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Mouse Anti-EIF4G2 Recombinant Antibody (3E4) (CBMAB-A2546-LY)

The product is antibody recognizes EIF4G2. The antibody 3E4 immunoassay techniques such as: WB, ELISA.
See all EIF4G2 antibodies

Summary

Host Animal
Mouse
Specificity
Human, Rat
Clone
3E4
Antibody Isotype
IgG1, κ
Application
WB, ELISA

Basic Information

Immunogen
EIF4G2 (NP_001409, 811 a.a. ~ 889 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Specificity
Human, Rat
Antibody Isotype
IgG1, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Purity
> 95% Purity determined by SDS-PAGE.
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
Eukaryotic Translation Initiation Factor 4 Gamma 2
Introduction
Translation initiation is mediated by specific recognition of the cap structure by eukaryotic translation initiation factor 4F (eIF4F), which is a cap binding protein complex that consists of three subunits: eIF4A, eIF4E and eIF4G. The protein encoded by this gene shares similarity with the C-terminal region of eIF4G that contains the binding sites for eIF4A and eIF3; eIF4G, in addition, contains a binding site for eIF4E at the N-terminus. Unlike eIF4G, which supports cap-dependent and independent translation, this gene product functions as a general repressor of translation by forming translationally inactive complexes. In vitro and in vivo studies indicate that translation of this mRNA initiates exclusively at a non-AUG (GUG) codon. Alternatively spliced transcript variants encoding different isoforms of this gene have been described. [provided by RefSeq]
Entrez Gene ID
Human1982
Rat361628
UniProt ID
HumanP78344
RatF1LN59
Alternative Names
AAG1; DAP5; FLJ41344; NAT1; p97
Research Area
Appears to play a role in the switch from cap-dependent to IRES-mediated translation during mitosis, apoptosis and viral infection. Cleaved by some caspases and viral proteases.
Biological Process
Cell death Source: ProtInc
Cellular macromolecule biosynthetic process Source: ParkinsonsUK-UCL
Heart development Source: Ensembl
Negative regulation of autophagy Source: ParkinsonsUK-UCL
Positive regulation of axon extension Source: Ensembl
Positive regulation of cell growth Source: ParkinsonsUK-UCL
Positive regulation of dendritic spine development Source: Ensembl
Positive regulation of translation Source: Ensembl
Regulation of cell cycle Source: ProtInc
Regulation of translational initiation Source: UniProtKB
Cellular Location
Cytosol; Adherens junction; Axon; Eukaryotic translation initiation factor 4F complex; Membrane
PTM
Phosphorylation; hyperphosphorylated during mitosis.

Smirnova, V. V., Shestakova, E. D., Nogina, D. S., Mishchenko, P. A., Prikazchikova, T. A., Zatsepin, T. S., ... & Terenin, I. M. (2022). Ribosomal leaky scanning through a translated uORF requires eIF4G2. Nucleic acids research, 50(2), 1111-1127.

Chen, K., Xi, M., Huang, Q., Wu, H., Lu, G., Song, S., & Shi, W. (2022). Long non-coding RNA MCM3AP antisense RNA 1 silencing upregulates microRNA-24-3p to accelerate proliferation and migration of vascular endothelial cells in myocardial infarction rats by reducing EIF4G2. Cell Cycle, 21(7), 674-684.

Li, S., Shao, J., Lou, G., Wu, C., Liu, Y., & Zheng, M. (2021). MiR-144-3p-mediated dysregulation of EIF4G2 contributes to the development of hepatocellular carcinoma through the ERK pathway. Journal of Experimental & Clinical Cancer Research, 40(1), 1-14.

Zhang, J., Zhang, R., & Ye, Y. (2021). Long non-coding RNA (LncRNA) SNHG7/Eukaryotic translation initiation factor 4 gamma 2 (EIF4G2) involves in the malignant events of ovarian cancer cells with paclitaxel resistant. Bioengineered, 12(2), 10541-10552.

Zhao, H., Wang, A., & Zhang, Z. (2020). LncRNA SDHAP1 confers paclitaxel resistance of ovarian cancer by regulating EIF4G2 expression via miR-4465. The Journal of Biochemistry, 168(2), 171-181.

Gao, S., Liu, L., Zhu, S., Wang, D., Wu, Q., Ning, G., & Feng, S. (2020). MicroRNA-197 regulates chondrocyte proliferation, migration, and inflammation in pathogenesis of osteoarthritis by targeting EIF4G2. Bioscience Reports, 40(9).

Wang, J., Lin, Z., Yang, Z., & Liu, X. (2020). lncRNA Eif4g2 improves palmitate-induced dysfunction of mouse β-cells via modulation of Nrf2 activation. Experimental cell research, 396(2), 112291.

Zhang, X., Sun, Z., & Zou, Y. (2020). LncRNA NEAT1 exacerbates non-small cell lung cancer by upregulating EIF4G2 via miR-582-5p sponging. Archives of Biological Sciences, 72(2), 243-252.

Chai, Y., & Xie, M. (2019). LINC01579 promotes cell proliferation by acting as a ceRNA of miR‐139‐5p to upregulate EIF4G2 expression in glioblastoma. Journal of cellular physiology, 234(12), 23658-23666.

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For research use only. Not intended for any clinical use.

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