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Mouse Anti-IMPDH2 Recombinant Antibody (1E12-B6) (CBMAB-A4455-LY)

The product is antibody recognizes IMPDH2. The antibody 1E12-B6 immunoassay techniques such as: WB, ELISA.
See all IMPDH2 antibodies
Published Data
Fig.1 The relative band quantification of Western blot for IMPDH2 protein in PCa and non-cancerous prostate tissues.
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Summary

Host Animal
Mouse
Specificity
Human
Clone
1E12-B6
Antibody Isotype
IgG1, κ
Application
WB, ELISA

Basic Information

Immunogen
IMPDH2 (AAH12840, 1 a.a. ~ 514 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Specificity
Human
Antibody Isotype
IgG1, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Purity
> 95% Purity determined by SDS-PAGE.
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
IMP (inosine monophosphate) dehydrogenase 2
Introduction
This gene encodes the rate-limiting enzyme in the de novo guanine nucleotide biosynthesis. It is thus involved in maintaining cellular guanine deoxy- and ribonucleotide pools needed for DNA and RNA synthesis. The encoded protein catalyzes the NAD-dependent oxidation of inosine-5'-monophosphate into xanthine-5'-monophosphate, which is then converted into guanosine-5'-monophosphate. This gene is up-regulated in some neoplasms, suggesting it may play a role in malignant transformation. [provided by RefSeq]
Entrez Gene ID
3615
UniProt ID
P12268
Alternative Names
IMPD2; IMPDH-II
Function
Catalyzes the conversion of inosine 5'-phosphate (IMP) to xanthosine 5'-phosphate (XMP), the first committed and rate-limiting step in the de novo synthesis of guanine nucleotides, and therefore plays an important role in the regulation of cell growth (PubMed:7903306, PubMed:7763314).
Could also have a single-stranded nucleic acid-binding activity and could play a role in RNA and/or DNA metabolism (PubMed:14766016).
It may also have a role in the development of malignancy and the growth progression of some tumors.
Biological Process
de novo' XMP biosynthetic processIEA:Ensembl
Cellular response to interleukin-4IEA:Ensembl
Circadian rhythmManual Assertion Based On ExperimentIDA:UniProtKB
GMP biosynthetic processIEA:UniProtKB-UniRule
GTP biosynthetic processManual Assertion Based On ExperimentIDA:UniProtKB
Lymphocyte proliferationIEA:Ensembl
Retina development in camera-type eyeIEA:Ensembl
Cellular Location
Cytoplasm; Nucleus; Cytoplasm, cytosol. Can form fiber-like subcellular structures termed 'cytoophidia' in response to intracellular guanine-nucleotide depletion.
PTM
Ubiquitinated leading to its degradation by the proteasome.
The N-terminus is blocked.
Acetylated by CLOCK in a circadian manner (PubMed:28985504).

Lin, J., Li, C., Cui, Y., Hou, Y., Zhang, L., Ou, R., ... & Shang, H. (2023). Rare variants in IMPDH2 cause autosomal dominant dystonia in Chinese population. Journal of Neurology, 270(4), 2197-2203.

Wang, S., Chao, F., Zhang, C., Han, D., Xu, G., & Chen, G. (2022). Circular RNA circPFKP promotes cell proliferation by activating IMPDH2 in prostate cancer. Cancer letters, 524, 109-120.

Xu, H., Ma, H., Zha, L., Li, Q., Pan, H., & Zhang, L. (2022). Genistein promotes apoptosis of lung cancer cells through the IMPDH2/AKT1 pathway. American journal of translational research, 14(10), 7040.

Wang, W., Wu, Y., Chen, S., Liu, X., He, J., Wang, S., ... & Huang, J. (2021). Shikonin is a novel and selective IMPDH2 inhibitor that target triple‐negative breast cancer. Phytotherapy Research, 35(1), 463-476.

Kuukasjärvi, A., Landoni, J. C., Kaukonen, J., Juhakoski, M., Auranen, M., Torkkeli, T., ... & Suomalainen, A. (2021). IMPDH2: a new gene associated with dominant juvenile-onset dystonia-tremor disorder. European Journal of Human Genetics, 29(12), 1833-1837.

Kofuji, S., & Sasaki, A. T. (2020). GTP metabolic reprogramming by IMPDH2: unlocking cancer cells’ fuelling mechanism. The journal of biochemistry, 168(4), 319-328.

Johnson, M. C., & Kollman, J. M. (2020). Cryo-EM structures demonstrate human IMPDH2 filament assembly tunes allosteric regulation. Elife, 9, e53243.

Zheng, P., & Li, L. (2020). FANCI cooperates with IMPDH2 to promote lung adenocarcinoma tumor growth via a MEK/ERK/MMPs pathway. OncoTargets and therapy, 451-463.

Hayward, D., Kouznetsova, V. L., Pierson, H. E., Hasan, N. M., Guzman, E. R., Tsigelny, I. F., & Lutsenko, S. (2019). ANKRD9 is a metabolically-controlled regulator of IMPDH2 abundance and macro-assembly. Journal of Biological Chemistry, 294(39), 14454-14466.

Duan, S., Huang, W., Liu, X., Liu, X., Chen, N., Xu, Q., ... & Zhou, J. (2018). IMPDH2 promotes colorectal cancer progression through activation of the PI3K/AKT/mTOR and PI3K/AKT/FOXO1 signaling pathways. Journal of Experimental & Clinical Cancer Research, 37(1), 1-16.

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For research use only. Not intended for any clinical use.

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