Human Recombinant EED protein, GST Tag (V2LY-0526-LY3689)

Name: Human Recombinant EED protein, GST Tag
SKU: V2LY-0526-LY3689
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

Baculovirus-Insect Cells

Protein Species

Human

Tag

GST Tag

Protein Construction

This product is Human Recombinant EED protein, GST Tag consist of Amino Acid: 1-441 and predicts a molecular mass of 78 kDa.

Molecule Mass

78 kDa

Sequence

Amino Acid: 1-441

Species

Human

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

>93% as determined by SDS-PAGE

Endotoxin

Please contact us for more information.

Format

Lyophilized

Reconstitution

Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.

Buffer

Lyophilized from sterile PBS

Preservative

None

Storage

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

More Infomation

Target

Full Name

embryonic ectoderm development

Research Area

Polycomb group (PcG) protein. Component of the PRC2/EED-EZH2 complex, which methylates 'Lys-9' and 'Lys-27' of histone H3, leading to transcriptional repression of the affected target gene. Also recognizes 'Lys-26' trimethylated histone H1 with the effect of inhibiting PRC2 complex methyltransferase activity on nucleosomal histone H3 'Lys-27', whereas H3 'Lys-27' recognition has the opposite effect, enabling the propagation of this repressive mark. The PRC2/EED-EZH2 complex may also serve as a recruiting platform for DNA methyltransferases, thereby linking two epigenetic repression systems. Genes repressed by the PRC2/EED-EZH2 complex include HOXC8, HOXA9, MYT1 and CDKN2A.

Biological Process

Chromatin silencing Source: GO_Central
Histone H3-K27 methylation Source: GOC
Negative regulation of transcription, DNA-templated Source: UniProtKB
Negative regulation of transcription by RNA polymerase II Source: GO_Central
Spinal cord development Source: Ensembl

Cellular Location

Nucleus; Chromosome. Transiently colocalizes with XIST at inactive X chromosomes.

Involvement in disease

Cohen-Gibson syndrome (COGIS):
An autosomal dominant overgrowth disorder characterized by accelerated osseous maturation, advanced bone age, skeletal abnormalities including flaring of the metaphyses of the long bones, large hands with long fingers and camptodactyly, scoliosis, cervical spine anomalies, dysmorphic facial features, and variable intellectual disability.

PTM

Methylated. Binding to histone H1 'Lys-26' promotes mono-, di-, and trimethylation of internal lysines.

Zhang, J. Y., Zhang, J., Kiffe, M., Walles, M., Jin, Y., Blanz, J., ... & Oyang, C. (2022). Preclinical pharmacokinetics and metabolism of MAK683, a clinical stage selective oral embryonic ectoderm development (EED) inhibitor for cancer treatment. Xenobiotica, 52(1), 65-78.

Zhang, F., Yan, Y., Cao, X., Zhang, J., Li, Y., & Guo, C. (2021). Methylation of microRNA-338-5p by EED promotes METTL3-mediated translation of oncogene CDCP1 in gastric cancer. Aging (Albany NY), 13(8), 12224.

Cook, N., Chen, J., Zhou, J., & Wu, D. (2021). Embryonic ectoderm development (EED) as a novel target for cancer treatment. Current topics in medicinal chemistry, 21(31), 2771-2777.

Rej, R. K., Wang, C., Lu, J., Wang, M., Petrunak, E., Zawacki, K. P., ... & Wang, S. (2021). Discovery of EEDi-5273 as an exceptionally potent and orally efficacious EED inhibitor capable of achieving complete and persistent tumor regression. Journal of medicinal chemistry, 64(19), 14540-14556.

Sozen, B., Demir, N., & Zernicka-Goetz, M. (2021). BMP signalling is required for extra-embryonic ectoderm development during pre-to-post-implantation transition of the mouse embryo. Developmental biology, 470, 84-94.

Du, D., Xu, D., Zhu, L., Stazi, G., Zwergel, C., Liu, Y., ... & Luo, C. (2021). Structure-guided development of small-molecule PRC2 inhibitors targeting EZH2–EED interaction. Journal of Medicinal Chemistry, 64(12), 8194-8207.

Wang, C., Rej, R. K., Lu, J., Wang, M., Harvey, K. P., Yang, C. Y., ... & Wang, S. (2020). Discovery of a highly potent, efficacious and orally active small-molecule inhibitor of embryonic ectoderm development (EED). Cancer Research, 80(16_Supplement), 1036-1036.

Rej, R. K., Wang, C., Lu, J., Wang, M., Petrunak, E., Zawacki, K. P., ... & Wang, S. (2020). EEDi-5285: An exceptionally potent, efficacious, and orally active small-molecule inhibitor of embryonic ectoderm development. Journal of medicinal chemistry, 63(13), 7252-7267.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Human Recombinant EED protein, His Tag (CAT#: V2LY-0526-LY3688)

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Human Recombinant EED protein, His Tag (CAT#: V2LY-0526-LY3688)

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Isotype control

For research use only. Not intended for any clinical use.

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