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Mouse Anti-EED (AA 342-441) Recombinant Antibody (CBFYE-0454) (CBMAB-E0788-FY)

This product is mouse antibody that recognizes EED. The antibody CBFYE-0454 can be used for immunoassay techniques such as: WB, IP, ELISA.
See all EED antibodies

Summary

Host Animal
Mouse
Specificity
Human
Clone
CBFYE-0454
Antibody Isotype
IgG2a, κ
Application
WB, IP, ELISA

Basic Information

Immunogen
EED partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Immunogen sequence: KIKPSESNVT ILGRFDYSQC DIWYMRFSMD FWQKMLALGN QVGKLYVWDL EVEDPHKAKC TTLTHHKCGA AIRQTSFSRD SSILIAVCDD ASIWRWDRLR
Specificity
Human
Antibody Isotype
IgG2a, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, pH 7.2
Concentration
0.1 mg/mL
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Epitope
AA 342-441

Target

Full Name
embryonic ectoderm development
Introduction
This gene encodes a member of the Polycomb-group (PcG) family. PcG family members form multimeric protein complexes, which are involved in maintaining the transcriptional repressive state of genes over successive cell generations. This protein interacts with enhancer of zeste 2, the cytoplasmic tail of integrin beta7, immunodeficiency virus type 1 (HIV-1) MA protein, and histone deacetylase proteins.
Entrez Gene ID
8726
UniProt ID
O75530
Alternative Names
HEED; COGIS; WAIT1
Research Area
Polycomb group (PcG) protein. Component of the PRC2/EED-EZH2 complex, which methylates 'Lys-9' and 'Lys-27' of histone H3, leading to transcriptional repression of the affected target gene. Also recognizes 'Lys-26' trimethylated histone H1 with the effect of inhibiting PRC2 complex methyltransferase activity on nucleosomal histone H3 'Lys-27', whereas H3 'Lys-27' recognition has the opposite effect, enabling the propagation of this repressive mark. The PRC2/EED-EZH2 complex may also serve as a recruiting platform for DNA methyltransferases, thereby linking two epigenetic repression systems. Genes repressed by the PRC2/EED-EZH2 complex include HOXC8, HOXA9, MYT1 and CDKN2A.
Biological Process
Chromatin silencing Source: GO_Central
Histone H3-K27 methylation Source: GOC
Negative regulation of transcription, DNA-templated Source: UniProtKB
Negative regulation of transcription by RNA polymerase II Source: GO_Central
Spinal cord development Source: Ensembl
Cellular Location
Nucleus; Chromosome. Transiently colocalizes with XIST at inactive X chromosomes.
Involvement in disease
Cohen-Gibson syndrome (COGIS):
An autosomal dominant overgrowth disorder characterized by accelerated osseous maturation, advanced bone age, skeletal abnormalities including flaring of the metaphyses of the long bones, large hands with long fingers and camptodactyly, scoliosis, cervical spine anomalies, dysmorphic facial features, and variable intellectual disability.
PTM
Methylated. Binding to histone H1 'Lys-26' promotes mono-, di-, and trimethylation of internal lysines.

Zhang, J. Y., Zhang, J., Kiffe, M., Walles, M., Jin, Y., Blanz, J., ... & Oyang, C. (2022). Preclinical pharmacokinetics and metabolism of MAK683, a clinical stage selective oral embryonic ectoderm development (EED) inhibitor for cancer treatment. Xenobiotica, 52(1), 65-78.

Zhang, F., Yan, Y., Cao, X., Zhang, J., Li, Y., & Guo, C. (2021). Methylation of microRNA-338-5p by EED promotes METTL3-mediated translation of oncogene CDCP1 in gastric cancer. Aging (Albany NY), 13(8), 12224.

Cook, N., Chen, J., Zhou, J., & Wu, D. (2021). Embryonic ectoderm development (EED) as a novel target for cancer treatment. Current topics in medicinal chemistry, 21(31), 2771-2777.

Rej, R. K., Wang, C., Lu, J., Wang, M., Petrunak, E., Zawacki, K. P., ... & Wang, S. (2021). Discovery of EEDi-5273 as an exceptionally potent and orally efficacious EED inhibitor capable of achieving complete and persistent tumor regression. Journal of medicinal chemistry, 64(19), 14540-14556.

Sozen, B., Demir, N., & Zernicka-Goetz, M. (2021). BMP signalling is required for extra-embryonic ectoderm development during pre-to-post-implantation transition of the mouse embryo. Developmental biology, 470, 84-94.

Du, D., Xu, D., Zhu, L., Stazi, G., Zwergel, C., Liu, Y., ... & Luo, C. (2021). Structure-guided development of small-molecule PRC2 inhibitors targeting EZH2–EED interaction. Journal of Medicinal Chemistry, 64(12), 8194-8207.

Wang, C., Rej, R. K., Lu, J., Wang, M., Harvey, K. P., Yang, C. Y., ... & Wang, S. (2020). Discovery of a highly potent, efficacious and orally active small-molecule inhibitor of embryonic ectoderm development (EED). Cancer Research, 80(16_Supplement), 1036-1036.

Rej, R. K., Wang, C., Lu, J., Wang, M., Petrunak, E., Zawacki, K. P., ... & Wang, S. (2020). EEDi-5285: An exceptionally potent, efficacious, and orally active small-molecule inhibitor of embryonic ectoderm development. Journal of medicinal chemistry, 63(13), 7252-7267.

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For research use only. Not intended for any clinical use.

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