Mouse Anti-NFIX Antibody (2D3) (CBMAB-0725-YC)

Name: Mouse Anti-NFIX Antibody (2D3)
SKU: CBMAB-0725-YC
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

2D3

Application

IP, MA

Immunogen

Recombinant protein

Specificity

Human

Antibody Isotype

IgG2b

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Supernatant

Storage

Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

NFIX

Introduction

NFIX (nuclear factor 1 X-type) is a transcription factor that binds the palindromic sequence 5'-TTGGCNNNNNGCCAA-3 in viral and cellular promoters. NFIX can also stimulate adenovirus replication in vitro.

Entrez Gene ID

4784

UniProt ID

Q14938

Alternative Names

CTF; NF1A; NF1-X; MRSHSS; NF-I/X; SOTOS2

Function

Recognizes and binds the palindromic sequence 5'-TTGGCNNNNNGCCAA-3' present in viral and cellular promoters and in the origin of replication of adenovirus type 2. These proteins are individually capable of activating transcription and replication.

Biological Process

DNA replicationIEA:UniProtKB-KW
Negative regulation of transcription by RNA polymerase IIManual Assertion Based On ExperimentIDA:UniProtKB
Positive regulation of transcription by RNA polymerase IIManual Assertion Based On ExperimentIDA:UniProtKB
Regulation of transcription by RNA polymerase IIManual Assertion Based On ExperimentIBA:GO_Central
Transcription by RNA polymerase IIManual Assertion Based On ExperimentTAS:ProtInc

Cellular Location

Nucleus

Involvement in disease

Sotos syndrome 2 (SOTOS2):
A form of Sotos syndrome, a childhood overgrowth syndrome characterized by prenatal and postnatal overgrowth, developmental delay, mental retardation, advanced bone age, and abnormal craniofacial morphology. SOTOS2 patients have macrocephaly, long narrow face, high forehead, slender habitus, scoliosis, and unusual behavior characterized especially by anxiety.
Marshall-Smith syndrome (MRSHSS):
A distinct malformation syndrome characterized by accelerated skeletal maturation, relative failure to thrive, respiratory difficulties, mental retardation, and unusual facies, including prominent forehead, shallow orbits, blue sclerae, depressed nasal bridge, and micrognathia. Additional skeletal findings include long and thin tubular bones, broad middle phalanges with relatively narrow distal phalanges, and scoliosis.

Ge, R., Wang, C., Liu, J., Jiang, H., Jiang, X., & Liu, Z. (2023). A novel tumor-promoting role for nuclear factor IX in glioblastoma is mediated through transcriptional activation of GINS1. Molecular Cancer Research, 21(3), 189-198.

Pan, D., Zhong, J., Zhang, J., Dong, H., Zhao, D., Zhang, H., & Yao, B. (2023). Function and regulation of nuclear factor 1 X-type on chondrocyte proliferation and differentiation. Gene, 881, 147620.

Kooblall, K. G., Stevenson, M., Stewart, M., Harris, L., Zalucki, O., Dewhurst, H., ... & Thakker, R. V. (2023). A Mouse Model with a Frameshift Mutation in the Nuclear Factor I/X (NFIX) Gene Has Phenotypic Features of Marshall‐Smith Syndrome. JBMR plus, 7(6), e10739.

Kawamoto, S., Hani, T., Fujita, K., Taya, Y., Sasaki, Y., Kudo, T., ... & Soeno, Y. (2023). Nuclear factor 1 X-type-associated regulation of myogenesis in developing mouse tongue. Journal of Oral Biosciences, 65(1), 88-96.

Walker, M. J. (2020). Characterization of Genome-Wide Binding of NUCLEAR-FACTOR IX in Hematopoietic Progenitor Cells.

Liu, Z., Ge, R., Zhou, J., Yang, X., Cheng, K. K. Y., Tao, J., ... & Mao, J. (2020). Nuclear factor IX promotes glioblastoma development through transcriptional activation of Ezrin. Oncogenesis, 9(4), 39.

Chaand, M., Fiore, C., Johnston, B. T., Moon, D. H., Carulli, J. P., & Shearstone, J. R. (2019). Chromatin accessibility mapping of primary erythroid cell populations leads to identification and validation of nuclear factor IX (NFIX) as a novel fetal hemoglobin (HbF) repressor. Blood, 134, 812.

Piper, M., Gronostajski, R., & Messina, G. (2019). Nuclear factor one X in development and disease. Trends in cell biology, 29(1), 20-30.

Priolo, M. (2019). Nuclear Factor One X Mice model for Malan syndrome: the less the better. EBioMedicine, 39, 15-16.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Related Products

Mouse Anti-NFIX Recombinant Antibody (3D2) (CAT#: CBMAB-N2233-WJ)

Isotype control

For research use only. Not intended for any clinical use.

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