance, 3(3), 572. Distributed under Open Access license CC BY 4.0, without modification.

Mouse Anti-DCK Recombinant Antibody (D0345) (CBMAB-D0345-YC) in WB

Expression of P-gp and dCK protein (A,B,D), and mRNA (C,E) level in tumor tissue of LLC tumor-bearing mice. Data were expressed as mean ± SEM (n = 10) * p < 0.05, GEM + DBD versus GEM. ** p < 0.01, GEM + DBD versus GEM. ## p < 0.01, GEM versus control (Con). ### p < 0.001, GEM versus Con. ...View More

Sun, X., Xu, X., Chen, Y., Guan, R., Cheng, T., Wang, Y., ... & Hang, T. (2019). Danggui buxue decoction sensitizes the response of non-small-cell lung cancer to gemcitabine via regulating deoxycytidine kinase and P-glycoprotein. Molecules, 24(10), 2011. Distributed under Open Access license CC BY 4.0, without modification.

Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

D0345

Application

WB, IP, IF, ELISA, IHC-P

Immunogen

Amino acids 203-241 mapping near the C-terminus of human Dck.

Host Species

Mouse

Specificity

Human, Mouse, Rat, Cattle, Pig

Antibody Isotype

IgG1, κ

Clonality

Monoclonal Antibody

Application Notes

Application	Note
WB	1:100-1:1,000
IP	1-2 μg per 100-500 μg of total protein (1 mL of cell lysate)
IF(ICC)	1:50-1:500
ELISA	1:100-1:1,000
IHC-P	1:50-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, 0.1% gelatin

Preservative

< 0.1% sodium azide

Concentration

0.2 mg/ml

More Infomation

Target

Full Name

deoxycytidine kinase

Entrez Gene ID

1633

UniProt ID

P27707

Function

Phosphorylates the deoxyribonucleosides deoxycytidine, deoxyguanosine and deoxyadenosine (PubMed:1996353, PubMed:12808445, PubMed:18377927, PubMed:19159229, PubMed:20614893).

Has broad substrate specificity, and does not display selectivity based on the chirality of the substrate. It is also an essential enzyme for the phosphorylation of numerous nucleoside analogs widely employed as antiviral and chemotherapeutic agents (PubMed:12808445).

Biological Process

Purine-containing compound salvage Source: Reactome
Pyrimidine nucleoside salvage Source: Reactome
Pyrimidine nucleotide metabolic process Source: UniProtKB

Cellular Location

Nucleus

PTM

Phosphorylated and activated in vitro upon phosphorylation at Ser-74 by CSNK1D/CK1.

Yoshida, K., Fujita, A., Narazaki, H., Asano, T., & Itoh, Y. (2022). Drug resistance to nelarabine in leukemia cell lines might be caused by reduced expression of deoxycytidine kinase through epigenetic mechanisms. Cancer chemotherapy and pharmacology, 89(1), 83-91.

Carlini, F., Ivaldi, F., Gualandi, F., Boschert, U., Centonze, D., Matarese, G., ... & Uccelli, A. (2021). Different susceptibility of T and B cells to cladribine depends on their levels of deoxycytidine kinase activity linked to activation status. Journal of Neuroimmune Pharmacology, 1-11.

Wu, B., Mao, Z. J., Wang, Z., Wu, P., Huang, H., Zhao, W., ... & Yin, B. (2021). Deoxycytidine kinase (DCK) mutations in human acute myeloid leukemia resistant to Cytarabine. Acta Haematologica, 144(5), 534-541.

Hosokawa, M., Seiki, R., Iwakawa, S., & Ogawara, K. I. (2021). Combination of azacytidine and curcumin is a potential alternative in decitabine-resistant colorectal cancer cells with attenuated deoxycytidine kinase. Biochemical and Biophysical Research Communications, 578, 157-162.

Jaramillo, A. C., Bergman, A. M., Comijn, E. M., Jansen, G., Kaspers, G. J., Cloos, J., & Peters, G. J. (2020). Effect of dexamethasone on the antileukemic effect of cytarabine: role of deoxycytidine kinase. Nucleosides, Nucleotides & Nucleic Acids, 39(10-12), 1346-1346.

Vena, F., Bayle, S., Nieto, A., Quereda, V., Aceti, M., Frydman, S. M., ... & Duckett, D. (2020). Targeting casein kinase 1 delta sensitizes pancreatic and bladder cancer cells to gemcitabine treatment by upregulating deoxycytidine kinase. Molecular cancer therapeutics, 19(8), 1623-1635.

Han, Z., Li, Y., Zhang, J., Liu, J., Chen, C., Van Zijl, P. C., & Liu, G. (2019). Molecular imaging of deoxycytidine kinase activity using deoxycytidine-enhanced CEST MRI. Cancer research, 79(10), 2775-2783.

Hellendahl, K. F., Kamel, S., Wetterwald, A., Neubauer, P., & Wagner, A. (2019). Human deoxycytidine kinase is a valuable biocatalyst for the synthesis of nucleotide analogues. Catalysts, 9(12), 997.

Huang, M., Inukai, T., Miyake, K., Tanaka, Y., Kagami, K., Abe, M., ... & Sugita, K. (2018). Clofarabine exerts antileukemic activity against cytarabine‐resistant B‐cell precursor acute lymphoblastic leukemia with low deoxycytidine kinase expression. Cancer medicine, 7(4), 1297-1316.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

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For research use only. Not intended for any clinical use.

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