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Mouse Anti-NFIX Recombinant Antibody (3D2) (CBMAB-N2233-WJ)

This product is a Mouse antibody that recognizes NFIX. The antibody 3D2 can be used for immunoassay techniques such as: ELISA, WB.
See all NFIX antibodies
Published Data
Fig.1 GFAP fluorescence intensity was reduced in the spinal cords of Nfix-/- mice at E18.5.
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Fig.2 A transverse section of an embryonic spinal cord from an E14.5 wild-type mouse at high magnification, labelled with the neural progenitor cell marker NFIX (red).
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Fig.3 NFIX immunohistochemostry performed in 1-week-old mice.
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Summary

Host Animal
Mouse
Specificity
Human
Clone
3D2
Antibody Isotype
IgG2a, κ
Application
ELISA, WB

Basic Information

Specificity
Human
Antibody Isotype
IgG2a, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, pH 7.2
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
NFIX
Introduction
The protein encoded by this gene is a transcription factor that binds the palindromic sequence 5'-TTGGCNNNNNGCCAA-3 in viral and cellular promoters. The encoded protein can also stimulate adenovirus replication in vitro. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2012]
Entrez Gene ID
4784
UniProt ID
Q14938
Alternative Names
Nuclear Factor I X; CCAAT-Box-Binding Transcription Factor; CCAAT-Binding Transcription Factor; TGGCA-Binding Protein; Nuclear Factor 1/X; NF-I/X; NF1-X; CTF;
Function
Recognizes and binds the palindromic sequence 5'-TTGGCNNNNNGCCAA-3' present in viral and cellular promoters and in the origin of replication of adenovirus type 2. These proteins are individually capable of activating transcription and replication.
Biological Process
DNA replicationIEA:UniProtKB-KW
Negative regulation of transcription by RNA polymerase IIManual Assertion Based On ExperimentIDA:UniProtKB
Positive regulation of transcription by RNA polymerase IIManual Assertion Based On ExperimentIDA:UniProtKB
Regulation of transcription by RNA polymerase IIManual Assertion Based On ExperimentIBA:GO_Central
Transcription by RNA polymerase IIManual Assertion Based On ExperimentTAS:ProtInc
Cellular Location
Nucleus
Involvement in disease
Sotos syndrome 2 (SOTOS2):
A form of Sotos syndrome, a childhood overgrowth syndrome characterized by prenatal and postnatal overgrowth, developmental delay, mental retardation, advanced bone age, and abnormal craniofacial morphology. SOTOS2 patients have macrocephaly, long narrow face, high forehead, slender habitus, scoliosis, and unusual behavior characterized especially by anxiety.
Marshall-Smith syndrome (MRSHSS):
A distinct malformation syndrome characterized by accelerated skeletal maturation, relative failure to thrive, respiratory difficulties, mental retardation, and unusual facies, including prominent forehead, shallow orbits, blue sclerae, depressed nasal bridge, and micrognathia. Additional skeletal findings include long and thin tubular bones, broad middle phalanges with relatively narrow distal phalanges, and scoliosis.

Ge, R., Wang, C., Liu, J., Jiang, H., Jiang, X., & Liu, Z. (2023). A novel tumor-promoting role for nuclear factor IX in glioblastoma is mediated through transcriptional activation of GINS1. Molecular Cancer Research, 21(3), 189-198.

Pan, D., Zhong, J., Zhang, J., Dong, H., Zhao, D., Zhang, H., & Yao, B. (2023). Function and regulation of nuclear factor 1 X-type on chondrocyte proliferation and differentiation. Gene, 881, 147620.

Kooblall, K. G., Stevenson, M., Stewart, M., Harris, L., Zalucki, O., Dewhurst, H., ... & Thakker, R. V. (2023). A Mouse Model with a Frameshift Mutation in the Nuclear Factor I/X (NFIX) Gene Has Phenotypic Features of Marshall‐Smith Syndrome. JBMR plus, 7(6), e10739.

Kawamoto, S., Hani, T., Fujita, K., Taya, Y., Sasaki, Y., Kudo, T., ... & Soeno, Y. (2023). Nuclear factor 1 X-type-associated regulation of myogenesis in developing mouse tongue. Journal of Oral Biosciences, 65(1), 88-96.

Walker, M. J. (2020). Characterization of Genome-Wide Binding of NUCLEAR-FACTOR IX in Hematopoietic Progenitor Cells.

Liu, Z., Ge, R., Zhou, J., Yang, X., Cheng, K. K. Y., Tao, J., ... & Mao, J. (2020). Nuclear factor IX promotes glioblastoma development through transcriptional activation of Ezrin. Oncogenesis, 9(4), 39.

Chaand, M., Fiore, C., Johnston, B. T., Moon, D. H., Carulli, J. P., & Shearstone, J. R. (2019). Chromatin accessibility mapping of primary erythroid cell populations leads to identification and validation of nuclear factor IX (NFIX) as a novel fetal hemoglobin (HbF) repressor. Blood, 134, 812.

Piper, M., Gronostajski, R., & Messina, G. (2019). Nuclear factor one X in development and disease. Trends in cell biology, 29(1), 20-30.

Priolo, M. (2019). Nuclear Factor One X Mice model for Malan syndrome: the less the better. EBioMedicine, 39, 15-16.

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For research use only. Not intended for any clinical use.

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