Mouse Anti-ADGRL2 Recombinant Antibody (V2-58519) (CBMAB-L0166-YJ)

Name: Mouse Anti-ADGRL2 Recombinant Antibody (V2-58519)
SKU: CBMAB-L0166-YJ
Rating: 5 (1 reviews)

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Published Data

Mouse Anti-ADGRL2 Recombinant Antibody (CBYJL-109) (CBMAB-L0166-YJ) in IP

Immunoprecipitation (IP) of LPHN2 in whole HUVEC lysates treated with or without LRG1 (1 μg/ml) for 10 min followed by immunoblot analysis to detect LRG1 and LPHN2. Data are the means ± SEM (n = 3). ...View More

Yin, G. N., Kim, D. K., Kang, J. I., Im, Y., Lee, D. S., Han, A. R., ... & Kim, H. M. (2022). Latrophilin-2 is a novel receptor of LRG1 that rescues vascular and neurological abnormalities and restores diabetic erectile function. Experimental & Molecular Medicine, 54(5), 626-638.

Datasheet

Summary

Host Animal

Mouse

Specificity

Human

Clone

V2-58519

Antibody Isotype

IgG1, κ

Application

WB, IP, IF, ELISA

Basic Information

Immunogen

Raised against amino acids 326-370 mapping within an internal region of Latrophilin-2 of human origin.

Host Species

Mouse

Specificity

Human

Antibody Isotype

IgG1, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate).
IF(ICC)	1:50-1:500
ELISA	1:30-1:3,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Buffer

PBS, 0.1% gelatin.

Preservative

< 0.1% sodium azide

Concentration

0.2 mg/ml

Storage

Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name

Adhesion G Protein-Coupled Receptor L2

Introduction

ADGRL2 is a member of the latrophilin subfamily of G-protein coupled receptors. ADGRL2 participates in the regulation of exocytosis. The proprotein is thought to be further cleaved within a cysteine-rich G-protein-coupled receptor proteolysis site into two chains that are non-covalently bound at the cell membrane. Alternative splicing results in multiple transcript variants.

Entrez Gene ID

23266

UniProt ID

O95490

Alternative Names

Adhesion G Protein-Coupled Receptor L2; Calcium-Independent Alpha-Latrotoxin Receptor 2; Latrophilin Homolog 1; Latrophilin-2; Lectomedin-1; LPHH1; LPHN2; LEC1

Function

Calcium-independent receptor of low affinity for alpha-latrotoxin, an excitatory neurotoxin present in black widow spider venom which triggers massive exocytosis from neurons and neuroendocrine cells. Receptor probably implicated in the regulation of exocytosis.

Biological Process

Adenylate cyclase-activating G protein-coupled receptor signaling pathway
Brain development
Cell surface receptor signaling pathway
G protein-coupled receptor signaling pathway
Positive regulation of synapse assembly
Response to bacterium
Synapse organization

Cellular Location

Membrane

Topology

Extracellular: 26-851 aa
Helical: 852-872 aa
Cytoplasmic: 873-880 aa
Helical: 881-901 aa
Extracellular: 902-907 aa
Helical: 908-928 aa
Cytoplasmic: 929-952 aa
Helical: 953-973 aa
Extracellular: 974-990 aa
Helical: 991-1011 aa
Cytoplasmic: 1012-1037 aa
Helical: 1038-1058 aa
Extracellular: 1059-1062 aa
Helical: 1063-1083 aa
Cytoplasmic: 1084-1459 aa

PTM

Proteolytically cleaved into 2 subunits, an extracellular subunit and a seven-transmembrane subunit.

More Infomation

References

Bucar, E., McWilliams, C., & Frey, M. (2020). Adhesion G‐Protein‐Coupled Receptor L2 Expression is Lost in Colorectal Cancer but is Also Associated with Colonic Stem Cells. The FASEB Journal, 34(S1), 1-1.

Kang, H. C., Kim, H. S., Lee, J. B., Yoo, C. K., Choi, T. J., & Lim, H. T. (2018). Analysis of the relationship between the end weight trait and the gene ADGRL2 in purebred landrace pigs using a Genome-wide association study. Korean Journal of Agricultural Science, 45(2), 238-247.

Vezain, M., Lecuyer, M., Rubio, M., Dupé, V., Ratié, L., David, V., ... & Saugier-Veber, P. (2018). A de novo variant in ADGRL2 suggests a novel mechanism underlying the previously undescribed association of extreme microcephaly with severely reduced sulcation and rhombencephalosynapsis. Acta neuropathologica communications, 6(1), 1-23.

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Protocols

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

Associated Products

Related Products

Mouse Anti-ADGRL2 Recombinant Antibody (V2-58650)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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Documents

Datasheet
SDS
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Second antibody and isotype control

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