Mouse Anti-EFHD1 Recombinant Antibody (1A8) (CBMAB-A2485-LY)

The product is antibody recognizes EFHD1. The antibody 1A8 immunoassay techniques such as: WB, ELISA.
See all EFHD1 antibodies

Datasheet

Summary

Host Animal

Mouse

Specificity

Human, Mouse, Rat

Clone

1A8

Antibody Isotype

IgG2a, κ

Application

WB, ELISA

Basic Information

Immunogen

EFHD1 (NP_079478.1, 168 a.a. ~ 238 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Specificity

Human, Mouse, Rat

Antibody Isotype

IgG2a, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name

EF-Hand Domain Family Member D1

Introduction

EFHD1 is an EF-hand domain-containing protein that displays increased expression during neuronal differentiation (Tominaga and Tomooka, 2002 [PubMed 12270117]).[supplied by OMIM

Entrez Gene ID

Human	80303
Mouse	98363
Rat	501181

UniProt ID

Human	Q9BUP0
Mouse	Q9D4J1
Rat	D4A9T5

Alternative Names

DKFZp781H0842; FLJ13612; MST133; MSTP133; PP3051

Research Area

Acts as a calcium sensor for mitochondrial flash (mitoflash) activation, an event characterized by stochastic bursts of superoxide production (PubMed:26975899).

May play a role in neuronal differentiation (By similarity).

Biological Process

Neuron projection development Source: Ensembl
Regulation of cellular hyperosmotic salinity response Source: UniProtKB

Cellular Location

Mitochondrion inner membrane

References

Eberhardt, D. R., Lee, S. H., Yin, X., Balynas, A. M., Rekate, E. C., Kraiss, J. N., ... & Chaudhuri, D. (2022). EFHD1 ablation inhibits cardiac mitoflash activation and protects cardiomyocytes from ischemia. Journal of Molecular and Cellular Cardiology, 167, 1-14.

Eberhardt, D. R., Lee, S. H., Yin, X., Balynas, A. M., Rekate, E., Kraiss, J. N., ... & Chaudhuri, D. (2021). EFHD1 ablation reduces cardiac mitoflash activation and protects cardiomyocytes from ischemia. bioRxiv.

Eberhardt, D. R., Yin, X., Balynas, A., Lee, S., Walsh, M., Funai, K., & Chaudhuri, D. (2021, September). Abstract P339: Examining Cardiac Phenotypes Of EF Hand Containing Domain 1 Protein (EFHD1) Knockout Mice. In Circulation Research (Vol. 129, No. Suppl_1, pp. AP339-AP339). Hagerstown, MD: Lippincott Williams & Wilkins.

Mun, S. A., Park, J., Park, K. R., Lee, Y., Kang, J. Y., Park, T., ... & Eom, S. H. (2021). Structural and biochemical characterization of EFhd1/Swiprosin-2, an actin-binding protein in mitochondria. Frontiers in cell and developmental biology, 8, 628222.

Ulisse, V., Dey, S., Rothbard, D. E., Zeevi, E., Gokhman, I., Dadosh, T., ... & Yaron, A. (2020). Regulation of axonal morphogenesis by the mitochondrial protein Efhd1. Life science alliance, 3(7).

Q & As

Ask a question We look forward to hearing from you.

0 reviews or Q&As

Purchasing FAQs
Technical FAQs

Review & reward

Have you used Mouse Anti-EFHD1 Recombinant Antibody (1A8)?
Submit a review and get a Coupon or an Amazon gift card. 20% off Coupon

$30 eGift Card
Submit a review

Protocols

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Associated Products

Related Products

Mouse Anti-EFHD1 Recombinant Antibody (CB226A)

Mouse Anti-EFHD1 Recombinant Antibody (5K1)

Mouse Anti-EFHD1 (AA 168-238) Recombinant Antibody (CBFYE-0477)

Mouse Anti-EFHD1 Recombinant Antibody (CB181)

Mouse Anti-EFHD1 Recombinant Antibody (3D10)

Mouse Anti-EFHD1 Recombinant Antibody (CBXS-1028)

Mouse Anti-EFHD1 Recombinant Antibody (CAP666)

Mouse Anti-EFHD1 Recombinant Antibody (CAP667)

Mouse Anti-EFHD1 (AA 168-238) Recombinant Antibody (CBFYE-0036)

Mouse Anti-EFHD1 Recombinant Antibody (CBFYE-0476)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

Online Inquiry

Documents

Datasheet
SDS
Request for COA

Request bulk or custom quote

Second antibody and isotype control

Contact us

Tel: (USA)
(UK)
Fax:

Global Locations

Email: