Mouse Anti-GPR75 Recombinant Antibody (689705) (CBMAB-G0721-LY)

This product is antibody recognizes GPR75. The antibody 689705 immunoassay techniques such as: IHC.
See all GPR75 antibodies

Datasheet

Summary

Host Animal

Mouse

Specificity

Human

Clone

689705

Antibody Isotype

IgG1

Application

IHC

Basic Information

Immunogen

NS0 mouse myeloma cell line transfected with human GPR75, Accession # O95800

Specificity

Human

Antibody Isotype

IgG1

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

lyophilized

Buffer

Trehalose

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name

G protein-coupled receptor 75

Introduction

GPR75 is a member of the G protein-coupled receptor family. GPRs are cell surface receptors that activate guanine-nucleotide binding proteins upon the binding of a ligand.[supplied by OMIM, Jul 2002]

Entrez Gene ID

10936

UniProt ID

O95800

Alternative Names

G Protein-Coupled Receptor 75; Probable G-Protein Coupled Receptor 75; GPRchr2; WI31133;

Function

G protein-coupled receptor that is activated by the chemokine CCL5/RANTES. Probably coupled to heterotrimeric Gq proteins, it stimulates inositol trisphosphate production and calcium mobilization upon activation. Together with CCL5/RANTES, may play a role in neuron survival through activation of a downstream signaling pathway involving the PI3, Akt and MAP kinases. CCL5/RANTES may also regulate insulin secretion by pancreatic islet cells through activation of this receptor.

Biological Process

Chemokine-mediated signaling pathway Source: UniProtKB
G protein-coupled receptor signaling pathway Source: UniProtKB
Regulation of neuron death Source: UniProtKB

Cellular Location

Cell membrane

Topology

Extracellular: 1-46
Helical: 47-67
Cytoplasmic: 68-86
Helical: 87-107
Extracellular: 108-120
Helical: 121-141
Cytoplasmic: 142-160
Helical: 161-181
Extracellular: 182-205
Helical: 206-226
Cytoplasmic: 227-318
Helical: 319-339
Extracellular: 340-350
Helical: 351-371
Cytoplasmic: 372-540

References

Speidell, A., Walton, S., Campbell, L. A., Tomassoni‐Ardori, F., Tessarollo, L., Corbo, C., ... & Mocchetti, I. (2023). Mice deficient for G‐protein coupled receptor 75 display altered presynaptic structural protein expression and disrupted fear conditioning recall. Journal of Neurochemistry.

D'Addario, C. (2022). The Role of G-Protein Coupled Receptor 75 in Pulmonary Hypertension.

Froogh, G., Garcia, V., & Schwartzman, M. L. (2022). The CYP/20-HETE/GPR75 axis in hypertension. In Advances in Pharmacology (Vol. 94, pp. 1-25). Academic Press.

D'Addario, C., Kitagawa, A., Matsumura, S., Zhang, F., Zeldin, D., Gruzdev, A., ... & Gupte, S. A. (2022). Deletion Of G‐Protein‐Coupled Receptor 75 Prevents Hypoxia‐Induced Pulmonary Vasoconstriction and Hypertension. The FASEB Journal, 36.

D’Addario, C., Kitagawa, A., Zhang, F., Zeldin, D., Gruzdev, A., Garcia, V., ... & Gupte, S. (2020). G Protein Coupled Receptor 75 and Its Role in Pulmonary Hypertension. The FASEB Journal, 34(S1), 1-1.

Dedoni, S., Campbell, L. A., Harvey, B. K., Avdoshina, V., & Mocchetti, I. (2018). The orphan G‐protein‐coupled receptor 75 signaling is activated by the chemokine CCL 5. Journal of neurochemistry, 146(5), 526-539.

Q & As

Ask a question We look forward to hearing from you.

0 reviews or Q&As

Purchasing FAQs
Technical FAQs

Review & reward

Have you used Mouse Anti-GPR75 Recombinant Antibody (689705)?
Submit a review and get a Coupon or an Amazon gift card. 20% off Coupon

$30 eGift Card
Submit a review

Protocols

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Associated Products

Related Products

Rabbit Anti-GPR75 Recombinant Antibody (EG1407)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

Online Inquiry

Documents

Datasheet
SDS
Request for COA

Request bulk or custom quote

Second antibody and isotype control

Contact us

Tel: (USA)
(UK)
Fax:

Global Locations

Email: