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Mouse Anti-BMI1 Recombinant Antibody (CBYC-P026) (CBMAB-P0108-YC)

Provided herein is a Mouse monoclonal antibody against Human BMI1 Proto-Oncogene, Polycomb Ring Finger. The antibody can be used for immunoassay techniques, such as WB, IHC, IF, ICC.
See all BMI1 antibodies

Summary

Host Animal
Mouse
Specificity
Human, Mouse
Clone
CBYC-P026
Antibody Isotype
IgG2a
Application
WB, IHC, IF, ICC

Basic Information

Immunogen
Recombinant Polycomb complex protein BMI-1 protein
Specificity
Human, Mouse
Antibody Isotype
IgG2a
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
TBS, pH 7.4, 0.5% BSA, 40% glycerol, 0.05% sodium azide
Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at-20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
BMI1 Proto-Oncogene, Polycomb Ring Finger
Introduction
BMI1 is a ring finger protein that is major component of the polycomb group complex 1 (PRC1). This complex functions through chromatin remodeling as an essential epigenetic repressor of multiple regulatory genes involved in embryonic development and self-renewal in somatic stem cells. This protein also plays a central role in DNA damage repair. This gene is an oncogene and aberrant expression is associated with numerous cancers and is associated with resistance to certain chemotherapies. A pseudogene of this gene is found on chromosome X. Read-through transcription also exists between this gene and the upstream COMM domain containing 3 (COMMD3) gene.
Entrez Gene ID
Human648
Mouse12151
UniProt ID
HumanP35226
MouseP25916
Alternative Names
BMI1 Proto-Oncogene, Polycomb Ring Finger; Polycomb Group RING Finger Protein 4; PCGF4; RNF51; B Lymphoma Mo-MLV Insertion Region 1 Homolog (Mouse); Murine Leukemia Viral (Bmi-1) Oncogene Homolog; B Lymphoma Mo-MLV Insertion Region 1 Homolog; BMI1 Polycomb Ring Finger Proto-Oncogene; BMI1 Polycomb Ring Finger Oncogene;
Function
Component of a Polycomb group (PcG) multiprotein PRC1-like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility (PubMed:15386022, PubMed:16359901, PubMed:26151332, PubMed:16714294, PubMed:21772249, PubMed:25355358, PubMed:27827373).
The complex composed of RNF2, UB2D3 and BMI1 binds nucleosomes, and has activity only with nucleosomal histone H2A (PubMed:21772249, PubMed:25355358).
In the PRC1-like complex, regulates the E3 ubiquitin-protein ligase activity of RNF2/RING2 (PubMed:15386022, PubMed:26151332, PubMed:21772249).
Biological Process
Chromatin silencing Source: GO_Central
Hemopoiesis Source: UniProtKB
Histone H2A-K119 monoubiquitination Source: UniProtKB
Negative regulation of G0 to G1 transition Source: Reactome
Negative regulation of gene expression, epigenetic Source: UniProtKB
Negative regulation of transcription by RNA polymerase II Source: UniProtKB
Positive regulation of fibroblast proliferation Source: BHF-UCL
Positive regulation of ubiquitin-protein transferase activity Source: UniProtKB
Regulation of gene expression Source: BHF-UCL
Segment specification Source: ProtInc
Cellular Location
Nucleus; Cytoplasm
PTM
Monoubiquitinated (By similarity). May be polyubiquitinated; which does not lead to proteasomal degradation.

Fitieh, A., Locke, A. J., Motamedi, M., & Ismail, I. H. (2021). The Role of Polycomb Group Protein BMI1 in DNA Repair and Genomic Stability. International Journal of Molecular Sciences, 22(6), 2976.

Wu, D., He, X., Wang, W., Hu, X., Wang, K., & Wang, M. (2020). Long noncoding RNA SNHG12 induces proliferation, migration, epithelial–mesenchymal transition, and stemness of esophageal squamous cell carcinoma cells via post‐transcriptional regulation of BMI1 and CTNNB1. Molecular oncology, 14(9), 2332-2351.

Solorzano-Vargas, R. S., Bjerknes, M., Wu, S. V., Wang, J., Stelzner, M., Dunn, J. C., ... & Martín, M. G. (2019). The cellular regulators PTEN and BMI1 help mediate NEUROGENIN-3–induced cell cycle arrest. Journal of Biological Chemistry, 294(41), 15182-15192.

Wu, L., Zhang, D., Zhou, L., Pei, Y., Zhuang, Y., Cui, W., & Chen, J. (2019). FUN14 domain-containing 1 promotes breast cancer proliferation and migration by activating calcium-NFATC1-BMI1 axis. EBioMedicine, 41, 384-394.

Li, P., Liu, D., Mei, Q., Wang, X., Zheng, Y., Pan, H., & Zheng, L. (2018, June). Research on the Effect of BMI1 Gene Silencing on the Proliferation Activity of Human Breast Cancer Cells. In 2018 8th International Conference on Mechatronics, Computer and Education Informationization (MCEI 2018) (pp. 88-93). Atlantis Press.

Vaishnave, S. (2018). BMI1 and PTEN are key determinants of breast cancer therapy: A plausible therapeutic target in breast cancer. Gene, 678, 302-311.

Koh, H., Park, H., Chandimali, N., Huynh, D. L., Zhang, J. J., Ghosh, M., ... & Jeong, D. K. (2017). MicroRNA-128 suppresses paclitaxel-resistant lung cancer by inhibiting MUC1-C and BMI-1 in cancer stem cells. Oncotarget, 8(66), 110540.

Liu, X., Wei, W., Li, X., Shen, P., Ju, D., Wang, Z., ... & Xi, R. (2017). BMI1 and MEL18 promote colitis-associated cancer in mice via REG3B and STAT3. Gastroenterology, 153(6), 1607-1620.

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For research use only. Not intended for any clinical use.

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