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Mouse Anti-EED Recombinant Antibody (CBFYE-0453) (V2LY-0425-LY1433)


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Summary

Host Animal
Mouse
Specificity
Human, Mouse, Rat
Clone
CBFYE-0453
Antibody Isotype
IgG1
Application
WB, IHC-P, IF

Basic Information

Immunogen
Recombinant protein encompassing a sequence within the center region of Human EED.
Host Species
Mouse
Specificity
Human, Mouse, Rat
Antibody Isotype
IgG1
Clonality
Monoclonal Antibody
Application Notes
ApplicationNote
WB1:500-1:3,000
IF(ICC)1:100-1:1,000
IHC-P1:100-1:1,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS
Preservative
None
Concentration
Batch dependent
Purity
>95% as determined by analysis by SDS-PAGE
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
embryonic ectoderm development
Entrez Gene ID
Human8726
Mouse13626
UniProt ID
HumanO75530
MouseQ921E6
Research Area
Polycomb group (PcG) protein. Component of the PRC2/EED-EZH2 complex, which methylates 'Lys-9' and 'Lys-27' of histone H3, leading to transcriptional repression of the affected target gene. Also recognizes 'Lys-26' trimethylated histone H1 with the effect of inhibiting PRC2 complex methyltransferase activity on nucleosomal histone H3 'Lys-27', whereas H3 'Lys-27' recognition has the opposite effect, enabling the propagation of this repressive mark. The PRC2/EED-EZH2 complex may also serve as a recruiting platform for DNA methyltransferases, thereby linking two epigenetic repression systems. Genes repressed by the PRC2/EED-EZH2 complex include HOXC8, HOXA9, MYT1 and CDKN2A.
Biological Process
Chromatin silencing Source: GO_Central
Histone H3-K27 methylation Source: GOC
Negative regulation of transcription, DNA-templated Source: UniProtKB
Negative regulation of transcription by RNA polymerase II Source: GO_Central
Spinal cord development Source: Ensembl
Cellular Location
Nucleus; Chromosome. Transiently colocalizes with XIST at inactive X chromosomes.
Involvement in disease
Cohen-Gibson syndrome (COGIS):
An autosomal dominant overgrowth disorder characterized by accelerated osseous maturation, advanced bone age, skeletal abnormalities including flaring of the metaphyses of the long bones, large hands with long fingers and camptodactyly, scoliosis, cervical spine anomalies, dysmorphic facial features, and variable intellectual disability.
PTM
Methylated. Binding to histone H1 'Lys-26' promotes mono-, di-, and trimethylation of internal lysines.
More Infomation

Zhang, J. Y., Zhang, J., Kiffe, M., Walles, M., Jin, Y., Blanz, J., ... & Oyang, C. (2022). Preclinical pharmacokinetics and metabolism of MAK683, a clinical stage selective oral embryonic ectoderm development (EED) inhibitor for cancer treatment. Xenobiotica, 52(1), 65-78.

Zhang, F., Yan, Y., Cao, X., Zhang, J., Li, Y., & Guo, C. (2021). Methylation of microRNA-338-5p by EED promotes METTL3-mediated translation of oncogene CDCP1 in gastric cancer. Aging (Albany NY), 13(8), 12224.

Cook, N., Chen, J., Zhou, J., & Wu, D. (2021). Embryonic ectoderm development (EED) as a novel target for cancer treatment. Current topics in medicinal chemistry, 21(31), 2771-2777.

Rej, R. K., Wang, C., Lu, J., Wang, M., Petrunak, E., Zawacki, K. P., ... & Wang, S. (2021). Discovery of EEDi-5273 as an exceptionally potent and orally efficacious EED inhibitor capable of achieving complete and persistent tumor regression. Journal of medicinal chemistry, 64(19), 14540-14556.

Sozen, B., Demir, N., & Zernicka-Goetz, M. (2021). BMP signalling is required for extra-embryonic ectoderm development during pre-to-post-implantation transition of the mouse embryo. Developmental biology, 470, 84-94.

Du, D., Xu, D., Zhu, L., Stazi, G., Zwergel, C., Liu, Y., ... & Luo, C. (2021). Structure-guided development of small-molecule PRC2 inhibitors targeting EZH2–EED interaction. Journal of Medicinal Chemistry, 64(12), 8194-8207.

Wang, C., Rej, R. K., Lu, J., Wang, M., Harvey, K. P., Yang, C. Y., ... & Wang, S. (2020). Discovery of a highly potent, efficacious and orally active small-molecule inhibitor of embryonic ectoderm development (EED). Cancer Research, 80(16_Supplement), 1036-1036.

Rej, R. K., Wang, C., Lu, J., Wang, M., Petrunak, E., Zawacki, K. P., ... & Wang, S. (2020). EEDi-5285: An exceptionally potent, efficacious, and orally active small-molecule inhibitor of embryonic ectoderm development. Journal of medicinal chemistry, 63(13), 7252-7267.

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For research use only. Not intended for any clinical use.

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