Mouse Anti-EED Recombinant Antibody (3B12) (CBMAB-A2459-LY)

Name: Mouse Anti-EED Recombinant Antibody (3B12)
SKU: CBMAB-A2459-LY
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

3B12

Application

WB, ELISA, IP

Immunogen

Amino acids 342-441 of human EED.

Host Species

Mouse

Specificity

Human, Mouse, Rat

Antibody Isotype

IgG2a, κ

Clonality

Monoclonal Antibody

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
ELISA	1:100-1:1,000
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

Gelatin & PBS

Preservative

Sodium Azide

Concentration

0.1 mg/ml

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

embryonic ectoderm development

Introduction

This gene encodes a member of the Polycomb-group (PcG) family. PcG family members form multimeric protein complexes, which are involved in maintaining the transcriptional repressive state of genes over successive cell generations. This protein interacts with enhancer of zeste 2, the cytoplasmic tail of integrin beta7, immunodeficiency virus type 1 (HIV-1) MA protein, and histone deacetylase proteins. This protein mediates repression of gene activity through histone deacetylation, and may act as a specific regulator of integrin function. Two transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq]

Entrez Gene ID

Human	8726
Mouse	13626

UniProt ID

Human	O75530
Mouse	Q921E6

Alternative Names

HEED; WAIT1

Research Area

Polycomb group (PcG) protein. Component of the PRC2/EED-EZH2 complex, which methylates 'Lys-9' and 'Lys-27' of histone H3, leading to transcriptional repression of the affected target gene. Also recognizes 'Lys-26' trimethylated histone H1 with the effect of inhibiting PRC2 complex methyltransferase activity on nucleosomal histone H3 'Lys-27', whereas H3 'Lys-27' recognition has the opposite effect, enabling the propagation of this repressive mark. The PRC2/EED-EZH2 complex may also serve as a recruiting platform for DNA methyltransferases, thereby linking two epigenetic repression systems. Genes repressed by the PRC2/EED-EZH2 complex include HOXC8, HOXA9, MYT1 and CDKN2A.

Biological Process

Chromatin silencing Source: GO_Central
Histone H3-K27 methylation Source: GOC
Negative regulation of transcription, DNA-templated Source: UniProtKB
Negative regulation of transcription by RNA polymerase II Source: GO_Central
Spinal cord development Source: Ensembl

Cellular Location

Nucleus; Chromosome. Transiently colocalizes with XIST at inactive X chromosomes.

Involvement in disease

Cohen-Gibson syndrome (COGIS):
An autosomal dominant overgrowth disorder characterized by accelerated osseous maturation, advanced bone age, skeletal abnormalities including flaring of the metaphyses of the long bones, large hands with long fingers and camptodactyly, scoliosis, cervical spine anomalies, dysmorphic facial features, and variable intellectual disability.

PTM

Methylated. Binding to histone H1 'Lys-26' promotes mono-, di-, and trimethylation of internal lysines.

Zhang, J. Y., Zhang, J., Kiffe, M., Walles, M., Jin, Y., Blanz, J., ... & Oyang, C. (2022). Preclinical pharmacokinetics and metabolism of MAK683, a clinical stage selective oral embryonic ectoderm development (EED) inhibitor for cancer treatment. Xenobiotica, 52(1), 65-78.

Zhang, F., Yan, Y., Cao, X., Zhang, J., Li, Y., & Guo, C. (2021). Methylation of microRNA-338-5p by EED promotes METTL3-mediated translation of oncogene CDCP1 in gastric cancer. Aging (Albany NY), 13(8), 12224.

Cook, N., Chen, J., Zhou, J., & Wu, D. (2021). Embryonic ectoderm development (EED) as a novel target for cancer treatment. Current topics in medicinal chemistry, 21(31), 2771-2777.

Rej, R. K., Wang, C., Lu, J., Wang, M., Petrunak, E., Zawacki, K. P., ... & Wang, S. (2021). Discovery of EEDi-5273 as an exceptionally potent and orally efficacious EED inhibitor capable of achieving complete and persistent tumor regression. Journal of medicinal chemistry, 64(19), 14540-14556.

Sozen, B., Demir, N., & Zernicka-Goetz, M. (2021). BMP signalling is required for extra-embryonic ectoderm development during pre-to-post-implantation transition of the mouse embryo. Developmental biology, 470, 84-94.

Du, D., Xu, D., Zhu, L., Stazi, G., Zwergel, C., Liu, Y., ... & Luo, C. (2021). Structure-guided development of small-molecule PRC2 inhibitors targeting EZH2–EED interaction. Journal of Medicinal Chemistry, 64(12), 8194-8207.

Wang, C., Rej, R. K., Lu, J., Wang, M., Harvey, K. P., Yang, C. Y., ... & Wang, S. (2020). Discovery of a highly potent, efficacious and orally active small-molecule inhibitor of embryonic ectoderm development (EED). Cancer Research, 80(16_Supplement), 1036-1036.

Rej, R. K., Wang, C., Lu, J., Wang, M., Petrunak, E., Zawacki, K. P., ... & Wang, S. (2020). EEDi-5285: An exceptionally potent, efficacious, and orally active small-molecule inhibitor of embryonic ectoderm development. Journal of medicinal chemistry, 63(13), 7252-7267.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

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