Rabbit Anti-GPR75 Recombinant Antibody (EG1407) (CBMAB-EN1686-LY)

The product is antibody recognizes GPR75. The antibody EG1407 immunoassay techniques such as: WB: 1:500~1:1000 IF: 1:100~1:500 ELISA: 1:5000.
See all GPR75 antibodies

Datasheet

Summary

Host Animal

Rabbit

Specificity

Human, Mouse

Clone

EG1407

Antibody Isotype

IgG

Application

WB: 1:500~1:1000 IF: 1:100~1:500 ELISA: 1:5000

Basic Information

Immunogen

The antibody was produced against synthesized peptide derived from internal of human GPR75.

Specificity

Human, Mouse

Antibody Isotype

IgG

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name

G protein-coupled receptor 75

Introduction

GPR75 is a member of the G protein-coupled receptor family. GPRs are cell surface receptors that activate guanine-nucleotide binding proteins upon the binding of a ligand.

Entrez Gene ID

Human	81491
Mouse	81006

UniProt ID

Human	O95800
Mouse	Q6X632

Alternative Names

GPRchr2; WI31133

Function

G protein-coupled receptor that is activated by the chemokine CCL5/RANTES. Probably coupled to heterotrimeric Gq proteins, it stimulates inositol trisphosphate production and calcium mobilization upon activation. Together with CCL5/RANTES, may play a role in neuron survival through activation of a downstream signaling pathway involving the PI3, Akt and MAP kinases. CCL5/RANTES may also regulate insulin secretion by pancreatic islet cells through activation of this receptor.

Biological Process

Chemokine-mediated signaling pathway Source: UniProtKB
G protein-coupled receptor signaling pathway Source: UniProtKB
Regulation of neuron death Source: UniProtKB

Cellular Location

Cell membrane

Topology

Extracellular: 1-46
Helical: 47-67
Cytoplasmic: 68-86
Helical: 87-107
Extracellular: 108-120
Helical: 121-141
Cytoplasmic: 142-160
Helical: 161-181
Extracellular: 182-205
Helical: 206-226
Cytoplasmic: 227-318
Helical: 319-339
Extracellular: 340-350
Helical: 351-371
Cytoplasmic: 372-540

References

Speidell, A., Walton, S., Campbell, L. A., Tomassoni‐Ardori, F., Tessarollo, L., Corbo, C., ... & Mocchetti, I. (2023). Mice deficient for G‐protein coupled receptor 75 display altered presynaptic structural protein expression and disrupted fear conditioning recall. Journal of Neurochemistry.

D'Addario, C. (2022). The Role of G-Protein Coupled Receptor 75 in Pulmonary Hypertension.

Froogh, G., Garcia, V., & Schwartzman, M. L. (2022). The CYP/20-HETE/GPR75 axis in hypertension. In Advances in Pharmacology (Vol. 94, pp. 1-25). Academic Press.

D'Addario, C., Kitagawa, A., Matsumura, S., Zhang, F., Zeldin, D., Gruzdev, A., ... & Gupte, S. A. (2022). Deletion Of G‐Protein‐Coupled Receptor 75 Prevents Hypoxia‐Induced Pulmonary Vasoconstriction and Hypertension. The FASEB Journal, 36.

D’Addario, C., Kitagawa, A., Zhang, F., Zeldin, D., Gruzdev, A., Garcia, V., ... & Gupte, S. (2020). G Protein Coupled Receptor 75 and Its Role in Pulmonary Hypertension. The FASEB Journal, 34(S1), 1-1.

Dedoni, S., Campbell, L. A., Harvey, B. K., Avdoshina, V., & Mocchetti, I. (2018). The orphan G‐protein‐coupled receptor 75 signaling is activated by the chemokine CCL 5. Journal of neurochemistry, 146(5), 526-539.

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Protocols

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Associated Products

Related Products

Mouse Anti-GPR75 Recombinant Antibody (689705)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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