Ly6G Antibodies

Structure of Ly6G

Ly6G is a glycosylated protein with a molecular weight of approximately 20-25 kDa. Its specific molecular weight varies slightly among different species due to differences in glycosylation modifications and amino acid sequences.

This protein belongs to the LY6/uPAR superfamily. Its structure is mainly composed of a highly conserved LU domain, forming a typical three-finged-like fold and stabilizing the spatial conformation through disulfide bonds. Its C-terminal is anchored to the cell membrane by glycosylphosphatidylinositol (GPI), responsible for binding to ligands and participating in membrane signal transduction. This structural feature contributes to its specific recognition and high-density expression on the surface of immune cells.

Fig. 1 Both Ly6G+ neutrophils and IL-17+ T cells are key cell subsets in resistance to Plasmodium infection.¹

Key structural properties of Ly6G:

• Conservative three-fingered LU domain
• GPI anchor is due to the cell membrane surface
• Highly glycosylated carboxyl terminus

Functions of Ly6G

The main function of the Ly6G protein is to serve as a specific surface marker for neutrophils and participate in immune regulation. Its specific functions include:

Ly6G has a high affinity for ligand binding and is specific. Its recognition process does not rely on the major histocompatibility complex (MHC), demonstrating its unique role in rapid innate immunity.

Applications of Ly6G and Ly6G Antibody in Literature

1. Ruscitti, Cecilia, et al. "Recruited atypical Ly6G+ macrophages license alveolar regeneration after lung injury." Science immunology 9.98 (2024): eado1227. https://doi.org/10.1126/sciimmunol.ado1227

The article indicates that after lung injury, mononuclear derived Ly6G+ macrophages aggregate around the lesion, clear immune cells, and mutually promote regeneration with alveolar type II epithelial cells. This cell population depends on the GM-CSF/IL-4 signaling and is crucial for epithelial repair, and can serve as a new target for the treatment of lung injury.

2. Deyhle, Michael R., et al. "Depleting Ly6G positive myeloid cells reduces pancreatic cancer-induced skeletal muscle atrophy." Cells 11.12 (2022): 1893. https://doi.org/10.3390/cells11121893

The article indicates that in a mouse model of pancreatic cancer, treatment with anti-LY6G antibodies effectively eliminated Ly6G+ cells (including granulocytoid MDSCS and neutrophils) in tumors and skeletal muscles, significantly delaying weight loss, reducing muscle atrophy and the expression of atrophie-related genes, suggesting that Ly6G+ cells may promote skeletal muscle consumption in cancerous cachexia.

3. Wang, Heng, et al. "Highly sensitive magnetic particle imaging of abdominal aortic aneurysm NETosis with anti-Ly6G iron oxide nanoparticles." Cell death discovery 10.1 (2024): 395. https://doi.org/10.1038/s41420-024-02156-3

The article indicates that for neutrophil infiltration in abdominal aortic aneurysms (AAA), multimodal imaging nanoparticles targeting Ly6G (Ly6G NPs) have been studied and developed. Precise localization and severity monitoring of AAA lesions are achieved through techniques such as magnetic particle imaging (MPI). It was confirmed that inhibiting NETosis could reduce the aggregation of Ly6G+ cells and aortic injury.

4. Kleinholz, Corinna L., et al. "Ly6G deficiency alters the dynamics of neutrophil recruitment and pathogen capture during Leishmania major skin infection." Scientific reports 11.1 (2021): 15071. https://doi.org/10.1038/s41598-021-94425-9

The article indicates that the absence of Ly6G can delay the early recruitment of neutrophils to the infection site, reduce the initial infection rate of Leishmania, but does not affect the uptake of neutrophil substances by monocytes. Studies have shown that Ly6G enhances the early control of intracellular pathogens by the immune system by promoting the timely entry of neutrophils into the infection site.

5. Su, Ziwei, et al. "Ly6G+ Neutrophils and Interleukin-17 Are Essential in Protection against Rodent Malaria Caused by Plasmodium berghei ANKA." Research 7 (2024): 0559. https://doi.org/10.34133/research.0559

The article indicates that in the face of Plasmodium bergii infection, Ly6G+ neutrophils significantly increase, and their absence enhances the susceptibility to infection. IL-17 exerts a protective effect by amplifying the Ly6G+ population, while dihydroartemisinin (DHA) can enhance the antimalarial function of this group of cells through the CD18/CXCR4 pathway.

Creative Biolabs: Ly6G Antibodies for Research

Creative Biolabs specializes in the production of high-quality Ly6G antibodies for research and industrial applications. Our portfolio includes monoclonal antibodies tailored for ELISA, Flow Cytometry, Western blot, immunohistochemistry, and other diagnostic methodologies.

• Custom Ly6G Antibody Development: Tailor-made solutions to meet specific research requirements.
• Bulk Production: Large-scale antibody manufacturing for industry partners.
• Technical Support: Expert consultation for protocol optimization and troubleshooting.
• Aliquoting Services: Conveniently sized aliquots for long-term storage and consistent experimental outcomes.

For more details on our Ly6G antibodies, custom preparations, or technical support, contact us at email.