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Recombinant Mouse Anti-DEFA5 Antibody (CBHb274) (CBMAB-Hb426-LY)

The product is antibody recognizes DEFA5. The antibody CBHb274 immunoassay techniques such as: IHC-F, Immuno assays, WB.
See all DEFA5 antibodies

Summary

Host Animal
Mouse
Specificity
Human
Clone
CBHb274
Antibody Isotype
IgG2b
Application
IHC-F, Immuno assays, WB

Basic Information

Specificity
Human
Antibody Isotype
IgG2b
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
0.1% BSA
Preservative
0.02% sodium azide
Concentration
0.1 mg/ml
Purity
> 95% Purity determined by SDS-PAGE.
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
Defensin Alpha 5
Introduction
Defensins are a family of antimicrobial and cytotoxic peptides thought to be involved in host defense. They are abundant in the granules of neutrophils and also found in the epithelia of mucosal surfaces such as those of the intestine, respiratory tract, urinary tract, and vagina. Members of the defensin family are highly similar in protein sequence and distinguished by a conserved cysteine motif. Several of the alpha defensin genes appear to be clustered on chromosome 8. The protein encoded by this gene, defensin, alpha 5, is highly expressed in the secretory granules of Paneth cells of the ileum. [provided by RefSeq, Oct 2014]
Entrez Gene ID
1670
UniProt ID
Q01523
Alternative Names
Defensin Alpha 5; Defensin, Alpha 5, Paneth Cell-Specific; HD5(20-94); DEF5; Defensin, Alpha 5, Preproprotein; Defensin, Alpha 5; Defensin-5; HD-5;
Function
Has antimicrobial activity against Gram-negative and Gram-positive bacteria. Defensins are thought to kill microbes by permeabilizing their plasma membrane. All DEFA5 peptides exert antimicrobial activities, but their potency is affected by peptide processing.
Biological Process
Antibacterial humoral response Source: GO_Central
Antimicrobial humoral immune response mediated by antimicrobial peptide Source: GO_Central
Antimicrobial humoral response Source: Reactome
Cellular response to lipopolysaccharide Source: GO_Central
Defense response to fungus Source: UniProtKB-KW
Defense response to Gram-negative bacterium Source: UniProtKB
defense response to Gram-positive bacterium Source: UniProtKB
Innate immune response Source: UniProtKB
Innate immune response in mucosa Source: GO_Central
Killing by host of symbiont cells Source: CACAO
Killing of cells of other organism Source: UniProtKB
Membrane disruption in other organism Source: GO_Central
Positive regulation of membrane permeability Source: UniProtKB
Cellular Location
Secreted; Secretory vesicle. Peptides HD5(20-94), HD5(23-94) and HD5(29-94) are found within tissues, HD5(20-94) being the predominant intracellular form. Peptides HD5(56-94) and HD5(63-94) are found in the extracellular milieu, HD5(63-94) being the most abundant form.
PTM
Glycosylated.

Luan, J., Ren, Y., Gao, S., & Zhang, L. (2022). High level of defensin alpha 5 in intestine may explain the low incidence of diarrhoea in COVID-19 patients. European Journal of Gastroenterology & Hepatology.

Qiao, Q., Bai, R., Song, W., Gao, H., Zhang, M., Lu, J., ... & Zhao, P. (2021). Human α-defensin 5 suppressed colon cancer growth by targeting PI3K pathway. Experimental Cell Research, 407(2), 112809.

Rana, T., Korolkova, O. Y., Rachakonda, G., Williams, A. D., Hawkins, A. T., James, S. D., ... & M’Koma, A. E. (2021). Linking bacterial enterotoxins and alpha defensin 5 expansion in the Crohn’s colitis: A new insight into the etiopathogenetic and differentiation triggers driving colonic inflammatory bowel disease. Plos one, 16(3), e0246393.

Wu, Z., Ding, Z., Cheng, B., & Cui, Z. (2021). The inhibitory effect of human DEFA5 in growth of gastric cancer by targeting BMI1. Cancer science, 112(3), 1075-1083.

Larsen, I. S., Fritzen, A. M., Carl, C. S., Agerholm, M., Damgaard, M. T. F., Holm, J. B., ... & Jensen, B. A. H. (2019). Human Paneth cell α-defensin-5 treatment reverses dyslipidemia and improves glucoregulatory capacity in diet-induced obese mice. American Journal of Physiology-Endocrinology and Metabolism, 317(1), E42-E52.

Rashidi, A., Shanley, R., Holtan, S. G., MacMillan, M. L., Yohe, S., Thyagarajan, B., ... & Weisdorf, D. J. (2018). Single Nucleotide Polymorphisms of Paneth Cell Antimicrobial Alpha Defensin-5 (DEFA5) are Associated with Acute Graft-Versus-Host Disease: Results From Analysis of BMT-CTN0201 Samples. Biology of Blood and Marrow Transplantation, 24(3), S174-S175.

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For research use only. Not intended for any clinical use.

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